Mouse Anti-Influenza A, Nucleoprotein-UNLB (FluA-NP 4F1)
Cat. No.:
10780-01
Purified Anti-Influenza A, Nucleoprotein antibody for use in immunocytochemistry, ELISA, and western blot assays.
$204.00
![Recombinant influenza proteins were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Influenza A, Nucleoprotein-UNLB (SB Cat. No. 10780-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) and chemiluminescent detection.](https://www.southernbiotech.com/media/catalog/product/cache/7149a3d30fe6da616557c86e375dcb18/6/3/63e90fd5805262f2b4baa042dc2e040403d8185ccd8227c0318ae1fdd465c31c.jpeg)
![Recombinant influenza proteins were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Influenza A, Nucleoprotein-UNLB (SB Cat. No. 10780-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) and chemiluminescent detection.](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/6/3/63e90fd5805262f2b4baa042dc2e040403d8185ccd8227c0318ae1fdd465c31c.jpeg)
![A549 cells infected with influenza H1N1 strain NC2 8 hours post infection were stained with Mouse Anti-Influenza A, Nucleoprotein-UNLB (SB Cat. No. 10780-01) followed by an AF555 conjugated secondary antibody and DAPI.<br/>Image from Farooqui A, Leon AJ, Lei Y, Wang P, Huang J, Tenorio R, et al. Heterogeneous virulence of pandemic 2009 influenza H1N1 virus in mice. Virol J. 2012;9:104. Figure 1(c)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/2.0/](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/0/9/09728c92559277e88b6a333d6a397f2c621eefa5a97d8d0a11973a76483aad55.jpeg)
![MDCK cells infected with the influenza A (H1N1) virus were stained with Mouse Anti-Influenza A, Nucleoprotein-UNLB (SB Cat. No. 10780-01) followed by a secondary antibody and DAPI.<br/>Image from Shoji M, Woo S, Masuda A, Win NN, Ngwe H, Takahashi E, et al. Anti-influenza virus activity of extracts from the stems of <i>Jatropha multifida</i> Linn. collected in Myanmar. BMC Complement Altern Med. 2017;17:96. Figure 3(a)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/9/5/954f10ffad76de354adcfdf5a49cc4a9fae910c214ab62807b41943453bba598.jpeg)
Clone | FluA-NP 4F1 |
---|---|
Isotype | Mouse (BALB/c) IgG1κ |
Isotype Control | Mouse IgG1-UNLB (15H6) |
Specificity | Influenza virus type A nucleoprotein |
Alternative Names | NP |
Description | Influenza virus type A nucleoprotein, also known as NP, is composed of a 498 amino acid sequence and is type-specific in influenza viruses. The NP encapsulates the virus genome to form a ribonucleoprotein (RNP) particle for the purposes of transcription and packaging. The monoclonal antibody FluA-NP 4F1 has been shown to specifically recognize type A influenza virus NP in virus infected cell culture and patient samples. This clone can be paired as a detection antibody for ELISA with clone FluA-NP 2C9 (SB Cat. No. 10770-01). |
Immunogen | Recombinant influenza virus type A nucleoprotein |
Conjugate | UNLB (Unconjugated) |
Buffer Formulation | Borate buffered saline, pH 8.2 |
Clonality | Monoclonal |
Concentration | 0.5 mg/mL |
Volume | 1.0 mL |
Recommended Storage | 2-8°C |
Applications |
ELISA – Quality tested Immunocytochemistry – Reported in literature 1-4,6,7 Western Blot – Reported in literature 2,4,5 |
RRID Number | AB_2794457 |
Documentation
Certificate of Analysis Lookup
Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view
- 1. Wei G, Meng W, Guo H, Pan W, Liu J, Peng T, et al. Potent neutralization of influenza A virus by a single-domain antibody blocking M2 ion channel protein. PLoS One. 2011;6(12):e28309. (ICC)
- 2. Shoji M, Takahashi E, Hatakeyama D, Iwai Y, Morita Y, Shirayama R, et al. Anti-influenza activity of C60 fullerene derivatives. PLoS One. 2013;8(6):e66337. (ICC, WB)
- 3. Hatakeyama D, Shoji M, Yamayoshi S, Hirota T, Nagae M, Yanagisawa S, et al. A novel functional site in the PB2 subunit of influenza A virus essential for acetyl-CoA interaction, RNA polymerase activity, and viral replication. J Biol Chem. 2014;289:24980-94. (ICC)
- 4. Shoji M, Arakaki Y, Esumi T, Kohnomi S, Yamamoto C, Suzuki Y, et al. Bakuchiol is a phenolic isoprenoid with novel enantiomer-selective anti-influenza A virus activity involving Nrf2 activation. J Biol Chem. 2015 Oct 7. pii: jbc.M115.669465. [Epub ahead of print]. (ICC, WB)
- 5. Narkpuk J, Wanitchang A, Kramyu J, Frantz PN, Jongkaewwattana A, Teeravechyan S. An unconventional BST-2 function: down-regulation of transient protein expression. Biochem Biophys Res Commun. 2014;450:1469-74. (WB)
- 6. Farooqui A, Leon AJ, Lei Y, Wang P, Huang J, Tenorio R, et al. Heterogeneous virulence of pandemic 2009 influenza H1N1 virus in mice. Virol J. 2012;9:104. (ICC)
- 7. Shoji M, Woo S, Masuda A, Win NN, Ngwe H, Takahashi E, et al. Anti-influenza virus activity of extracts from the stems of Jatropha multifida Linn. collected in Myanmar. BMC Complement Altern Med. 2017;17:96. (ICC)
See All References