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  • Mouse Anti-Goat IgG Fc-BIOT (SB115d)

Mouse Anti-Goat IgG Fc-BIOT (SB115d)

Cat. No.:
6158-08
Biotin Anti-Goat IgG Fc antibody for use in ELISA, immunohistochemistry, and western blot assays.
$286.00
Size Price (USD) Quantity
0.5 mg $286.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone SB115d
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-BIOT (HOPC-1)
Specificity Goat IgG Fc
Minimal reactivity to goat IgG Fab and sheep, rabbit, human, monkey, rat, hamster, canine, and swine IgG
Immunogen Pooled goat IgG
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested 1
FLISA – Quality tested
Western Blot – Reported in literature 2
Immunohistochemistry-Frozen Sections 3

RRID Number AB_2796223

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Tang J, Sun X, Yang H, Zhang B, Li Z, Lin Z, et al. Well-oriented ZZ-PS-tag with high Fc-binding onto polystyrene surface for controlled immobilization of capture antibodies. Anal Chim Acta. 2013;776:74-8. (ELISA)
  2. 2. Mohapatra A, Van Dyken SJ, Schneider C, Nussbaum JC, Liang H, Locksley RM. Group 2 innate lymphoid cells utilize the IRF4-IL-9 module to coordinate epithelial cell maintenance of lung homeostasis. Mucosal Immunol. 2016;9:275-86. (WB)
  3. 3. SouthernBiotech published data (IHC-FS)

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  2. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
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  3. ELISA plate was coated with purified goat IgG Fab and IgG Fc. Immunoglobulins were detected with serially diluted Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  4. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45R-BIOT (SB Cat. No. 1665-08) followed by Mouse Anti-Biotin-PE (SB Cat. No. 6404-09).
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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  7. Paraffin embedded rat kidney section post 30% blood volume loss and treatment with ketoprofen was stained with anti-IL-1 followed by Rabbit Anti-Goat IgG(H+L)-BIOT (SB Cat. No. 6160-08), HRP conjugated streptavidin, DAB, and hematoxylin.<br>Image from Guedes FS Jr, Cruz DS, Rodrigues MM, Silva LM, Amorim RL, Vianna PT, et al. Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect. Acta Cir Bras. 2012;27:37-42. Figure 3
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  10. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT (SB Cat. No. 6300-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
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  12. Frozen newborn mouse cartilage section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01; right) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-BIOT (SB Cat. No. 6420-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  13. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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