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Mouse Anti-Human IgG2 Fc-AF555 (HP6002)
Cat. No.:
9070-32
Alexa Fluor® 555 Anti-Human IgG2 Fc antibody for use in immunohistochemistry and western blot assays.
$289.00
| Clone | HP6002 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | 0102-32 |
| Specificity | Human IgG2 Fc |
| Immunogen | Human IgG2 myeloma protein |
| Conjugate | AF555 (Alexa Fluor® 555) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 0.2 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications | ELISA – Quality tested 2-7 FLISA – Quality tested ELISpot – Reported in literature 2,8 Flow Cytometry – Reported in literature 13 Immunohistochemistry-Frozen Sections – Reported in literature 9,10 Western Blot – Reported in literature 9,11,12 Immunoprecipitation – Reported in literature 1 Multiplex – Reported in literature 14,15 Purification – Reported in literature 16 Surface Plasmon Resonance – Reported in literature 17 |
| RRID Number | AB_2796642 |
| Gene ID | 3501 (Human) |
| Gene ID Symbol | IGHG2 (Human) |
| UniProt ID | P01859 (Human |
| UniProt Name | IGHG2_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Reimer CB, Phillips DJ, Aloisio CH, Moore DD, Galland GG, Wells TW, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma. 1984;3:263-75. (Immunogen, IP)
- 2. Næss LM, Aarvak T, Aase A, Oftung F, Høiby EA, Sandin R, et al. Human IgG subclass responses in relation to serum bactericidal and opsonic activities after immunization with three doses of the Norwegian serogroup B meningococcal outer membrane vesicle vaccine. Vaccine. 1999;17:754-64. (ELISA, ELISPOT)
- 3. Kobayashi T, van der Pol W, van de Winkel JG, Hara K, Sugita N, Westerdaal NA, et al. Relevance of IgG receptor IIIb (CD16) polymorphism to handling of Porphyromonas gingivalis: implications for the pathogenesis of adult periodontitis. J Periodontal Res. 2000;35:65-73. (ELISA)
- 4. Lightle S, Aykent S, Lacker N, Mitaksov V, Wells K, Zobel J, et al. Mutations within a human IgG2 antibody form distinct and homogeneous disulfide isomers but do not affect Fc gamma receptor or C1q binding. Protein Sci. 2010;19:753-62. (ELISA)
- 5. Sabbatini P, Tsuji T, Ferran L, Ritter E, Sedrak C, Tuballes K, et al. Phase I trial of overlapping long peptides from a tumor self-antigen and poly-ICLC shows rapid induction of integrated immune response in ovarian cancer patients. Clin Cancer Res. 2012;18:6497-508. (ELISA)
- 6. Einarsdottir HK, Stapleton NM, Scherjon S, Andersen JT, Rispens T, van der Schoot CE, et al. On the perplexingly low rate of transport of IgG2 across the human placenta. PLoS One. 2014;9(9):e108319. (ELISA)
- 7. Ferreira AR, Singh B, Cabrera-Mora M, Magri De Souza AC, Queiroz Marques MT, Porto LC, et al. Evaluation of naturally acquired IgG antibodies to a chimeric and non-chimeric recombinant species of Plasmodium vivax reticulocyte binding protein-1: lack of association with HLA-DRB1*/DQB1* in malaria exposed individuals from the Brazilian Amazon. PLoS One. 2014;9(8):e105828. (ELISA)
- 8. Carson PJ, Schut RL, Simpson ML, O'Brien J, Janoff EN. Antibody class and subclass responses to pneumococcal polysaccharides following immunization of human immunodeficiency virus-infected patients. J Infect Dis. 1995;172:340-5. (ELISPOT)
- 9. Mongan LC, Ockleford CD. Behaviour of two IgG subclasses in transport of immunoglobulin across the human placenta. J Anat. 1996;188:43-51. (IHC-FS, WB)
- 10. Greenlee JE, Boyden JW, Pingree M, Brashear HR, Clawson SA, Keeney PM. Antibody types and IgG subclasses in paraneoplastic neurological syndromes. J Neurol Sci. 2001;184:131-7. (IHC-FS)
- 11. Harada S, Hata S, Kosada Y, Kondo E. Identification of epitopes recognized by a panel of six anti-human IgG2 monoclonal antibodies. J Immunol Methods. 1991;141:89-96. (WB)
- 12. Dibart S, Eftimiadi C, Socransky S, Taubman MA, van Dyke TE. Rapid evaluation of serum and gingival crevicular fluid immunoglobulin G subclass antibody levels in patients with early-onset periodontitis using checkerboard immunoblotting. Oral Microbiol Immunol. 1998;13:166-72. (WB)
- 13. Gao B, Moore C, Porcheray F, Rong C, Abidoglu C, DeVito J, et al. Pretransplant IgG reactivity to apoptotic cells correlates with late kidney allograft loss. Am J Transplant. 2014;14:1581-91. (FC)
- 14. Kaneku H, O'Leary JG, Taniguchi M, Susskind BM, Terasaki PI, Klintmalm GB. Donor-specific human leukocyte antigen antibodies of the immunoglobulin G3 subclass are associated with chronic rejection and graft loss after liver transplantation. Liver Transpl. 2012;18:984-92. (Multiplex)
- 15. Freitas MC, Rebellato LM, Ozawa M, Nguyen A, Sasaki N, Everly M, et al. The role of immunoglobulin-G subclasses and C1q in de novo HLA-DQ donor-specific antibody kidney transplantation outcomes. Transplantation. 2013;95:1113-9. (Multiplex)
- 16. Keen J, Serghides L, Ayi K, Patel SN, Ayisi J, van Eijk A, et al. HIV impairs opsonic phagocytic clearance of pregnancy-associated malaria parasites. PLoS Med. 2007;4(5):e181. (Purification)
- 17. Lewis KB, Hughes RJ, Epstein MS, Josephson NC, Kempton CL, Kessler CM, et al. Phenotypes of allo- and autoimmune antibody responses to FVIII characterized by surface plasmon resonance. PLoS One. 2013;8(5):e61120. (Surface Plasmon Resonance)
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