Mouse Anti-Porcine CD8α-FITC (76-2-11)
Cat. No.:
4520-02
FITC Anti-Porcine CD8α for use in flow cytometry and immunohistochemistry assays.
$413.00
Clone | 76-2-11 |
---|---|
Isotype | Mouse (BALB/c) IgG2aκ |
Isotype Control | Mouse IgG2a-FITC (HOPC-1) |
Specificity | Porcine CD8α |
Description | Porcine CD8, a type I transmembrane glycoprotein, is expressed as either a CD8αα homodimer or a CD8αβ heterodimer. It is found on most thymocytes and on the suppressor/cytotoxic subpopulation of peripheral T cells. CD8 functions primarily as a co-receptor with MHC Class I-restricted T cell receptors in antigen recognition. The monoclonal antibody 76-2-11 reacts with the CD8 α-chain. |
Immunogen | Fresh dd miniature swine thymocytes |
Conjugate | FITC (Fluorescein) |
Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
Clonality | Monoclonal |
Concentration | 0.5 mg/mL |
Volume | 1.0 mL |
Recommended Storage | 2-8°C; Avoid exposure to light |
Applications |
Flow Cytometry – Quality tested 1,11-15,17-28 Immunohistochemistry-Frozen Sections – Reported in literature 2-6,16 Immunohistochemistry-Paraffin Sections – Reported in literature 7 Immunoprecipitation – Reported in literature 1 Blocking – Reported in literature 8,9 Depletion – Reported in literature 10 Complement Mediated Cell Depletion – Reported in literature 1 |
RRID Number | AB_2796031 |
Gene ID |
396627 (Porcine) |
Gene ID Symbol |
CD8A (Porcine) |
Documentation
Certificate of Analysis Lookup
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- 2. Minguez I, Rueda A, DomÃnguez J, Sánchez-VizcaÃno JM. Double labeling immunohistological study of African swine fever virus-infected spleen and lymph nodes. Vet Pathol. 1988;25:193-8. (IHC-FS)
- 3. Yamada K, Shimizu A, Ierino FL, Utsugi R, Barth RN, Esnaola N, et al. Thymic transplantation in miniature swine. I. Development and function of the "thymokidney". Transplantation. 1999;68:1684-92. (IHC-FS)
- 4. Shimizu A, Yamada K, Meehan SM, Sachs DH, Colvin RB. Acceptance reaction: intragraft events associated with tolerance to renal allografts in miniature swine. J Am Soc Nephrol. 2000;11:2371-80. (IHC-FS)
- 5. Shimizu A, Yamada K, Sachs DH, Colvin RB. Mechanisms of chronic renal allograft rejection. II. Progressive allograft glomerulopathy in miniature swine. Lab Invest. 2002;82:673-85. (IHC-FS)
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- 7. Jung K, Alekseev KP, Zhang X, Cheon D, Vlasova AN, Saif LJ. Altered pathogenesis of porcine respiratory coronavirus in pigs due to immunosuppressive effects of dexamethasone: implications for corticosteroid use in treatment of severe acute respiratory syndrome coronavirus. J Virol. 2007;81:13681-93. (IHC-PS)
- 8. Pescovitz MD, Lunney JK, Sachs DH. Murine anti-swine T4 and T8 monoclonal antibodies: distribution and effects on proliferative and cytotoxic T cells. J Immunol. 1985;134:37-44. (Block)
- 9. SaÃz JC, RodrÃguez A, González M, Alonso F, Sobrino F. Heterotypic lymphoproliferative response in pigs vaccinated with foot-and-mouth disease virus. Involvement of isolated capsid proteins. J Gen Virol. 1992;73:2601-7. (Block)
- 10. Suzuki T, Sundt TM 3rd, Mixon A, Sachs DH. In vivo treatment with antiporcine T cell antibodies. Transplantation. 1990;50:76-81. (Depletion)
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