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Mouse Anti-Human CD14-AF647 (61D3)
Cat. No.:
9561-31
Alexa Fluor® 647 Anti-Human CD14 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blot assays.
$253.00
| Clone | 61D3 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-AF647 (15H6) |
| Specificity | Human/Cynomolgus/Canine/Hooded Seal CD14 |
| Alternative Names | LPS-R, LPS receptor, gp55 |
| Description | CD14 is a 55 kDa glycosphosphatidylinositol (GPI)-anchored single chain glycoprotein. It is expressed on monocytes, macrophages, dendritic cells, and Langerhans cells. It is also weakly expressed on neutrophils. CD14 appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes. |
| Immunogen | Human peripheral monocytes |
| Conjugate | AF647 (Alexa Fluor® 647) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications |
Flow Cytometry – Quality tested 3,6-8 Immunohistochemistry-Frozen Sections – Reported in literature 4 Immunocytochemistry – Reported in literature 3,5 Western Blot – Reported in literature 3 ELISA – Reported in literature 3 Cytoxicity – Reported in literature 1 Blocking – Reported in literature 2,3 Separation – Reported in literature 9 |
| RRID Number | AB_2796947 |
| Gene ID |
929 (Human) 102129342 (Cynomolgus) 607076 (Canine) |
| Gene ID Symbol |
CD14 (Human) CD14 (Cynomolgus) CD14 (Canine) |
| UniProt ID |
P08571 (Human |
| UniProt Name |
CD14_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Ugolini V, Nunez G, Smith RG, Stastny P, Capra JD. Initial characterization of monoclonal antibodies against human monocytes. Proc Natl Acad Sci USA. 1980;77:6764-8. (Immunogen, Cyto)
- 2. Flora PK, Gregory CD. Recognition of apoptotic cells by human macrophages: inhibition by a monocyte/macrophage-specific monoclonal antibody. Eur J Immunol. 1994;24:2625-32. (Block)
- 3. Devitt A, Moffatt OD, Raykundalia C, Capra JD, Simmons DL, Gregory CD. Human CD14 mediates recognition and phagocytosis of apoptotic cells. Nature. 1998;392:505-9. (Block, WB, FC, ELISA, ICC)
- 4. Beckstead JH, Wood GS, Fletcher V. Evidence for the origin of Kaposi's sarcoma from lymphatic endothelium. Am J Pathol. 1985;119:294-300. (IHC-FS)
- 5. Larsen AK, Nymo IH, Boysen P, Tryland M, Godfroid J. Entry and elimination of marine mammal Brucella spp. by hooded seal (Cystophora cristata) alveolar macrophages in vitro. PLoS One. 2013;8(7):e70186. (ICC, Hooded Seal Reactivity)
- 6. Pilling D, Vakil V, Gomer RH. Improved serum-free culture conditions for the differentiation of human and murine fibrocytes. J Immunol Methods. 2009;351:62-70. (FC)
- 7. Carter AS, Cerundolo L, Koo DD, Rust NA, Morris PJ, Fuggle SV. Cross-species reactivity of a panel of antibodies with monkey and porcine tissue. Xenotransplantation. 1999;6:123-30. (FC, Cynomolgus Reactivity)
- 8. de Amorim IF, da Silva SM, Figueiredo MM, Moura EP, de Castro RS, de Souza Lima TK, et al. Toll receptors type-2 and CR3 expression of canine monocytes and its correlation with immunohistochemistry and xenodiagnosis in visceral leishmaniasis. PLoS One. 2011;6(11):e27679. (FC, Canine Reactivity)
- 9. Lindsey JW, Hatfield LM. Epstein-Barr virus and multiple sclerosis: cellular immune response and cross-reactivity. J Neuroimmunol. 2010;229:238-42. (Sep)
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