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Mouse Anti-Human CD74-BIOT (LN2)
Cat. No.:
9775-08
Biotin Anti-Human CD74 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, western blot, and immunoprecipitation assays.
$174.00
| Clone | LN2 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-BIOT (15H6) |
| Specificity | Human CD74 |
| Alternative Names | Invariant chain, Ii, class II-specific chaperone |
| Description | CD74 represents a type II integral membrane protein known as MHC class II-associated invariant chain. It is moderately expressed on B cells and weakly on monocytes and macrophages. CD74 is also found on a subset of activated T cells, Langerhans cells, dendritic cells, and some epithelial cells. CD74 may play a role in antigen processing and presentation through intracellular transport and expression of class II molecules on the cell surface. |
| Immunogen | Cell line SU-DHL-4 nuclei |
| Conjugate | BIOT (Biotin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications |
Flow Cytometry – Quality tested 4,6,10,11 Immunohistochemistry-Frozen Sections – Reported in literature 1,2 Immunohistochemistry-Paraffin Sections – Reported in literature 1,3-5 Immunocytochemistry – Reported in literature 1,6 Immunoprecipitation – Reported in literature 1,6 Western Blot – Reported in literature 7 Blocking – Reported in literature 8,9 |
| RRID Number | AB_2797093 |
| Gene ID |
972 (Human) |
| Gene ID Symbol |
CD74 (Human) |
| Gene ID Aliases | DHLAG; HLADG; II; Ia-GAMMA; p33 |
| UniProt ID |
P04233 (Human |
| UniProt Name |
HG2A_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Epstein AL, Marder RJ, Winter JN, Fox RI. Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol. 1984;133:1028-36. (Immunogen, IHC-FS, IHC-PS, ICC, IP)
- 2. Pack M, Trumpfheller C, Thomas D, Park CG, Granelli-Piperno A, Münz C, et al. DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp. Immunology. 2007;123:438-46. (IHC-FS)
- 3. Sarker AB, Akagi T, Jeon HJ, Miyake K, Murakami I, Yoshino T, et al. Bauhinia purpurea--a new paraffin section marker for Reed-Sternberg cells of Hodgkin's disease. A comparison with Leu-M1 (CD15), LN2 (CD74), peanut agglutinin, and Ber-H2 (CD30). Am J Pathol. 1992;141:19-23. (IHC-PS)
- 4. Burton JD, Ely S, Reddy PK, Stein R, Gold DV, Cardillo TM, et al. CD74 is expressed by multiple myeloma and is a promising target for therapy. Clin Cancer Res. 2004;10:6606-11. (IHC-PS, FC)
- 5. Sepp N, Radaszkiewicz T, Meijer CJ, Smolle J, Seewann H, Fritsch P, et al. Specific skin manifestations in acute leukemia with monocytic differentiation. A morphologic and immunohistochemical study of 11 cases. Cancer. 1993;71:124-32. (IHC-PS)
- 6. Triantafilou K, Triantafilou M, Wilson KM, Cherry RJ, Fernandez N. Intracellular and cell surface heterotypic associations of human leukocyte antigen-DR and human invariant chain. Hum Immunol. 1999;60:1101-12. (ICC, IP, FC)
- 7. Imam A, Taylor CR, inventors; University of Southern California, assignee. Antigen specifically expressed on the surface of B cells and Hodgkin's cells. United States patent US 5304635. 1994 Apr 19. (WB)
- 8. Zhou C, Lu L, Tan S, Jiang S, Chen Y. HIV-1 glycoprotein 41 ectodomain induces activation of the CD74 protein-mediated extracellular signal-regulated kinase/mitogen-activated protein kinase pathway to enhance viral infection. J Biol Chem. 2011;286:44869-77. (Block)
- 9. Klasen C, Ohl K, Sternkopf M, Schachar I, Schmitz C, Heussen N, et al. MIF promotes B cell chemotaxis through the receptors CXCR4 and CD74 and ZAP-70 signaling. J Immunol. 2014;192:5273-84. (Block)
- 10. Aiello A, Delia D, Borrello MG, Biassoni D, Giardini R, Fontanella E, et al. Flow cytometric detection of the mitochondrial BCL-2 protein in normal and neoplastic human lymphoid cells. Cytometry. 1992;13:502-9. (FC)
- 11. Butrym A, Majewski M, Dzietczenia J, Kuliczkowski K, Mazur G. High CD74 expression correlates with ZAP70 expression in B cell chronic lymphocytic leukemia patients. Med Oncol. 2013;30:560. (FC)
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