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Mouse Anti-Mouse CD19-BIOT (MB19-1)
Cat. No.:
1576-08
Biotin Anti-Mouse CD19 antibody for use in flow cytometry and immunoprecipitation assays.
$248.00
| Clone | MB19-1 |
|---|---|
| Isotype | Mouse (129 x C57BL/6) IgAκ |
| Isotype Control | Mouse IgA-BIOT (S107) |
| Specificity | Mouse CD19 |
| Alternative Names | B4, Leu-12 |
| Description | CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. |
| Immunogen | CD19+ mouse pre-B cell line 300.19 |
| Conjugate | BIOT (Biotin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications | Flow Cytometry – Quality tested 1,6 Immunoprecipitation – Reported in literature 1-3,7 Activation – Reported in literature 4,5,7 |
| RRID Number | AB_2794965 |
| Gene ID | 12478 (Mouse) |
| Gene ID Symbol | Cd19 (Mouse) |
| Gene ID Aliases | AW495831 |
| UniProt ID | P25918 (Mouse |
| UniProt Name | CD19_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Sato S, Ono N, Steeber DA, Pisetsky DS, Tedder TF. CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity. J Immunol. 1996;157:4371-8. (Immunogen, FC, IP)
- 2. Yazawa N, Fujimoto M, Sato S, Miyake K, Asano N, Nagai Y, et al. CD19 regulates innate immunity by the toll-like receptor RP105 signaling in B lymphocytes. Blood. 2003;102:1374-80. (IP)
- 3. Fujimoto M, Poe JC, Jansen PJ, Sato S, Tedder TF. CD19 amplifies B lymphocyte signal transduction by regulating Src-family protein tyrosine kinase activation. J Immunol. 1999;162:7088-94. (IP)
- 4. Fujimoto M, Poe JC, Hasegawa M, Tedder TF. CD19 amplification of B lymphocyte Ca2+ responses: a role for Lyn sequestration in extinguishing negative regulation. J Biol Chem. 2001;276:44820-7. (Activ)
- 5. Sato S, Jansen PJ, Tedder TF. CD19 and CD22 expression reciprocally regulates tyrosine phosphorylation of Vav protein during B lymphocyte signaling. Proc Natl Acad Sci USA. 1997;94:13158-62. (Activ)
- 6. Sato S, Steeber DA, Jansen PJ, Tedder TF. CD19 expression levels regulate B lymphocyte development: human CD19 restores normal function in mice lacking endogenous CD19. J Immunol. 1997;158:4662-9. (FC)
- 7. Lee Y, Haas KM, Gor DO, Ding X, Karp DR, Greenspan NS, et al. Complement component C3d-antigen complexes can either augment or inhibit B lymphocyte activation and humoral immunity in mice depending on the degree of CD21/CD19 complex engagement. J Immunol. 2005;175:8011-23. (FC, IP, Activ)
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