Mouse Anti-His-Tag-HRP (SB194b)
Cat. No.:
4603-05
HRP Anti-His-Tag for use in ELISA and western blotting assays.
$380.00
![6X His-tag was immunoprecipitated from CHO-K1 cell lysates transfected with GFP - 6X His-tag with Mouse Anti-His-Tag-UNLB (SB Cat. No. 4603-01). Lysates were resolved by electrophoresis, transferred to PVDF membrane, probed with Mouse Anti-His-Tag-HRP (SB Cat. No. 4603-05), and visualized using chemiluminescent detection.](https://www.southernbiotech.com/media/catalog/product/cache/7149a3d30fe6da616557c86e375dcb18/b/e/bebb29736bedd1ea9b69fa13626b6c04a7943a98605fe764c7ed0b0788cde1e9.jpeg)
![6X His-tag was immunoprecipitated from CHO-K1 cell lysates transfected with GFP - 6X His-tag with Mouse Anti-His-Tag-UNLB (SB Cat. No. 4603-01). Lysates were resolved by electrophoresis, transferred to PVDF membrane, probed with Mouse Anti-His-Tag-HRP (SB Cat. No. 4603-05), and visualized using chemiluminescent detection.](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/b/e/bebb29736bedd1ea9b69fa13626b6c04a7943a98605fe764c7ed0b0788cde1e9.jpeg)
![CHO-K1 cell lysates transfected with GFP - 6X His-tag were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-HRP (SB Cat. No. 4603-05) followed by chemiluminescent detection.](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/3/7/379c0ca94369b9914ae6c614775bb5ff507e2f91dad3378b12ff3cda89d75eb3.jpeg)
![ELISA plate was coated with N-terminal 6X His-tag conjugated to BSA and BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-HRP (SB Cat. No. 4603-05).](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/3/8/38ee4e4d2f239022178185016f2de8a54a21f3febb0ff1ee8ca4b6813055e1ad.jpeg)
Clone | SB194b |
---|---|
Isotype | Mouse (BALB/c) IgG1κ |
Isotype Control | Mouse IgG1-HRP (15H6) |
Specificity |
His-tag Reacts with N-terminal, C-terminal, and internal repeating histidine residues of 6 or more |
Immunogen | Synthetic 6X His-tag peptides |
Conjugate | HRP (Horseradish Peroxidase) |
Buffer Formulation | 50% Glycerol/50% Phosphate buffered saline, pH 7.4 |
Clonality | Monoclonal |
Concentration | Lot specific |
Volume | 1.0 mL |
Recommended Storage | 2-8°C |
Applications |
ELISA – Quality tested 1 FLISA – Quality tested 1 Immunoprecipitation – Quality tested 1 Western blot 1 Purification 1 Flow Cytometry 1 Immunocytochemistry 1 |
RRID Number | AB_2819136 |
Documentation
Certificate of Analysis Lookup
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