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Mouse Anti-Human IgG3 Hinge-BIOT (HP6050)

Mouse Anti-Human IgG3 Hinge-BIOT (HP6050)

Name: Mouse Anti-Human IgG3 Hinge-BIOT (HP6050)
SKU: 9210-08
Price: 193 USD
Availability: InStock

Cat. No.:

9210-08

Biotin Anti-Human IgG₃ Hinge antibody for use in ELISA, flow cytometry, immunohistochemistry, western blot, immunoprecipitation, ELISpot, and multiplex assays.

$193.00

Size	Price (USD)	Quantity
0.5 mg	$193.00

Qty

Technical Bulletin Safety Datasheet

ELISA plate was coated with purified human IgG<sub>1</sub>, IgG<sub>2</sub>, IgG<sub>3</sub>, and IgG<sub>4</sub>. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08) followed by Streptavi

ELISA plate was coated with purified human IgG₁, IgG₂, IgG₃, and IgG₄. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG₃ Hinge-BIOT (SB Cat. No. 9210-08) followed by Streptavi

Product Specifications

More Information
Clone	HP6050
Isotype	Mouse (BALB/c) IgG₁κ
Isotype Control	Mouse IgG1-BIOT (15H6)
Specificity	Human IgG₃ Hinge
Immunogen	Human IgG₃ myeloma protein
Conjugate	BIOT (Biotin)
Buffer Formulation	Phosphate buffered saline containing < 0.1% sodium azide
Clonality	Monoclonal
Concentration	0.5 mg/mL
Volume	1.0 mL
Recommended Storage	2-8°C
Applications	ELISA – Quality tested ^2-10,14,16 FLISA – Quality tested ELISpot – Reported in literature ¹⁰ Flow Cytometry – Reported in literature ^17,18 Immunohistochemistry-Frozen Sections – Reported in literature ^11,12 Immunohistochemistry-Paraffin Sections – Reported in literature ^13,14 Western Blot – Reported in literature ^15,16 Immunoprecipitation – Reported in literature ¹ Multiplex – Reported in literature ^19-21 Purification – Reported in literature ³ Surface Plasmon Resonance – Reported in literature ²²
RRID Number	AB_2796700
Gene ID	3502 (Human)
Gene ID Symbol	IGHG3 (Human)
Gene ID Aliases	IgG3
UniProt ID	P01860 (Human
UniProt Name	IGHG3_HUMAN (Human)

Documentation

Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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Other Conjugates and Formulations

Citations

1. Reimer CB, Phillips DJ, Aloisio CH, Moore DD, Galland GG, Wells TW, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma. 1984;3:263-75. (Immunogen, IP)
2. Tomana M, Novak J, Julian BA, Matousovic K, Konecny K, Mestecky J. Circulating immune complexes in IgA nephropathy consist of IgA1 with galactose-deficient hinge region and antiglycan antibodies. J Clin Invest. 1999;104:73-81. (ELISA)
3. Kobayashi T, van der Pol W, van de Winkel JG, Hara K, Sugita N, Westerdaal NA, et al. Relevance of IgG receptor IIIb (CD16) polymorphism to handling of Porphyromonas gingivalis: implications for the pathogenesis of adult periodontitis. J Periodontal Res. 2000;35:65-73. (ELISA, Purification)
4. Xu P, Cui Z, Chen M, Hellmark T, Zhao M. Comparison of characteristics of natural autoantibodies against myeloperoxidase and anti-myeloperoxidase autoantibodies from patients with microscopic polyangiitis. Rheumatology. 2011;50:1236-43. (ELISA)
5. Geisler WM, Morrison SG, Doemland ML, Iqbal SM, Su J, Mancevski A, et al. Immunoglobulin-specific responses to Chlamydia elementary bodies in individuals with and at risk for genital chlamydial infection. J Infect Dis. 2012;206:1836-43. (ELISA)
6. Sabbatini P, Tsuji T, Ferran L, Ritter E, Sedrak C, Tuballes K, et al. Phase I trial of overlapping long peptides from a tumor self-antigen and poly-ICLC shows rapid induction of integrated immune response in ovarian cancer patients. Clin Cancer Res. 2012;18:6497-508. (ELISA)
7. Westra J, van Assen S, Wilting KR, Land J, Horst G, de Haan A, et al. Rituximab impairs immunoglobulin (Ig)M and IgG (subclass) responses after influenza vaccination in rheumatoid arthritis patients. Clin Exp Immunol. 2014;178:40-7. (ELISA)
8. Ferreira AR, Singh B, Cabrera-Mora M, Magri De Souza AC, Queiroz Marques MT, Porto LC, et al. Evaluation of naturally acquired IgG antibodies to a chimeric and non-chimeric recombinant species of Plasmodium vivax reticulocyte binding protein-1: lack of association with HLA-DRB1*/DQB1* in malaria exposed individuals from the Brazilian Amazon. PLoS One. 2014;9(8):e105828. (ELISA)
9. Haag S, Schneider N, Mason DE, Tuncel J, Andersson IE, Peters EC, et al. Identification of new citrulline-specific autoantibodies, which bind to human arthritic cartilage, by mass spectrometric analysis of citrullinated type II collagen. Arthritis Rheumatol. 2014;66:1440-9. (ELISA)
10. Næss LM, Aarvak T, Aase A, Oftung F, Høiby EA, Sandin R, et al. Human IgG subclass responses in relation to serum bactericidal and opsonic activities after immunization with three doses of the Norwegian serogroup B meningococcal outer membrane vesicle vaccine. Vaccine. 1999;17:754-64. (ELISA, ELISPOT)
11. Greenlee JE, Boyden JW, Pingree M, Brashear HR, Clawson SA, Keeney PM. Antibody types and IgG subclasses in paraneoplastic neurological syndromes. J Neurol Sci. 2001;184:131-7. (IHC-FS)
12. Lin J, Markowitz GS, Valeri AM, Kambham N, Sherman WH, Appel GB, et al. Renal monoclonal immunoglobulin deposition disease: the disease spectrum. J Am Soc Nephrol. 2001;12:1482-92. (IHC-FS)
13. Qu Z, Liu G, Li J, Wu L, Tan Y, Zheng X, et al. Absence of glomerular IgG4 deposition in patients with membranous nephropathy may indicate malignancy. Nephrol Dial Transplant. 2012;27:1931-7. (IHC-PS)
14. Jia X, Hu S, Chen J, Qu Z, Liu G, Cui Z, et al. The clinical and immunological features of patients with combined anti-glomerular basement membrane disease and membranous nephropathy. Kidney Int. 2014;85:945-52. (ELISA, IHC-PS)
15. Bogdanos D, Pares A, Baum H, Caballeria L, Rigopoulou EI, Ma Y, et al. Disease-specific cross-reactivity between mimicking peptides of heat shock protein of Mycobacterium gordonae and dominant epitope of E2 subunit of pyruvate dehydrogenase is common in Spanish but not British patients with primary biliary cirrhosis. J Autoimmun. 2004;22:353-62. (WB)
16. Bogdanos D, Pusl T, Rust C, Vergani D, Beuers U. Primary biliary cirrhosis following Lactobacillus vaccination for recurrent vaginitis. J Hepatol. 2008;49:466-73. (ELISA, WB)
17. Toyoda C, Suzuki Y, Tsuneyama H, Onodera T, Masuno A, Yabe R, et al. Production of human monoclonal anti-Jk3, recognising an epitope including the Jk^a/Jk^b polymorphic site of the Kidd glycoprotein. Transfus Med. 2014;24:286-91. (FC)
18. Gao B, Moore C, Porcheray F, Rong C, Abidoglu C, DeVito J, et al. Pretransplant IgG reactivity to apoptotic cells correlates with late kidney allograft loss. Am J Transplant. 2014;14:1581-91. (FC)
19. Hönger G, Hopfer H, Arnold M, Spriewald BM, Schaub S, Amico P. Pretransplant IgG subclasses of donor-specific human leukocyte antigen antibodies and development of antibody-mediated rejection. Transplantation. 2011;92:41-7. (Multiplex)
20. Schaub S, Hönger G, Koller MT, Liwski R, Amico P. Determinants of C1q binding in the single antigen bead assay. Transplantation. 2014;98:387-93. (Multiplex)
21. Bentall A, Tyan DB, Sequeria F, Everly MJ, Gandhi MJ, Cornell LD, et al. Antibody-mediated rejection despite inhibition of terminal complement. Transpl Int. 2014;27:1235-43. (Multiplex)
22. Lewis KB, Hughes RJ, Epstein MS, Josephson NC, Kempton CL, Kessler CM, et al. Phenotypes of allo- and autoimmune antibody responses to FVIII characterized by surface plasmon resonance. PLoS One. 2013;8(5):e61120. (Surface Plasmon Resonance)