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Mouse Anti-Human CD45-AF647 (2D1)
Cat. No.:
9624-31
Alexa Fluor® 647 Anti-Human CD45 antibody for use in flow cytometry, immunohistochemistry, and western blot assays.
$253.00
| Clone | 2D1 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-AF647 (15H6) |
| Specificity | Human CD45 |
| Alternative Names | LCA, T200, Ly5 |
| Description | CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220 kDa) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation. |
| Immunogen | Human peripheral blood mononuclear cells |
| Conjugate | AF647 (Alexa Fluor® 647) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications | Flow Cytometry – Quality tested 6 Immunohistochemistry-Frozen Sections – Reported in literature 1-3 Immunohistochemistry-Paraffin Sections – Reported in literature 4 IP-Non-Reducing – Reported in literature 5,6 Western Blot-Non-Reducing – Reported in literature 5 |
| RRID Number | AB_2797020 |
| Gene ID | 5788 (Human) |
| Gene ID Symbol | PTPRC (Human) |
| Gene ID Aliases | B220; CD45; CD45R; GP180; L-CA; LCA; LY5; T200 |
| UniProt ID | P08575 (Human |
| UniProt Name | PTPRC_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Gillitzer R, Berger R, Moll H. A reliable method for simultaneous demonstration of two antigens using a novel combination of immunogold-silver staining and immunoenzymatic labeling. J Histochem Cytochem. 1990;38:307-13. (IHC-FS)
- 2. Nickoloff BJ, Kunkel SL, Burdick M, Strieter RM. Severe combined immunodeficiency mouse and human psoriatic skin chimeras. Validation of a new animal model. Am J Pathol. 1995;146:580-8. (IHC-FS)
- 3. Ménétrier-Caux C, Bain C, Favrot MC, Duc A, Blay JY. Renal cell carcinoma induces interleukin 10 and prostaglandin E2 production by monocytes. Br J Cancer. 1999;79:119-30. (IHC-FS)
- 4. Charbord P, Tavian M, Humeau L, Péault B. Early ontogeny of the human marrow from long bones: an immunohistochemical study of hematopoiesis and its microenvironment. Blood. 1996;87:4109-19. (IHC-PS)
- 5. Poggi A, Pardi R, Pella N, Morelli L, Sivori S, Vitale M, et al. CD45-mediated regulation of LFA1 function in human natural killer cells. Anti-CD45 monoclonal antibodies inhibit the calcium mobilization induced via LFA1 molecules. Eur J Immunol. 1993;23:2454-63. (IP, WB)
- 6. Lazarovits AI, Osman N, Le Feuvre CE, Ley SC, Crumpton MJ. CD7 is associated with CD3 and CD45 on human T cells. J Immunol. 1994;153:3956-66. (IP, FC)
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