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Mouse Anti-Porcine CD8α-AF488 (76-2-11)

Mouse Anti-Porcine CD8α-AF488 (76-2-11)

Name: Mouse Anti-Porcine CD8α-AF488 (76-2-11)
SKU: 4520-30
Price: 270 USD
Availability: InStock

Cat. No.:

4520-30

Alexa Fluor^® 488 Anti-Porcine CD8α for use in flow cytometry and immunohistochemistry assays.

$270.00

Size	Price (USD)	Quantity
0.1 mg	$270.00

Qty

Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone	76-2-11
Isotype	Mouse (BALB/c) IgG_2aκ
Isotype Control	Mouse IgG2a-AF488 (HOPC-1)
Specificity	Porcine CD8α
Description	Porcine CD8, a type I transmembrane glycoprotein, is expressed as either a CD8αα homodimer or a CD8αβ heterodimer. It is found on most thymocytes and on the suppressor/cytotoxic subpopulation of peripheral T cells. CD8 functions primarily as a co-receptor with MHC Class I-restricted T cell receptors in antigen recognition. The monoclonal antibody 76-2-11 reacts with the CD8 α-chain.
Immunogen	Fresh dd miniature swine thymocytes
Conjugate	AF488 (Alexa Fluor^® 488)
Buffer Formulation	Phosphate buffered saline containing < 0.1% sodium azide
Clonality	Monoclonal
Concentration	0.5 mg/mL
Volume	0.2 mL
Recommended Storage	2-8°C; Avoid exposure to light
Trademark Information	Alexa Fluor^{^®} is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications	Flow Cytometry – Quality tested ^{1,11-15,17-28} Immunohistochemistry-Frozen Sections – Reported in literature ^2-6,16 Immunohistochemistry-Paraffin Sections – Reported in literature ⁷ Immunoprecipitation – Reported in literature ¹ Blocking – Reported in literature ^8,9 Depletion – Reported in literature ¹⁰ Complement Mediated Cell Depletion – Reported in literature ¹
RRID Number	AB_2796036
Gene ID	396627 (Porcine)
Gene ID Symbol	CD8A (Porcine)

Documentation

Technical Bulletin Safety Datasheet

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Citations

1. Pescovitz MD, Lunney JK, Sachs DH. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J Immunol. 1984;133:368-375. (Immunogen, FC, IP, CMDC)
2. Minguez I, Rueda A, Domínguez J, Sánchez-Vizcaíno JM. Double labeling immunohistological study of African swine fever virus-infected spleen and lymph nodes. Vet Pathol. 1988;25:193-8. (IHC-FS)
3. Yamada K, Shimizu A, Ierino FL, Utsugi R, Barth RN, Esnaola N, et al. Thymic transplantation in miniature swine. I. Development and function of the "thymokidney". Transplantation. 1999;68:1684-92. (IHC-FS)
4. Shimizu A, Yamada K, Meehan SM, Sachs DH, Colvin RB. Acceptance reaction: intragraft events associated with tolerance to renal allografts in miniature swine. J Am Soc Nephrol. 2000;11:2371-80. (IHC-FS)
5. Shimizu A, Yamada K, Sachs DH, Colvin RB. Mechanisms of chronic renal allograft rejection. II. Progressive allograft glomerulopathy in miniature swine. Lab Invest. 2002;82:673-85. (IHC-FS)
6. Vega-López MA, Arenas-Contreras G, Bailey M, Gonzáles-Pozos S, Stokes CR, Ortega MG, et al. Development of intraepithelial cells in the porcine small intestine. Dev Immunol. 2001;8:147-58. (IHC-FS)
7. Jung K, Alekseev KP, Zhang X, Cheon D, Vlasova AN, Saif LJ. Altered pathogenesis of porcine respiratory coronavirus in pigs due to immunosuppressive effects of dexamethasone: implications for corticosteroid use in treatment of severe acute respiratory syndrome coronavirus. J Virol. 2007;81:13681-93. (IHC-PS)
8. Pescovitz MD, Lunney JK, Sachs DH. Murine anti-swine T4 and T8 monoclonal antibodies: distribution and effects on proliferative and cytotoxic T cells. J Immunol. 1985;134:37-44. (Block)
9. Saíz JC, Rodríguez A, González M, Alonso F, Sobrino F. Heterotypic lymphoproliferative response in pigs vaccinated with foot-and-mouth disease virus. Involvement of isolated capsid proteins. J Gen Virol. 1992;73:2601-7. (Block)
10. Suzuki T, Sundt TM 3^rd, Mixon A, Sachs DH. In vivo treatment with antiporcine T cell antibodies. Transplantation. 1990;50:76-81. (Depletion)
11. Davis ME, Maxwell CV, Erf GF, Brown DC, Wistuba TJ. Dietary supplementation with phosphorylated mannans improves growth response and modulates immune function of weanling pigs. J Anim Sci. 2004;82:1882-91. (FC)
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14. Toka FN, Nfon CK, Dawson H, Estes DM, Golde WT. Activation of porcine natural killer cells and lysis of foot-and-mouth disease virus infected cells. J Interferon Cytokine Res. 2009;29:179-92. (FC)
15. Martelli P, Gozio S, Ferrari L, Rosina S, De Angelis E, Quintavalla C, et al. Efficacy of a modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in pigs naturally exposed to a heterologous European (Italian cluster) field strain: Clinical protection and cell-mediated immunity. Vaccine. 2009;27:3788-99. (FC)
16. Diaz-San Segundo F, Moraes MP, de Los Santos T, Dias CC, Grubman MJ. Interferon-induced protection against foot-and-mouth disease virus infection correlates with enhanced tissue-specific innate immune cell infiltration and interferon-stimulated gene expression. J Virol. 2010;84:2063-77. (IHC-FS)
17. Ferrari L, De Angelis E, Martelli P, Borghetti P. Swine virus-specific cytotoxic cells producing IFNγ under different conditions of virus antigenic stimulation. Vet Res Commun. 2010;34:S63-7. (FC)
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19. Wen K, Li G, Zhang W, Azevedo MS, Saif LJ, Liu F, et al. Development of γδ T cell subset responses in gnotobiotic pigs infected with human rotaviruses and colonized with probiotic lactobacilli. Vet Immunol Immunopathol. 2011;141:267-75. (FC)
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22. Wen K, Bui T, Li G, Liu F, Li Y, Kocher J, et al. Characterization of immune modulating functions of γδ T cell subsets in a gnotobiotic pig model of human rotavirus infection. Comp Immunol Microbiol Infect Dis. 2012;35:289-301. (FC)
23. Liu KY, Comstock SS, Shunk JM, Monaco MH, Donovan SM. Natural killer cell populations and cytotoxic activity in pigs fed mother's milk, formula, or formula supplemented with bovine lactoferrin. Pediatr Res. 2013;74:402-7. (FC)
24. Thorum SC, Hester SN, Comstock SS, Monaco MH, Pence BD, Woods JA, et al. Dietary (1,3/1,6)-β-D-glucan decreases transforming growth factor β expression in the lung of the neonatal piglet. Nutr Res. 2013;33:322-31 (FC)
25. Liu Y, Che TM, Song M, Lee JJ, Almeida JA, Bravo D, et al. Dietary plant extracts improve immune responses and growth efficiency of pigs experimentally infected with porcine reproductive and respiratory syndrome virus. J Anim Sci. 2013;91:5668-79. (FC)
26. Ferrari L, Borghetti P, De Angelis E, Martelli P. Memory T cell proliferative responses and IFN-γ productivity sustain long-lasting efficacy of a Cap-based PCV2 vaccine upon PCV2 natural infection and associated disease. Vet Res. 2014;45:44. (FC)
27. Liu P, Pieper R, Tedin L, Martin L, Meyer W, Rieger J, et al. Effect of dietary zinc oxide on jejunal morphological and immunological characteristics in weaned piglets. J Anim S. 2014;92:5009-18. (FC)
28. Zhu Y, Li X, Zhang W, Zhou D, Liu H, Wang J. Dose-dependent effects of Lactobacillus rhamnosus on serum interleukin-17 production and intestinal T-cell responses in pigs challenged with Escherichia coli. Appl Environ Microbiol. 2014;80:1787-98. (FC)