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Clone | F10-89-4 |
---|---|
Isotype | Mouse IgG2aκ |
Isotype Control | Mouse IgG2a-FITC (HOPC-1) |
Specificity | Human/Horse CD45 |
Alternative Names | LCA, T200, Ly5 |
Description | CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220 kDa) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation. |
Immunogen | Purified T cells from human lymph nodes |
Conjugate | FITC (Fluorescein) |
Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
Clonality | Monoclonal |
Concentration | Lot specific |
Volume | 0.25 mL or 1.0 mL |
Recommended Storage | 2-8°C; Avoid exposure to light |
Applications |
Flow Cytometry – Quality tested 1,5,9-11 Immunohistochemistry-Frozen Sections – Reported in literature 1-3 Immunocytochemistry – Reported in literature 4,5 Immunoprecipitation – Reported in literature 6,7 Western Blot – Reported in literature 7,8 |
RRID Number | AB_2797023 |
Gene ID |
5788 (Human) 100061950 (Horse) |
Gene ID Symbol |
PTPRC (Human) PTPRC (Horse) |
Gene ID Aliases | B220; CD45; CD45R; GP180; L-CA; LCA; LY5; T200 |
UniProt ID |
P08575 (Human |
UniProt Name |
PTPRC_HUMAN (Human) |
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