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Mouse Anti-Human CD16-LE/AF (3G8)
Cat. No.:
9571-14
Low Endotoxin/Azide Free Anti-Human CD16 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, western blot, immunoprecipitation, stimulation, costimulation, and blocking assays.
$369.00
| Clone | 3G8 |
|---|---|
| Isotype | Mouse (CD2F1) IgG1κ |
| Isotype Control | Mouse IgG1-LE/AF (15H6) |
| Specificity | Human/Chimpanzee CD16 |
| Alternative Names | FcγRIII, low affinity Fc receptor |
| Description | CD16, a member of the immunoglobulin superfamily, is a 50-65 kDa glycoprotein found as both a transmembrane and GPI-linked form. The transmembrane form of CD16 is expressed on NK cells, granulocytes, macrophages, and mast cells but not on eosinophils. The GPI-anchored type of CD16 is found only on neutrophils. CD16 is involved in NK activation and signal transduction. |
| Immunogen | Human polymorphonuclear leukocytes |
| Conjugate | LE/AF (Low Endotoxin/Azide Free) |
| Buffer Formulation | Phosphate buffered saline, pH 7.4 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Handle under aseptic conditions |
| Applications |
Flow Cytometry – Quality tested 2,3,5-7 Immunohistochemistry-Frozen Sections – Reported in literature 4 Immunocytochemistry – Reported in literature 3 Immunoprecipitation – Reported in literature 1,2 Western Blot-Non-Reducing – Reported in literature 3 Stimulation – Reported in literature 5 Costimulation – Reported in literature 5 Blocking – Reported in literature 1 |
| RRID Number | AB_2796965 |
| Gene ID |
2214 (Human) 2215 (Human) 457457 (Chimpanzee) 745350 (Chimpanzee) |
| Gene ID Symbol |
FCGR3A (Human) FCGR3B (Human) LOC457457 (Chimpanzee) LOC745350 (Chimpanzee) |
| Gene ID Aliases | CD16; CD16A; FCG3; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20; CD16b; FCGR3A; FCRIIIb; CK820_G0056287; FCGR3B |
| UniProt ID |
P08637 (Human O75015 (Human |
| UniProt Name |
FCG3A_HUMAN (Human) FCG3B_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Fleit HB, Wright SD, Unkeless JC. Human neutrophil Fcγ receptor distribution and structure. Proc Natl Acad Sci USA. 1982;79:3275-9. (Immunogen, Block, IP)
- 2. Edberg JC, Kimberly RP. Cell type-specific glycoforms of FcγRIIIa (CD16) differential ligand binding. J Immunol. 1997;159:3849-57. (FC, IP)
- 3. Tosi MF, Zakem H. Surface expression of Fcγ receptor III (CD16) on chemoattractant-stimulated neutrophils is determined by both surface shedding and translocation from intracellular storage compartments. J Clin Invest. 1992;90:462-70. (FC, ICC, WB)
- 4. Sedmak DD, Davis DH, Singh U, van de Winkel JG, Anderson CL. Expression of IgG Fc receptor antigens in placenta and on endothelial cells in humans. An immunohistochemical study. Am J Pathol. 1991;138:175-81. (IHC-FS)
- 5. Hoshino S, Oshimi K, Teramura M, Mizoguchi H. Activation via the CD3 and CD16 pathway mediates interleukin-2-dependent autocrine proliferation of granular lymphocytes in patients with granular lymphocyte proliferative disorders. Blood. 1991;78:3232-40. (FC, Stim, Costim)
- 6. Mavoungou E, Bouyou-Akotet MK, Kremsner PG. Effects of prolactin and cortisol on natural killer (NK) cell surface expression and function of human natural cytotoxicity receptors (NKp46, NKp44 and NKp30). Clin Exp Immunol. 2004;139:287-96. (FC)
- 7. Reeves RK, Evans TI, Fultz PN, Johnson RP. Potential confusion of contaminating CD16+ myeloid DCs with anergic CD16+ NK cells in chimpanzees. Eur J Immunol. 2011;41:1070-4. (FC, Chimpanzee Reactivity)
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