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Mouse Anti-Human CD35-PE (E11)
Cat. No.:
9600-09
PE Anti-Human CD35 antibody for use in flow cytometry assays.
$240.00
| Clone | E11 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-PE (15H6) |
| Specificity | Human/Rhesus/Cynomolgus/Chimpanzee/Baboon CD35 |
| Alternative Names | CR1, complement receptor type 1, C3bR, C4bR, immune adherence receptor |
| Description | The CD35 antigen is composed of four different allotypes having different molecular weights [A (or F) - 160 kDa, B (or S) - 190 kDa, C - 220 kDa, and D - 250 kDa]. It is the receptor for C3b and is found on B cells, a subset of T cells, monocytes, neutrophils, follicular dendritic cells, erythrocytes, and kidney glomelular podocytes. CD35 mediates neutrophil and monocyte phagocytosis of particles coated with C3b and/or C4b. This interaction also enhances phagocytosis mediated by the Fc receptors. |
| Immunogen | Acute monocytic leukemia cells and normal blood monocytes |
| Conjugate | PE (R-phycoerythrin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
| Applications |
Flow Cytometry – Quality tested 8,11 Immunohistochemistry-Frozen Sections – Reported in literature 7 Immunohistochemistry-Paraffin Sections – Reported in literature 6 Immunoprecipitation – Reported in literature 1,2 Western Blot – Reported in literature 3-5,9 ELISA – Reported in literature 1,2,4,5 |
| RRID Number | AB_2796997 |
| Gene ID |
1378 (Human) 718120 (Rhesus) 102117401 (Cynomolgus) 449643 (Chimpanzee) 101016492 (Baboon) |
| Gene ID Symbol |
CR1 (Human) CR1 (Rhesus) CR1 (Cynomolgus) CR1 (Chimpanzee) LOC101016492 (Baboon) |
| Gene ID Aliases | C3BR; C4BR; CD35; KN; CK820_G0012775 |
| UniProt ID |
P17927 (Human |
| UniProt Name |
CR1_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Krych M, Hourcade D, Atkinson JP. Sites within the complement C3b/C4b receptor important for the specificity of ligand binding. Proc Natl Acad Sci USA. 1991;88:4353-7. (IP, ELISA)
- 2. Moulds JM, Nickells MW, Moulds JJ, Brown MC, Atkinson JP. The C3b/C4b receptor is recognized by the Knops, McCoy, Swain-langley, and York blood group antisera. J Exp Med. 1991;173:1159-63. (IP, ELISA)
- 3. Dervillez X, Oudin S, Libyh MT, Tabary T, Reveil B, Philbert F, et al. Catabolism of the human erythrocyte C3b/C4b receptor (CR1, CD35): vesiculation and/or proteolysis?. Immunopharmacology. 1997;38:129-40. (WB)
- 4. Nickells M, Hauhart R, Krych M, Subramanian VB, Geoghegan-Barek K, Marsh HC Jr, et al. Mapping epitopes for 20 monoclonal antibodies to CR1. Clin Exp Immunol. 1998;112:27-33. (WB, ELISA)
- 5. Birmingham DJ, Gavit KF, McCarty SM, Yu CY, Rovin BH, Nagaraja HN, et al. Consumption of erythrocyte CR1 (CD35) is associated with protection against systemic lupus erythematosus renal flare. Clin Exp Immunol. 2005;143:274-80. (WB, ELISA)
- 6. Norton AJ, Isaacson PG. Detailed phenotypic analysis of B-cell lymphoma using a panel of antibodies reactive in routinely fixed wax-embedded tissue. Am J Pathol. 1987;128:225-40. (IHC-PS)
- 7. Daridon C, Loddenkemper C, Spieckermann S, Kühl AA, Salama A, Burmester GR, et al. Splenic proliferative lymphoid nodules distinct from germinal centers are sites of autoantigen stimulation in immune thrombocytopenia. Blood. 2012;120:5021-31. (IHC-FS)
- 8. Yoshino N, Ami Y, Terao K, Tashiro F, Honda M. Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim. 2000;49:97-110. (FC, Cynomolgus Reactivity)
- 9. Chen W, Logar CM, Shen X, Birmingham DJ. The chimpanzee and cynomolgus monkey erythrocyte immune adherence receptors are encoded by CR1-like genes. Immunogenetics. 2000;52:46-52. (WB, Cynomolgus & Chimpanzee Reactivity)
- 10. Birmingham DJ, Logar CM, Shen X, Chen W. The baboon erythrocyte complement receptor is a glycophosphatidylinositol-linked protein encoded by a homologue of the human CR1-like genetic element. J Immunol. 1996;157:2586-92. (WB, Baboon Reactivity)
- 11. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dörries R, ter Meulen V. Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys. Cytometry. 1997;29:351-62. (FC, Rhesus Reactivity)
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