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Mouse Anti-Mouse H-2Db-LE/AF (27-11-13S)
Cat. No.:
1913-14
Low Endotoxin/Azide Free Anti-Mouse H-2Db antibody for use in flow cytometry, immunocytochemistry, immunoprecipitation, and CMCD assays.
$358.00
| Clone | 27-11-13S |
|---|---|
| Isotype | Mouse (C3H) IgG2aκ |
| Isotype Control | Mouse IgG2a-LE/AF (HOPC-1) |
| Specificity | Mouse H-2Db |
| Alternative Names | MHC Class I |
| Description | The monoclonal antibody 27-11-13S recognizes with the α3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the α3 domain of H-2Dd, H-2Dq, H-2Ld and H-2Lq but not H-2Kb. Reactivity with haplotypes k, f, p, r, and s has not been observed. |
| Immunogen | BDF1 mouse splenocytes |
| Conjugate | LE/AF (Low Endotoxin/Azide Free) |
| Buffer Formulation | Phosphate buffered saline, pH 7.4 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Handle under aseptic conditions |
| Applications |
Flow Cytometry – Quality tested 2 Immunocytochemistry – Reported in literature 3 Immunoprecipitation – Reported in literature 4 Complement Mediated Cell Depletion – Reported in literature 1 |
| RRID Number | AB_2795508 |
| Gene ID |
14964 (Mouse) |
| Gene ID Symbol |
H2-D1 (Mouse) |
| Gene ID Aliases | H-2D; H2-D; H2-K1 |
| UniProt ID |
P01899 (Mouse |
| UniProt Name |
HA11_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 1981;126:317-21. (Immunogen, CMDC)
- 2. Palmowski MJ, Parker M, Choudhuri K, Chiu C, Callan MF, van der Merwe PA. A single-chain H-2Db molecule presenting an influenza virus nucleoprotein epitope shows enhanced ability at stimulating CD8+ T cell responses in vivo. J Immuno. 2009;182:4565-71. (FC)
- 3. Choudhuri K, Parker M, Milicic A, Cole DK, Shaw MK, Sewell AK, et al. Peptide-major histocompatibility complex dimensions control proximal kinase-phosphatase balance during T cell activation. J Biol Chem. 2009;284:26096-105. (ICC)
- 4. Shemesh J, Ehrlich R. Aberrant biosynthesis and transport of class I major histocompatibility complex molecules in cells transformed with highly oncogenic human adenoviruses. J Biol Chem. 1993;268:15704-11. (IP)
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