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  • Mouse Anti-Human Lambda-BIOT (JDC-12-R - Recombinant)

Mouse Anti-Human Lambda-BIOT (JDC-12-R - Recombinant)

Cat. No.:
29180-08
Biotin Anti-Human Lambda recombinant antibody for use in ELISA and flow cytometry assays.
$115.00
Size Price (USD) Quantity
0.25 mg $115.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone JDC-12-R
(Genetically derived from DNA sequences of a parental hybridoma clone; for details on the parental clone, see Cat. No. 9180)
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-BIOT (15H6)
Specificity Human Lambda
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.5 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested
Flow Cytometry – Quality tested

RRID Number AB_3076635
Gene ID 3535 (Human)
Gene ID Symbol IGL (Human)
Gene ID Aliases IGL@; IGLC6

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with Goat Anti-Mouse IgG<sub>1</sub>, Human ads-UNLB (SB Cat. No. 1070-01), Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-UNLB (SB Cat. No. 1080-01), Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-UNLB (SB Cat. No. 1090-01), Goat Anti-Mouse IgG<sub>3</sub>, Human ads-UNLB (SB Cat. No. 1100-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgG<sub>1</sub>-BIOT (SB Cat. No. 0102-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  2. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  3. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  4. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-BIOT (SB Cat. No. 2070-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  6. FLISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-PE (SB Cat. No. 7105-09S).
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  7. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa-BIOT (SB Cat. No. 2060-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  8. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Lambda-UNLB (SB Cat. No. 0151L-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  9. CD19+ human B-lymphocytes were stained with Goat Anti-Human Lambda, Mouse ads-BIOT (SB Cat. 2071-08) and Goat F(ab')<sub>2</sub> Anti-Human Kappa, Mouse ads-PE (SB Cat. No. 2063-09) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
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  10. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa, Mouse ads-BIOT (SB Cat. No. 2061-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  11. Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (SB Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05) secondary antibody and chemiluminescent detection.
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  12. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Kappa-UNLB (SB Cat. No. 0151K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  13. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Lambda-BIOT (SB Cat. No. 2072-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  14. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Kappa-BIOT (SB Cat. No. 2062-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  15. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-BIOT (SB Cat. No. 2073-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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