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Mouse Anti-Mouse H-2Dk-BIOT (15-5-5)
Cat. No.:
1914-08
Biotin Anti-Mouse H-2Dk antibody for use in flow cytometry, ELISA, and immunoprecipitation assays.
$303.00
| Clone | 15-5-5 |
|---|---|
| Isotype | Mouse (C3H.SW) IgG2aκ |
| Isotype Control | Mouse IgG2a-BIOT (HOPC-1) |
| Specificity | Mouse H-2Dk |
| Alternative Names | MHC Class I |
| Description | The monoclonal antibody 15-5-5 reacts with the H-2Dk class I alloantigen. It cross-reacts with H-2Kd and with cells from mice with the H-2f haplotype. The antibody does not react with other (e.g., a, b, p, q, r, s) haplotypes. |
| Immunogen | C3H mouse splenocytes |
| Conjugate | BIOT (Biotin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications |
Flow Cytometry – Quality tested 6 Immunoprecipitation – Reported in literature 3 Complement Mediated Cell Depletion – Reported in literature 1,2 ELISA – Reported in literature 4 Blocking – Reported in literature 5 |
| RRID Number | AB_2795512 |
| Gene ID |
14964 (Mouse) |
| Gene ID Symbol |
H2-D1 (Mouse) |
| Gene ID Aliases | H-2D; H2-D; H2-K1 |
| UniProt ID |
P14426 (Mouse |
| UniProt Name |
HA13_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980;124:533-40. (Immunogen, CMDC)
- 2. Rogers MJ, Gooding LR, Margulies DH, Evans GA. Analysis of a defect in the H-2 genes of SV40 transformed C3H fibroblasts that do not express H-2Kk. J Immunol. 1983;130:2418-22. (CMDC)
- 3. Houlden BA, Widacki SM, Bluestone JA. Signal transduction through class I MHC by a monoclonal antibody that detects multiple murine and human class I molecules. J Immunol. 1991;146:425-30. (IP)
- 4. Bedford P, Garner K, Knight SC. MHC class II molecules transferred between allogeneic dendritic cells stimulate primary mixed leukocyte reactions. Int Immunol. 1999;11:1739-44. (ELISA)
- 5. Desrosiers M, Kielczewska A, Loredo-Osti J, Adam SG, Makrigiannis AP, Lemieux S, et al. Epistasis between mouse Klra and major histocompatibility complex class I loci is associated with a new mechanism of natural killer cell-mediated innate resistance to cytomegalovirus infection. Nat Genet. 2005;37:593-9. (Block)
- 6. Matsuoka S, Asano Y, Sano K, Kishimoto H, Yamashita I, Yorifuji H, et al. A novel type of cell death of lymphocytes induced by a monoclonal antibody without participation of complement. J Exp Med. 1995;181:2007-15. (FC)
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