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  • Mouse Anti-Goat IgG Fc-HRP (SB115d)

Mouse Anti-Goat IgG Fc-HRP (SB115d)

Cat. No.:
6158-05
HRP Anti-Goat IgG Fc antibody for use in ELISA, immunohistochemistry, and western blot assays.
$330.00
Size Price (USD) Quantity
1.0 mL $330.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone SB115d
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-HRP (HOPC-1)
Specificity Goat IgG Fc
Minimal reactivity to goat IgG Fab and sheep, rabbit, human, monkey, rat, hamster, canine, and swine IgG
Immunogen Pooled goat IgG
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested 1
FLISA – Quality tested
Western Blot – Reported in literature 2
Immunohistochemistry-Frozen Sections 3

RRID Number AB_2796222

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Tang J, Sun X, Yang H, Zhang B, Li Z, Lin Z, et al. Well-oriented ZZ-PS-tag with high Fc-binding onto polystyrene surface for controlled immobilization of capture antibodies. Anal Chim Acta. 2013;776:74-8. (ELISA)
  2. 2. Mohapatra A, Van Dyken SJ, Schneider C, Nussbaum JC, Liang H, Locksley RM. Group 2 innate lymphoid cells utilize the IRF4-IL-9 module to coordinate epithelial cell maintenance of lung homeostasis. Mucosal Immunol. 2016;9:275-86. (WB)
  3. 3. SouthernBiotech published data (IHC-FS)

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  3. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit F(ab')<sub>2</sub> Anti-Goat IgG(H+L), Human SP ads-HRP (SB Cat. No. 6026-05).
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  5. ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6157-05).
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  6. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit Anti-Goat IgG(H+L)-HRP (SB Cat. No. 6160-05).
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  7. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit Anti-Goat IgG(H+L), Human SP ads-HRP (SB Cat. No. 6164-05).
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  8. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit F(ab')<sub>2</sub> Anti-Goat IgG(H+L)-HRP (SB Cat. No. 6020-05).
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  9. ELISA plate was coated with serially diluted Goat IgG Fc-UNLB (SB Cat. No. 0130-01). Immunoglobulin was detected with Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) and Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  10. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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  12. Paraffin embedded mouse spleen section was stained with Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-HRP (SB Cat. No. 6420-05), DAB, and hematoxylin.
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  13. ELISA plate was coated with serially diluted Goat F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0110-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) and Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  14. Purified Human Type I Collagen (SB Cat. No. 1200-01S) was resolved by electrophoresis, transferred to PVDF membrane, probed with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01), and visualized using Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6425-05) secondary antibody and chemiluminescent detection.
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  15. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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