My Cart
$0.00
0

Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

  • Home
  • Mouse Anti-Porcine CD4-UNLB (74-12-4)

Mouse Anti-Porcine CD4-UNLB (74-12-4)

Cat. No.:
4515-01
Purified Anti-Porcine CD4 for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, separation, blocking, and CMCD assays.
$303.00
Size Price (USD) Quantity
0.5 mg $303.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone 74-12-4
Isotype Mouse (BALB/c) IgG2bκ
Isotype Control Mouse IgG2b-UNLB (A-1)
Specificity Porcine CD4
Description Porcine CD4 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. CD4 is an accessory molecule in the recognition of foreign antigens in association with MHC Class II molecules by T lymphocytes.
Immunogen Fresh dd miniature swine thymocytes
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested 1,6,7,15,17-31
Immunohistochemistry-Frozen Sections – Reported in literature 2-8
Immunohistochemistry-Paraffin Sections – Reported in literature 9-10
Immunocytochemistry – Reported in literature 32
Immunoprecipitation – Reported in literature 1
Separation – Reported in literature 11-14
Blocking – Reported in literature 15,16
Complement Mediated Cell Depletion – Reported in literature 1

RRID Number AB_2796023
Gene ID 404704 (Porcine)
Gene ID Symbol CD4 (Porcine)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
Email technical support
or call 800.722.2255

Other Conjugates and Formulations

Citations

  1. 1. Pescovitz MD, Lunney JK, Sachs DH. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J Immunol. 1984;133:368-375. (Immunogen, FC, IP, CMDC)
  2. 2. Minguez I, Rueda A, Domínguez J, Sánchez-Vizcaíno JM. Double labeling immunohistological study of African swine fever virus-infected spleen and lymph nodes. Vet Pathol. 1988;25:193-8. (IHC-FS)
  3. 3. Yamada K, Shimizu A, Ierino FL, Utsugi R, Barth RN, Esnaola N, et al. Thymic transplantation in miniature swine. I. Development and function of the "thymokidney". Transplantation. 1999;68:1684-92. (IHC-FS)
  4. 4. Shimizu A, Yamada K, Meehan SM, Sachs DH, Colvin RB. Acceptance reaction: intragraft events associated with tolerance to renal allografts in miniature swine. J Am Soc Nephrol. 2000;11:2371-80. (IHC-FS)
  5. 5. Shimizu A, Yamada K, Sachs DH, Colvin RB. Mechanisms of chronic renal allograft rejection. II. Progressive allograft glomerulopathy in miniature swine. Lab Invest. 2002;82:673-85. (IHC-FS)
  6. 6. Jamin A, Gorin S, Le Potier M, Kuntz-Simon G. Characterization of conventional and plasmacytoid dendritic cells in swine secondary lymphoid organs and blood. Vet Immunol Immunopathol. 2006;114:224-37. (FC, IHC-FS)
  7. 7. Jamin A, Gorin S, Cariolet R, Le Potier M, Kuntz-Simon G. Classical swine fever virus induces activation of plasmacytoid and conventional dendritic cells in tonsil, blood, and spleen of infected pigs. Vet Res. 2008;39:7. (FC, IHC-FS)
  8. 8. Debeer S, Le Luduec J, Kaiserlian D, Laurent P, Nicolas J, Dubois B, et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dematol. 2013;23:456-66. (IHC-FS)
  9. 9. Tingstedt J, Nielsen J. Cellular immune responses in the lungs of pigs infected in utero with PRRSV: an immunohistochemical study. Viral Immunol. 2004;17:558-64. (IHC-PS)
  10. 10. Jung K, Alekseev KP, Zhang X, Cheon D, Vlasova AN, Saif LJ. Altered pathogenesis of porcine respiratory coronavirus in pigs due to immunosuppressive effects of dexamethasone: implications for corticosteroid use in treatment of severe acute respiratory syndrome coronavirus. J Virol. 2007;81:13681-93. (IHC-PS)
  11. 11. Toka FN, Nfon CK, Dawson H, Golde WT. Accessory-cell-mediated activation of porcine NK cells by toll-like receptor 7 (TLR7) and TLR8 agonists. Clin Vaccine Immunol. 2009;16:866-78. (Sep)
  12. 12. Toka FN, Nfon CK, Dawson H, Estes DM, Golde WT. Activation of porcine natural killer cells and lysis of foot-and-mouth disease virus infected cells. J Interferon Cytokine Res. 2009;29:179-92. (Sep)
  13. 13. Toka FN, Nfon C, Dawson H, Golde WT. Natural killer cell dysfunction during acute infection with foot-and-mouth disease virus. Clin Vaccine Immunol. 2009;16:1738-49. (Sep)
  14. 14. Kreuzer S, Rieger J, Strucken EM, Thaben N, Hünigen H, Nöckler K, et al. Characterization of CD4+ subpopulations and CD25+ cells in ileal lymphatic tissue of weaned piglets infected with Salmonella Typhimurium with or without Enterococus faecium feeding. Vet Immunol Immunopathol. 2014;158:143-55. (FC, Sep)
  15. 15. Pescovitz MD, Lunney JK, Sachs DH. Murine anti-swine T4 and T8 monoclonal antibodies: distribution and effects on proliferative and cytotoxic T cells. J Immunol. 1985;134:37-44. (Block, FC)
  16. 16. Saíz JC, Rodríguez A, González M, Alonso F, Sobrino F. Heterotypic lymphoproliferative response in pigs vaccinated with foot-and-mouth disease virus. Involvement of isolated capsid proteins. J Gen Virol. 1992;73:2601-7. (Block)
  17. 17. Davis ME, Maxwell CV, Erf GF, Brown DC, Wistuba TJ. Dietary supplementation with phosphorylated mannans improves growth response and modulates immune function of weanling pigs. J Anim Sci. 2004;82:1882-91. (FC)
  18. 18. Layton DS, Bean AG, Dodge NM, Strom AD, Sandrin MS, Ierino FL. Differential cytokine expression and regulation of human anti-pig xenogeneic responses by modified porcine dendritic cells. Xenotransplantation. 2008;15:257-67. (FC)
  19. 19. Martelli P, Gozio S, Ferrari L, Rosina S, De Angelis E, Quintavalla C, et al. Efficacy of a modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in pigs naturally exposed to a heterologous European (Italian cluster) field strain: Clinical protection and cell-mediated immunity. Vaccine. 2009;27:3788-99. (FC)
  20. 20. Ferrari L, Borghetti P, Gozio S, De Angelis E, Ballotta L, Smeets J, et al. Evaluation of the immune response induced by intradermal vaccination by using a needle-less system in comparison with the intramuscular route in conventional pigs. Res Vet Sci. 2011;90:64-71. (FC)
  21. 21. Oh Y, Seo HW, Han K, Park C, Chae C. Protective effect of the maternally derived porcine circovirus type 2 (PCV2)-specific cellular immune response in piglets by dam vaccination against PCV2 challenge. J Gen Virol. 2012;93:1556-62. (FC)
  22. 22. Ravlo K, Koefoed-Nielsen P, Secher N, Søndergaard P, Keller AK, Petersen MS, et al. Effect of remote ischemic conditioning on dendritic cell number in blood after renal transplantation - flow cytometry in a porcine model. Transpl Immunol. 2012;26:146-50. (FC)
  23. 23. Hester SN, Comstock SS, Thorum SC, Monaco MH, Pence BD, Woods JA, et al. Intestinal and systemic immune development and response to vaccination are unaffected by dietary (1,3/1,6)-β-D-glucan supplementation in neonatal piglets. Clin Vaccine Immunol. 2012;19:1499-508. (FC)
  24. 24. Villena J, Suzuki R, Fujie H, Chiba E, Takahashi T, Tomosada Y, et al. Immunobiotic Lactobacillus jensenii modulates the Toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen-presenting cells. Clin Vaccine Immunol. 2012;19:1038-53. (FC)
  25. 25. Liu Y, Che TM, Song M, Lee JJ, Almeida JA, Bravo D, et al. Dietary plant extracts improve immune responses and growth efficiency of pigs experimentally infected with porcine reproductive and respiratory syndrome virus. J Anim Sci. 2013;91:5668-79. (FC)
  26. 26. Liu KY, Comstock SS, Shunk JM, Monaco MH, Donovan SM. Natural killer cell populations and cytotoxic activity in pigs fed mother's milk, formula, or formula supplemented with bovine lactoferrin. Pediatr Res. 2013;74:402-7. (FC)
  27. 27. Thorum SC, Hester SN, Comstock SS, Monaco MH, Pence BD, Woods JA, et al. Dietary (1,3/1,6)-β-D-glucan decreases transforming growth factor β expression in the lung of the neonatal piglet. Nutr Res. 2013;33:322-31 (FC)
  28. 28. Liu P, Pieper R, Tedin L, Martin L, Meyer W, Rieger J, et al. Effect of dietary zinc oxide on jejunal morphological and immunological characteristics in weaned piglets. J Anim S. 2014;92:5009-18. (FC)
  29. 29. Ferrari L, Borghetti P, De Angelis E, Martelli P. Memory T cell proliferative responses and IFN-γ productivity sustain long-lasting efficacy of a Cap-based PCV2 vaccine upon PCV2 natural infection and associated disease. Vet Res. 2014;45:44. (FC)
  30. 30. Leclercq C, Prunier A, Thomas F, Merlot E. Neonatal surgical castration of male pigs reduces thymic growth but has moderate consequences on thymocytes. J Anim Sci. 2014;92:2415-21. (FC)
  31. 31. Whelan RA, Rausch S, Ebner F, Günzel D, Richter JF, Hering NA, et al. A transgenic probiotic secreting a parasite immunomodulator for site-directed treatment of gut inflammation. Mol Ther. 2014;22:1730-40. (FC)
  32. 32. Matsubara T, Nishii N, Takashima S, Takasu M, Imaeda N, Aiki-Oshima K, et al. Identification and characterization of two CD4 alleles in Microminipigs. BMC Vet Res. 2016;12:222. (ICC)
See All References

Related Products

Related Products

Related Products
Check items to add to the cart or
  1. ELISA plate was coated with serially diluted Mouse IgG<sub>2b</sub>-UNLB (SB Cat. No. 0104-01). Immunoglobulin was detected with Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-BIOT (SB Cat. No. 1080-08), Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG<sub>3</sub>, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Mouse IgG2b-UNLB (A-1)
    0104-01
    $237.00
  2. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-AF647 (SB Cat. No. 1090-31).
    Goat Anti-Mouse IgG2b, Human ads-AF647
    1090-31
    $292.00
  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Fluoromount-G®
    0100-01
    $45.00
  4. Mouse Anti-Porcine Monocyte/Granulocyte-UNLB (74-22-15)
    Mouse Anti-Porcine Monocyte/Granulocyte-UNLB (74-22-15)
    4525-01
    $369.00
  5. Porcine peripheral blood lymphocytes were stained with Mouse Anti-Porcine CD8α-UNLB (SB Cat. No. 4520-01) and Mouse Anti-Porcine CD4-PE (SB Cat. No. 4515-09) followed by Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-FITC (SB Cat. No. 1080-02).
    Mouse Anti-Porcine CD8α-UNLB (76-2-11)
    4520-01
    $369.00
  6. Paraffin embedded minipig vagina section post inoculation with C. trachomatis was stained with Mouse Anti-Porcine CD3ε-UNLB (SB Cat. No. 4510-01) followed by a secondary antibody, DAB, and hematoxylin.<br/>Image from Erneholm K, Lorenzen E, Bøje S, Olsen AW, Andersen P, Cassidy JP, et al. Genital tract lesions in sexually mature Göttingen minipigs during the initial stages of experimental vaginal infection with <i>Chlamydia trachomatis</i> serovar D. BMC Vet Res. 2016;12:200. Figure 2(b)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Mouse Anti-Porcine CD3ε-UNLB (PPT3)
    4510-01
    $303.00
  7. Porcine peripheral blood lymphocytes were stained with Mouse Anti-Porcine CD21-UNLB (SB Cat. No. 4530-01) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-FITC (SB Cat. No. 1070-02).
    Mouse Anti-Porcine CD21-UNLB (BB6-11C9.6)
    4530-01
    $369.00
  8. Mouse Anti-Porcine CD3ε-UNLB (BB23-8E6)
    Mouse Anti-Porcine CD3ε-UNLB (BB23-8E6)
    4511-01
    $303.00
  9. Porcine peripheral blood lymphocytes were stained with Mouse Anti-Porcine CD1-UNLB (SB Cat. No. 4500-01) and Mouse Anti-Porcine CD3ε-PE (SB Cat. No. 4510-09) followed by Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-FITC (SB Cat. No. 1080-02).
    Mouse Anti-Porcine CD1-UNLB (76-7-4)
    4500-01
    $369.00
  10. ELISA plate was coated with purified porcine IgG. Immunoglobulin was detected with Goat Anti-Porcine IgG(H+L)-UNLB (SB Cat. No. 6050-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
    Goat Anti-Porcine IgG(H+L)-UNLB
    6050-01
    $44.00
  11. Frozen section of lamina fibrosa from porcine aortic valve leaflet subjected to bicuspid aortic value wall shear stress was stained with Rat Anti-Mouse CD54-UNLB (SB Cat. No. 1701-01) followed by a secondary antibody and DAPI.<br/>Image from Sun L, Chandra S, Sucosky P. Ex vivo evidence for the contribution of hemodynamic shear stress abnormalities to the early pathogenesis of calcific bicuspid aortic valve disease. PLoS One. 2012;7(10):e48843. Figure 5<br/>Reproduced under the Creative Commons Attribution License
    Rat Anti-Mouse CD54-UNLB (YN1/1.7.4)
    1701-01
    $191.00
  12. Mouse Anti-Human CD54-UNLB (15.2)
    Mouse Anti-Human CD54-UNLB (15.2)
    9441-01
    $103.00
  13. Mouse Anti-Human CD56-UNLB (MEM 188)
    Mouse Anti-Human CD56-UNLB (MEM 188)
    9456-01
    $108.00
  14. Mouse Anti-Human CD62E/CD62P-UNLB (1.2B6)
    Mouse Anti-Human CD62E/CD62P-UNLB (1.2B6)
    9500-01
    $108.00
  15. TNFα stimulated human endothelial cell line HUV-EC-C was stained with Mouse Anti-Human CD106-UNLB (SB Cat. No. 9510-01) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-FITC (SB Cat. No. 1070-02).
    Mouse Anti-Human CD106-UNLB (1.G11B1)
    9510-01
    $92.00
  16. Mouse Anti-Human CD11b-UNLB (ICRF44)
    Mouse Anti-Human CD11b-UNLB (ICRF44)
    9546-01
    $103.00