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Mouse Anti-Human IFN-γ-FITC (B27)
Cat. No.:
10114-02
FITC Anti-Human IFN-gamma antibody for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.
$132.00
| Clone | B27 |
|---|---|
| Isotype | Mouse IgG1κ |
| Isotype Control | Mouse IgG1-FITC (15H6) |
| Specificity | Human/Rhesus/Cynomolgus/Chimpanzee/Pigtail Macaque/African Green Monkey/Sooty Mangabey IFN-γ |
| Alternative Names | Interferon-γ, gIFN, IFNG, immune interferon, IIF, type II interferon, T interferon, T cell interferon, mitogen induced interferon, pH2-labile interferon, macrophage-activating factor, MAF |
| Immunogen | E. coli-expressed human IFN-γ |
| Conjugate | FITC (Fluorescein) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Applications |
ELISA-Detection – Quality tested 1-4,11 Flow Cytometry – Quality tested 9,10,12-18 ELISpot-Capture – Reported in literature 5-10 Immunohistochemistry-Frozen Sections – Reported in literature 19 Immunocytochemistry – Reported in literature 20 Immunoprecipitation – Reported in literature 11 Neutralization – Reported in literature 1,21 Multiplex-Capture – Reported in literature 22 Note – May be paired with the purified clone A35 (SB Cat. No. 10113-01) in a sandwich ELISA |
| RRID Number | AB_2794141 |
| Gene ID |
3458 (Human) 574282 (Rhesus) 102128291 (Chimpanzee) 449517 (Cynomolgus) 105473416 (Pigtail Macaque) 103238673 (Sooty Mangabey) 105600509 (Green Monkey) |
| Gene ID Symbol |
IFNG (Human) IFNG (Rhesus) IFNG (Chimpanzee) IFNG (Cynomolgus) IFNG (Pigtail Macaque) IFNG (Sooty Mangabey) IFNG (Green Monkey) |
| Gene ID Aliases | IFG; IFIl; EGK_03901; IFN-gamma; CK820_G0041509 |
| UniProt ID |
P01579 (Human P63310 (Rhesus Q9TTB0 (Chimpanzee P63309 (Cynomolgus P63311 (Pigtail Macaque P42162 (Sooty Mangabey |
| UniProt Name |
IFNG_HUMAN (Human) IFNG_MACMU (Rhesus) IFNG_MACFA (Chimpanzee) IFNG_PANTR (Cynomolgus) IFNG_MACNE (Pigtail Macaque) IFNG_CERAT (Sooty Mangabey) |
Documentation
Certificate of Analysis Lookup
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- 1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
- 2. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
- 3. Lagier B, Lebel B, Bousquet J, Péne J. Different modulation by histamine of IL-4 and interferon-gamma (IFN-γ) release according to the phenotype of human Th0, Th1 and Th2 clones. Clin Exp Immunol. 1997;108:545-51. (ELISA-Detection)
- 4. Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P. Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression. Am J Pathol. 2004;164:409-17. (ELISA-Detection)
- 5. Barouch DH, Kunstman J, Kuroda MJ, Schmitz JE, Santra S, Peyerl FW, et al. Eventual AIDS vaccine failure in a rhesus monkey by viral escape from cytotoxic T lymphocytes. Nature. 2002;415:335-9. (ELISPOT-Capture, Rhesus Reactivity)
- 6. Malkevitch N, Rohne D, Pinczewski J, Aldrich K, Kalyanaraman VS, Letvin NL, et al. Evaluation of combination DNA/replication-competent Ad-SIV recombinant immunization regimens in rhesus macaques. AIDS Res Hum Retroviruses. 2004;20:235-44. (ELISPOT-Capture, Rhesus Reactivity)
- 7. Shen L, Shen Y, Huang D, Qiu L, Sehgal P, Du GZ, et al. Development of Vγ2Vδ2+ T cell responses during active mycobacterial coinfection of simian immunodeficiency virus-infected macaques requires control of viral infection and immune competence of CD4+ T cells. J Infect Dis. 2004;190:1438-47. (ELISPOT-Capture, Rhesus & Pigtail Macaque Reactivity)
- 8. Reimann KA, Parker RA, Seaman MS, Beaudry K, Beddall M, Peterson L, et al. Pathogenicity of simian-human immunodeficiency virus SHIV-89.6P and SIVmac is attenuated in cynomolgus macaques and associated with early T-lymphocyte responses. J Virol. 2005;79:8878-85. (ELISPOT-Capture, Cynomolgus & Rhesus Reactivity)
- 9. Letvin NL, Rao SS, Dang V, Buzby AP, Korioth-Schmitz B, Dombagoda D, et al. No evidence for consistent virus-specific immunity in simian immunodeficiency virus-exposed, uninfected rhesus monkeys. J Virol. 2007;81:12368-74. (ELISPOT-Capture, FC, Rhesus Reactivity)
- 10. Zahn RC, Rett MD, Korioth-Schmitz B, Sun Y, Buzby AP, Goldstein S, et al. Simian immunodeficiency virus (SIV)-specific CD8+ T-cell responses in vervet African green monkeys chronically infected with SIVagm. J Virol. 2008;82:11577-88. (ELISPOT-Capture, FC, African Green Monkey & Rhesus Reactivity)
- 11. Favre C, Wijdenes J, Cabrillat H, Djossou O, Banchereau J, de Vries JE. Epitope mapping of recombinant human gamma interferon using monoclonal antibodies. Mol Immunol. 1989;26:17-25. (ELISA-Detection, IP)
- 12. Sumpter B, Dunham R, Gordon S, Engram J, Hennessy M, Kinter A, et al. Correlates of preserved CD4+ T cell homeostasis during natural, nonpathogenic simian immunodeficiency virus infection of sooty mangabeys: implications for AIDS pathogenesis. J Immunol. 2007;178:1680-91. (FC, Sooty Mangabey Reactivity)
- 13. De Rose R, Batten CJ, Smith MZ, Fernandez CS, Peut V, Thomson S, et al. Comparative efficacy of subtype AE simian-human immunodeficiency virus priming and boosting vaccines in pigtail macaques. J Virol. 2007;81:292-300. (FC, Pigtail Macaque Reactivity)
- 14. Liu J, Ewald BA, Lynch DM, Denholtz M, Abbink P, Lemckert AA, et al. Magnitude and phenotype of cellular immune responses elicited by recombinant adenovirus vectors and heterologous prime-boost regimens in rhesus monkeys. J Virol. 2008;82:4844-52. (FC, Rhesus Reactivity)
- 15. Mattila JT, Diedrich CR, Lin PL, Phuah J, Flynn JL. Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation. J Immunol. 2011;186:3527-37. (FC, Cynomolgus Reactivity)
- 16. Wilks AB, Perry JR, Ehlinger EP, Zahn RC, White R, Gauduin M, et al. High cell-free virus load and robust autologous humoral immune responses in breast milk of simian immunodeficiency virus-infected african green monkeys. J Virol. 2011;85:9517-26. (FC, African Green Monkey Reactivity)
- 17. Reeves RK, Evans TI, Fultz PN, Johnson RP. Potential confusion of contaminating CD16+ myeloid DCs with anergic CD16+ NK cells in chimpanzees. Eur J Immunol. 2011;41:1070-4. (FC, Chimpanzee Reactivity)
- 18. Lin PL, Rutledge T, Green AM, Bigbee M, Fuhrman C, Klein E, et al. CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques. AIDS Res Hum Retroviruses. 2012;28:1693-702. (FC, Cynomolgus Reactivity)
- 19. Stary G, Klein I, Brüggen M, Kohlhofer S, Brunner PM, Spazierer D, et al. Host defense mechanisms in secondary syphilitic lesions: a role for IFN-γ-/IL-17-producing CD8+ T cells?. Am J Pathol. 2010;177:2421-32. (IHC-FS)
- 20. Chemin K, Bohineust A, Dogniaux S, Tourret M, Guégan S, Miro F, et al. Cytokine secretion by CD4+ T cells at the immunological synapse requires Cdc42-dependent local actin remodeling but not microtubule organizing center polarity. J Immunol. 2012;189:2159-68. (ICC)
- 21. Gangur V, Simons FE, Hayglass KT. Human IP-10 selectively promotes dominance of polyclonally activated and environmental antigen-driven IFN-γ over IL-4 responses. FASEB J. 1998;12:705-13. (Neut)
- 22. Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Capture, Cynomolgus Reactivity)
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