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Mouse Anti-Chicken Bu-1b-PE (5-11G2)
Cat. No.:
8370-09
PE Anti-Chicken Bu-1b antibody for use in flow cytometry assays.
$237.00
| Clone | 5-11G2 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-PE (15H6) |
| Specificity | Chicken Bu-1b |
| Alternative Names | ChB6.2 |
| Description | Chicken Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 5-11G2 reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa |
| Immunogen | Bursa cells from two- to five-week-old CHA (Bu-1b) strain chickens |
| Conjugate | PE (R-phycoerythrin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
| Clonality | Monoclonal |
| Concentration | 0.1 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
| Applications | Flow Cytometry – Quality tested 1,10-16 Immunohistochemistry-Frozen Sections – Reported in literature 2-6 Immunocytochemistry – Reported in literature 1,7 Immunoprecipitation – Reported in literature 7,8 Apoptosis – Reported in literature 8,9 |
| RRID Number | AB_2796528 |
| Gene ID | 396098 (Chicken) |
| Gene ID Symbol | LOC396098 (Chicken) |
| Gene ID Aliases | B6.2; Bu-1; Bu-1b |
Documentation
Certificate of Analysis Lookup
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- 1. Veromaa T, Vainio O, Eerola E, Toivanen P. Monoclonal antibodies against chicken Bu-1a and Bu-1b alloantigens. Hybridoma. 1988;7:41-8. (Immunogen, ICC, FC)
- 2. Nagy N, Bíró É, Takács Á, Pólos M, Magyar A, Oláh I. Peripheral blood fibrocytes contribute to the formation of the avian spleen. Dev Dyn. 2005;232:55-66. (IHC-FS)
- 3. Felföldi B, Imre G, Igyártó B, Iván J, Mihalik R, Lackó E, et al. In ovo vitelline duct ligation results in transient changes of bursal microenvironments. Immunology. 2005;116:267-75. (IHC-FS)
- 4. Kabell S, Igyártó B, Magyar A, Hajdú Z, Bíró É, Bisgaard M, et al. Impact of heterophil granulocyte depletion caused by 5-fluorouracil on infectious bursal disease virus infection in specific pathogen free chickens. Avian Pathol. 2006;35:341-8. (IHC-FS)
- 5. Igyártó B, Magyar A, Oláh I. Origin of follicular dendritic cell in the chicken spleen. Cell Tissue Res. 2007;327:83-92. (IHC-FS)
- 6. Igyártó BZ, Nagy N, Magyar A, Oláh I. Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody. Poult Sci. 2008;87:351-5. (IHC-FS)
- 7. Li X, Rees JS, Xue P, Zhang H, Hamaia SW, Sanderson B, et al. New insights into the DT40 B cell receptor cluster using a proteomic proximity labeling assay. J Biol Chem. 2014;289:14434-47. (IP, ICC)
- 8. Funk PE, Pifer J, Kharas M, Crisafi G, Jonhnson A. The avian chB6 alloantigen induces apoptosis in DT40 B cells. Cell Immunol. 2003;226:95-104. (Apop, IP)
- 9. Narvi E, Nera K, Terho P, Mustonen L, Granberg J, Lassila O. Aiolos controls gene conversion and cell death in DT40 B cells. Scand J Immunol. 2007;65:503-13. (Apop)
- 10. Huffnagle GB, Ratcliffe MJ, Humphries EH. Bu-2, a novel avian cell surface antigen on B cells and a population of non-lymphoid cells, is expressed homogeneously in germinal centers. Hybridoma. 1989;8:589-604. (FC)
- 11. Ratcliffe MJ. Generation of immunoglobulin heavy chain diversity subsequent to cell surface immunoglobulin expression in the avian bursa of Fabricius. J Exp Med. 1989;170:1165-73. (FC)
- 12. Pararnithiotis E, Jacobsen KA, Ratcliffe MJ. Loss of surface immunoglobulin expression precedes B cell death by apoptosis in the bursa of Fabricius. J Exp Med. 1995;181:105-13. (FC)
- 13. Sayegh CE, Demaries SL, Iacampo S, Ratcliffe MJ. Development of B cells expressing surface immunoglobulin molecules that lack V(D)J-encoded determinants in the avian embryo bursa of fabricius. Proc Natl Acad Sci USA. 1999;96:10806-11. (FC)
- 14. Schulte CE, von Lindern M, Steinlein P, Beug H, Wiedemann LM. MLL-ENL cooperates with SCF to transform primary avian multipotent cells. EMBO J. 2002;21:4297-306. (FC)
- 15. Hunt HD, Goto RM, Foster DN, Bacon LD, Miller MM. At least one YMHCI molecule in the chicken is alloimmunogenic and dynamically expressed on spleen cells during development. Immunogenetics. 2006;58:297-307. (FC)
- 16. Petkov DI, Linnemann EG, Kapczynski DR, Sellers HS. Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens. Avian Dis. 2009;53:347-55. (FC)
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