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  • Mouse Anti-Human Lambda-UNLB (JDC-12)

Mouse Anti-Human Lambda-UNLB (JDC-12)

Cat. No.:
9180-01
Purified Anti-Human Lambda antibody for use in ELISA, flow cytometry, western blot, and immunoprecipitation assays.
$143.00
Size Price (USD) Quantity
0.5 mg $143.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone JDC-12
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-UNLB (15H6)
Specificity Human/Rhesus Lambda
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested 1,2
FLISA – Quality tested
Flow Cytometry – Quality tested 4,5
Western Blot – Reported in literature 3
Immunoprecipitation – Reported in literature 4

RRID Number AB_2796674
Gene ID 3535 (Human)
Gene ID Symbol IGL (Human)
Gene ID Aliases IGL@; IGLC6

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Du C, Wingard JR, Cheng S, Nguyen MH, Clancy CJ. Serum IgG responses against Aspergillus proteins before hematopoietic stem cell transplantation or chemotherapy identify patients who develop invasive aspergillosis. Biol Blood Marrow Transplant. 2012;18:1927-34. (ELISA)
  2. 2. Einarsdottir HK, Stapleton NM, Scherjon S, Andersen JT, Rispens T, van der Schoot CE, et al. On the perplexingly low rate of transport of IgG2 across the human placenta. PLoS One. 2014;9(9):e108319. (ELISA)
  3. 3. Dobbs AK, Yang T, Farmer D, Kager L, Parolini O, Conley ME. Cutting edge: a hypomorphic mutation in Igβ (CD79b) in a patient with immunodeficiency and a leaky defect in B cell development. J Immunol. 2007;179:2055-9. (WB)
  4. 4. Pauza ME, Rehmann JA, LeBien TW. Unusual patterns of immunoglobulin gene rearrangement and expression during human B cell ontogeny: human B cells can simultaneously express cell surface κ and λ light chains. J Exp Med. 1993;178:139-49. (IP, FC)
  5. 5. Stiernholm NB, Berinstein NL. A mutated promoter of a human Ig Vλ gene segment is associated with reduced germ-line transcription and a low frequency of rearrangement. J Immuol. 1995;154:1748-61. (FC)
  6. 6. Nonhuman Primate Reagent Resource (Rhesus Reactivity)
See All References

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  2. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa-UNLB (SB Cat. No. 2060-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  4. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Mouse Anti-Human Kappa-UNLB (SB Cat. No. 9230-01) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-HRP (SB Cat. No. 1070-05).
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  5. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human ads-AF647 (SB Cat. No. 1070-31).
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  6. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-UNLB (SB Cat. No. 2070-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  7. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Kappa-UNLB (SB Cat. No. 0151K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  8. CD19+ human B-lymphocytes were stained with Goat Anti-Human Kappa, Mouse ads-UNLB (SB Cat. 2061-01) and Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-PE (SB Cat. No. 2073-09) followed by Mouse Anti-Goat IgG Fc-AF488 (SB Cat. No. 6158-30).
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