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Mouse Anti-Human CD107b-AF488 (H4B4)
Cat. No.:
9840-30
Alexa Fluor® 488 Anti-Human CD107b antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blot assays.
$259.00
| Clone | H4B4 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-AF488 (15H6) |
| Specificity | Human/Rhesus/African Green Monkey CD107b |
| Alternative Names | LAMP-2, lysosome-associated membrane protein 2 |
| Description | CD107b, also known as lysosomal-associated membrane protein 2 (LAMP-2), is a heavily glycosylated type I transmembrane protein that constitutes the major sialoglycoproteins on lysosomal membranes. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains and is a ligand for E-selectin-mediated cell adhesion. CD107b is expressed in lysosomal/endosomal membranes and on the surface of activated platelets and some tumor cell lines including U937 and KG1a. LAMP-2 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. The upregulation of both CD107a and CD107b on the surface of tumor cell lines has been associated with their enhanced metastatic potential where they may increase adhesion to extracellular matrix and endothelium. |
| Immunogen | Human adherent peripheral blood cells |
| Conjugate | AF488 (Alexa Fluor® 488) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications |
Flow Cytometry – Quality tested 1,15,16 Immunohistochemistry-Frozen Sections – Reported in literature 2,3 Immunohistochemistry-Paraffin Sections – Reported in literature 4,5 Immunocytochemistry – Reported in literature 6-9,11,17-19 Electron Microscopy – Reported in literature 1 Immunoprecipitation – Reported in literature 1 Western Blot – Reported in literature 9-14 Purification – Reported in literature 1 |
| RRID Number | AB_2797120 |
| Gene ID |
3920 (Human) 695379 (Rhesus) 103247358 (Green Monkey) |
| Gene ID Symbol |
LAMP2 (Human) LAMP2 (Rhesus) LAMP2 (Green Monkey) |
| Gene ID Aliases | CD107b; LAMP-2; LAMPB; LGP-96; LGP110; EGK_20887 |
| UniProt ID |
P13473 (Human |
| UniProt Name |
LAMP2_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view
- 1. Mane SM, Marzella L, Bainton DF, Holt VK, Cha Y, Hildreth JE, et al. Purification and characterization of human lysosomal membrane glycoproteins. Arch Biochem Biophys. 1989;268:360-78. (Immunogen, FC, EM, IP, Purification)
- 2. Nishino I, Fu J, Tanji K, Yamada T, Shimojo S, Koori T, et al. Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease). Nature. 2000;406:906-10. (IHC-FS)
- 3. Yan C, Tanaka M, Sugie K, Nobutoki T, Woo M, Murase N, et al. A new congenital form of X-linked autophagic vacuolar myopathy. Neurology. 2005;65:1132-4. (IHC-FS)
- 4. Furuta K, Ikeda M, Nakayama Y, Nakamura K, Tanaka M, Hamasaki N, et al. Expression of lysosome-associated membrane proteins in human colorectal neoplasms and inflammatory diseases. Am J Pathol. 2001;159:449-55. (IHC-PS)
- 5. Sarafian VS, Dikov DI. LAMPs and ABH histo-blood group antigens in granulation tissue. APMIS. 2007;115:701-6. (IHC-PS)
- 6. Schnaith A, Kashkar H, Leggio SA, Addicks K, Krönke M, Krut O. Staphylococcus aureus subvert autophagy for induction of caspase-independent host cell death. J Biol Chem. 2007;282:2695-706. (ICC)
- 7. Robinson N, Kolter T, Wolke M, Rybniker J, Hartmann P, Plum G. Mycobacterial phenolic glycolipid inhibits phagosome maturation and subverts the pro-inflammatory cytokine response. Traffic. 2008;9:1936-47. (ICC)
- 8. Groth-Pedersen L, Aits S, Corcelle-Termeau E, Petersen NH, Nylandsted J, Jäättelä M. Identification of cytoskeleton-associated proteins essential for lysosomal stability and survival of human cancer cells. PLoS One. 2012;7:e45381. (ICC)
- 9. Romao S, Gasser N, Becker AC, Guhl B, Bajagic M, Vanoaica D, et al. Autophagy proteins stabilize pathogen-containing phagosomes for prolonged MHC II antigen processing. J Cell Biol. 2013;203:757-66. (ICC)
- 10. Appelqvist H, Nilsson C, Garner B, Brown AJ, Kågedal K, Öllinger K. Attenuation of the lysosomal death pathway by lysosomal cholesterol accumulation. Am J Pathol. 2011;178:629-39. (ICC, WB)
- 11. Appelqvist H, Sandin L, Björnström K, Saftig P, Garner B, Öllinger K, et al. Sensitivity to lysosome-dependent cell death is directly regulated by lysosomal cholesterol content. PLoS One. 2012;7:e50262. (WB, ICC)
- 12. Domert J, Rao SB, Agholme L, Brorsson A, Marcusson J, Hallbeck M, et al. Spreading of amyloid-β peptides via neuritic cell-to-cell transfer is dependent on insufficient cellular clearance. Neurobiol Dis. 2014;65:82-92. (WB)
- 13. Armstrong A, Mattsson N, Appelqvist H, Janefjord C, Sandin L, Agholme L, et al. Lysosomal network proteins as potential novel CSF biomarkers for Alzheimer's disease. Neuromolecular Med. 2014;16:150-60. (WB)
- 14. Fritsch J, Stephan M, Tchikov V, Winoto-Morbach S, Gubkina S, Kabelitz D, et al. Cell fate decisions regulated by k63 ubiquitination of tumor necrosis factor receptor 1. Mol Cell Biol. 2014;34:3214-27. (WB)
- 15. Genescà M, Skinner PJ, Bost KM, Lu D, Wang Y, Rourke TL, et al. Protective attenuated lentivirus immunization induces SIV-specific T cells in the genital tract of rhesus monkeys. Mucosal Immunol. 2008;1:219-28. (FC, Rhesus Reactivity)
- 16. Cicin-Sain L, Sylwester AW, Hagen SI, Siess DC, Currier N, Legasse AW, et al. Cytomegalovirus-specific T cell immunity is maintained in immunosenescent rhesus macaques. J Immunol. 2011;187:1722-32. (FC, Rhesus Reactivity)
- 17. Tabuchi M, Yoshimori T, Yamaguchi K, Yoshida T, Kishi F. Human NRAMP2/DMT1, which mediates iron transport across endosomal membranes, is localized to late endosomes and lysosomes in HEp-2 cells. J Biol Chem. 2000;275:22220-8. (ICC, African Green Monkey Reactivity)
- 18. Knox C, Moffat K, Ali S, Ryan M, Wileman T. Foot-and-mouth disease virus replication sites form next to the nucleus and close to the Golgi apparatus, but exclude marker proteins associated with host membrane compartments. J Gen Virol. 2005;86:687-96.(ICC, African Green Monkey Reactivity)
- 19. Tam J, Cordier GA, Borbely JS, Álvarez ÁS, Lakadamyali M. Cross-talk-free multi-color STORM imaging using a single fluorophore. PLoS One. 2014;9(7):e101772. (ICC, African Green Monkey Reactivity)
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