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Mouse Anti-Human CD8-AF700 (RFT8)
Cat. No.:
9536-27
Alexa Fluor® 700 Anti-Human CD8 antibody for use in flow cytometry assays.
$253.00
| Clone | RFT8 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-AF700 (15H6) |
| Specificity | Human CD8 |
| Alternative Names | Leu-2, Lyt2, Lyt3, Tp32, T8 |
| Description | CD8 is a 33 kDa transmembrane glycoprotein expressed as either a CD8αα homodimer or CD8αβ heterodimer. It is expressed on the “cytotoxic/suppressor” subpopulation of peripheral T cells. CD8 functions primarily as a coreceptor with MHC class I-restricted TCR’s in antigen recognition. |
| Immunogen | Thymocytes and E-rosetted lymphocytes |
| Conjugate | AF700 (Alexa Fluor® 700) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications |
Flow Cytometry – Quality tested 1,9 Immunohistochemistry-Frozen Sections – Reported in literature 2-5 Immunohistochemistry-Paraffin Sections – Reported in literature 6 Immunocytochemistry – Reported in literature 7 Depletion – Reported in literature 8 |
| RRID Number | AB_2796894 |
| Gene ID |
925 (Human) |
| Gene ID Symbol |
CD8A (Human) |
| Gene ID Aliases | CD8; p32; Leu2 |
| UniProt ID |
P01732 (Human |
| UniProt Name |
CD8A_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Bofill M, Janossy G, Lee CA, MacDonald-Burns D, Phillips AN, Sabin C, et al. Laboratory control values for CD4 and CD8 T lymphocytes. Implications for HIV-1 diagnosis. Clin Exp Immunol. 1992;88:243-52. (Immunogen, FC)
- 2. Bofill M, Gombert W, Borthwick NJ, Akbar AN, McLaughlin JE, Lee CA, et al. Presence of CD3+CD8+Bcl-2low lymphocytes undergoing apoptosis and activated macrophages in lymph nodes of HIV-1+ patients. Am J Pathol. 1995;146:1542-55. (IHC-FS)
- 3. Gay FW, Drye TJ, Dick GW, Esiri MM. The application of multifactorial cluster analysis in the staging of plaques in early multiple sclerosis. Identification and characterization of the primary demyelinating lesion. Brain. 1997;120:1461-83. (IHC-FS)
- 4. Hufert FT, van Lunzen J, Janossy G, Bertram S, Schmitz J, Haller O, et al. Germinal centre CD4+ T cells are an important site of HIV replication in vivo. AIDS. 1997;11:849-57. (IHC-FS)
- 5. Howie D, Spencer J, DeLord D, Pitzalis C, Wathen NC, Dogan A, et al. Extrathymic T cell differentiation in the human intestine early in life. J Immunol. 1998;161:5862-72. (IHC-FS)
- 6. Cruickshank SM, Southgate J, Wyatt JI, Selby PJ, Trejdosiewicz LK. Expression of CD44 on bile ducts in primary sclerosing cholangitis and primary biliary cirrhosis. J Clin Pathol. 1999;52:730-4. (IHC-PS)
- 7. Burgess R, Hyde K, Maguire PJ, Kelsey PR, Yin JA, Geary CG. Two-colour immunoenzymatic technique using sequential staining by APAAP to evaluate two cell antigens. J Clin Pathol. 1992;45:206-9. (ICC)
- 8. Wimperis JZ, Gottlieb D, Duncombe AS, Heslop HE, Prentice HG, Brenner MK. Requirements for the adoptive transfer of antibody responses to a priming antigen in man. J Immunol. 1990;144:541-7. (Depletion)
- 9. Hunter PJ, Nistala K, Jina N, Eddaoudi A, Thomson W, Hubank M, et al. Biologic predictors of extension of oligoarticular juvenile idiopathic arthritis as determined from synovial fluid cellular composition and gene expression. Arthritis Rheum. 2010;62:896-907. (FC)
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