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Mouse Anti-Chicken CD8β-FITC (EP42)
Cat. No.:
8280-02
FITC Anti-Chicken CD8β antibody for use in flow cytometry and immunohistochemistry assays.
$374.00
| Clone | EP42 |
|---|---|
| Isotype | Mouse (BALB/c) IgG2aκ |
| Isotype Control | Mouse IgG2a-FITC (HOPC-1) |
| Specificity | Chicken CD8β |
| Description | In the chicken, the CD8 molecule is present in two forms - (i) a homodimer of two α chains and (ii) a heterodimer of an α chain and a β chain. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8α- and CD8β-chains, a relatively large proportion of the CD8+ TCR-γδ cells in the spleens of embryos and young chicks express only the α-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCR-γδ CD8αβ cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. The monoclonal antibody EP42 recognizes the CD8β chain. |
| Immunogen | Chicken splenocytes |
| Conjugate | FITC (Fluorescein) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Applications |
Flow Cytometry – Quality tested 1,2,4-9 Immunohistochemistry-Frozen Sections – Reported in literature 2,3 Immunoprecipitation – Reported in literature 1 |
| RRID Number | AB_2796479 |
| Gene ID |
396175 (Chicken) |
| Gene ID Symbol |
CD8B (Chicken) |
| Gene ID Aliases | CD8B1; CD8BP |
Documentation
Certificate of Analysis Lookup
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- 1. Tregaskes CA, Kong F, Paramithiotis E, Chen CH, Ratcliffe MJ, Davison TF, et al. Identification and analysis of the expression of CD8αβ and CD8αα isoforms in chickens reveals a major TCR-γδ CD8αβ subset of intestinal intraepithelial lymphocytes. J Immunol. 1995;154:4485-94. (Immunogen, FC, IP)
- 2. van Haarlam DA, van Kooten PJ, Rothwell L, Kaiser P, Vervelde L. Characterisation and expression analysis of the chicken interleukin-7 receptor alpha chain. Dev Comp Immunol. 2009;33:1018-26. (FC, IHC-FS)
- 3. Pielsticker C. Investigations on the humoral and cell-mediated immune response in chicken after inoculation with Campylobacter jejuni strains of human and avian origin [thesis]. Hannover (Germany): University of Veterinary Medicine Hannover; 2013. (IHC-FS)
- 4. Buitenhuis AJ, Kjaer JB, Labouriau R, Juul-Madsen HR. Altered circulating levels of serotonin and immunological changes in laying hens divergently selected for feather pecking behavior. Poult Sci. 2006;85:1722-8. (FC)
- 5. van Ginkel FW, van Santen VL, Gulley SL, Toro H. Infectious bronchitis virus in the chicken Harderian gland and lachrymal fluid: viral load, infectivity, immune cell responses, and effects of viral immunodeficiency. Avian Dis. 2008;52:608-17. (FC)
- 6. Dalgaard T, Boving MK, Handberg K, Jensen KH, Norup LR, Juul-Madsen HR. MHC expression on spleen lymphocyte subsets in genetically resistant and susceptible chickens infected with Marek's disease virus. Viral Immunol. 2009;22:321-7. (FC)
- 7. Norup LR, Dalgaard TS, Friggens NC, Sørensen P, Juul-Madsen HR. Influence of chicken serum mannose-binding lectin levels on the immune response towards Escherichia coli. Poult Sci. 2009;88:543-53. (FC)
- 8. Petrov P, Motobu M, Salmi J, Uchida T, Vainio O. Novel leukocyte protein, Trojan, differentially expressed during thymocyte development. Mol Immunol. 2010;47:1522-8. (FC)
- 9. Mwangi WN, Smith LP, Baigent SJ, Beal RK, Nair V, Smith AL. Clonal structure of rapid-onset MDV-driven CD4+ lymphomas and responding CD8+ T cells. PLoS Pathog. 2011;7(5):e1001337. (FC)
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