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  • Mouse Anti-Cytokeratin 18-HRP (SB38b)

Mouse Anti-Cytokeratin 18-HRP (SB38b)

Cat. No.:
10085-05
HRP Anti-Cytokeratin 18 antibody for use in immunohistochemistry / immunocytochemistry, ELISA, and western blot assays.
$330.00
Size Price (USD) Quantity
1.0 mL $330.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone SB38b
Isotype Mouse (BALB/c) IgG2bκ
Isotype Control Mouse IgG2b-HRP (A-1)
Specificity Cytokeratin 18
Alternative Names CK 18, keratin 18
Description Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology. Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 18 is a type I intermediate filament protein that exists as a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 is expressed in all simple type epithelia and basal cells of many squamous and non-epidermal epithelia and is often co-expressed with cytokeratin 8. Tissues from the gastrointestinal tract, respiratory tract, and urogenital tract as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Immunogen Recombinant N-terminal cytokeratin 18
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested
FLISA – Quality tested
Immunohistochemistry-Paraffin Sections 1
Immunocytochemistry 1
Flow Cytometry 2
Western Blot 2

RRID Number AB_2794115
Gene ID 3875 (Human)
Gene ID Symbol KRT18 (Human)
Gene ID Aliases CK-18; CYK18; K18
UniProt ID P05783 (Human
UniProt Name K1C18_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. SouthernBiotech published data (IHC-PS, ICC)
  2. 2. SouthernBiotech unpublished data (FC, WB)

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  1. Paraffin embedded human gastric cancer tissue was stained with Mouse IgG<sub>2b</sub>-HRP isotype control (SB Cat. No. 0104-05; left) and Mouse Anti-Cytokeratin 18-HRP (SB Cat. No. 10085-05; right) followed by DAB and hematoxylin.
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  2. Paraffin embedded human gastric cancer tissue was stained with Mouse IgG<sub>2a</sub>-HRP isotype control (SB Cat. No. 0103-05; left) and Mouse Anti-Cytokeratin 8-HRP (SB Cat. No. 10080-05; right) followed by DAB and hematoxylin.
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  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  4. Frozen MC4R-KO mouse liver section was stained with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01) and anti-F4/80 followed by secondary antibodies and DAPI.<br/>Image from Itoh M, Kato H, Suganami T, Konuma K, Marumoto Y, Terai S, et al. Hepatic crown-like structure: a unique histological feature in non-alcoholic steatohepatitis in mice and humans. PLoS One. 2013;8(12):e82163. Figure 2(e)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/3.0/
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  5. Paraffin embedded human gastric cancer tissue was stained with Mouse IgG<sub>2a</sub>-HRP isotype control (SB Cat. No. 0103-05; left) and Mouse Anti-Cytokeratin 19-HRP (SB Cat. No. 10090-05; right) followed by DAB and hematoxylin.
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  6. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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