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Mouse Anti-Chicken TCRγδ-AF488 (TCR-1)
Cat. No.:
8230-30
Alexa Fluor® 488 Anti-Chicken TCRγ/δ antibody for use in flow cytometry and immunohistochemistry assays.
$271.00
| Clone | TCR-1 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-AF488 (15H6) |
| Specificity | Chicken/Milk Snake TCRγδ |
| Alternative Names | T3/TCR complex, TCR gamma/delta |
| Description | The monoclonal antibody TCR-1 precipitates a heterodimer of Mr 90 kDa (two bands of Mr 50 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 35 kDa and 32 kDa. Unlike human and mouse in which γδ cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of γδ T cells. The frequency of TCRγδ cells is typically 20-25% of the total blood T cells but may reach approximately 50% in chickens of six- months of age. The majority of TCRγδ+ cells in the thymus and blood are CD4-CD8- although a small subset of them may express CD8 or CD4 coreceptors. However, when the TCRγδ+ cells migrate into the spleen and intestine most of them begin to express CD8. Although the biological function of γδ T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ γδ T cells may also be involved in down-regulation of the immune response. They cannot, however, induce a graft-vs-host (GVH) reactions. |
| Immunogen | Outbred chicken thymocytes and Ig-negative blood lymphocytes |
| Conjugate | AF488 (Alexa Fluor® 488) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 0.2 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications |
Flow Cytometry – Quality tested 1,2,8-13 Immunohistochemistry-Frozen Sections – Reported in literature 3,4 Immunohistochemistry-Paraffin Sections – Reported in literature 5,6 Immunoprecipitation – Reported in literature 1,2 Depletion – Reported in literature 7 |
| RRID Number | AB_2796453 |
Documentation
Certificate of Analysis Lookup
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- 1. Sowder JT, Chen C, Ager LL, Chan MM, Cooper MD. A large subpopulation of avian T cells express a homologue of the mammalian Tγ/δ receptor. J Exp Med. 1988;167:315-22. (Immunogen, FC, IP)
- 2. Chen CH, Cihak J, Lösch U, Cooper MD. Differential expression of two T cell receptors, TcR1 and TcR2, on chicken lymphocytes. Eur J Immunol. 1988;18:539-43. (FC, IP)
- 3. Tanimura N, Sharma JM. Appearance of T cells in the bursa of Fabricius and cecal tonsils during the acute phase of infectious bursal disease virus infection in chickens. Avian Dis. 1997;41:638-45. (IHC-FS)
- 4. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (IHC-FS, FC)
- 5. Solcan C, Solcan G, Cotea C. Immunotoxic action of ochratoxine A on lymphocytes from lymphoid tissues associated to gut mucosa in chickens. Bulletin UASVM Agriculture. 2010;67:283-90. (IHC-PS)
- 6. Teixeira AR, Gomes C, Nitz N, Sousa AO, Alves RM, Guimaro MC, et al. Trypanosoma cruzi in the chicken model: Chagas-like heart disease in the absence of parasitism. PLoS Negl Trop Dis. 2011;5(3):e1000. (IHC-PS)
- 7. Cihak J, Lösch U, Hoffmann-Fezer G, Chen CH, Cooper MD, Ziegler-Heitbrock HW. In vivo depletion of chicken T-cell subsets. Scand J Immunol. 1993;38:123-9. (Depletion)
- 8. Koskinen R, Göbel TW, Tregaskes CA, Young JR, Vainio O. The structure of avian CD5 implies a conserved function. J Immunol. 1998;160:4943-50. (FC)
- 9. Conrad ML, Davis WC, Koop BF. TCR and CD3 antibody cross-reactivity in 44 species. Cytometry. 2007;71A:925-33. (FC, Milk Snake Reactivity)
- 10. Schusser B, Collarini EJ, Yi H, Izquierdo SM, Fesler J, Pedersen D, et al. Immunoglobulin knockout chickens via efficient homologous recombination in primordial germ cells. Proc Natl Acad Sci USA. 2013;110:20170-5. (FC)
- 11. Laniewski P, Kuczkowski M, Chrząstek K, Woźniak A, Wyszyńska A, Wieliczko A, et al. Evaluation of the immunogenicity of Campylobacter jejuni CjaA protein delivered by Salmonella enterica sv. Typhimurium strain with regulated delayed attenuation in chickens. World J Microbiol Biotechnol. 2014;30:281-92. (FC)
- 12. Kjærup RM, Dalgaard TS, Norup LR, Hamzic E, Sørensen P, Juul-Madsen HR. Characterization of cellular and humoral immune responses after IBV infection in chicken lines differing in MBL serum concentration. Viral Immunol. 2014;27:529-42. (FC)
- 13. Pleidrup J, Dalgaard TS, Norup LR, Permin A, Schou TW, Skovgaard K, et al. Ascaridia galli infection influences the development of both humoral and cell-mediated immunity after Newcastle Disease vaccination in chickens. Vaccine. 2014;32:383-92. (FC)
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