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  • Mouse Anti-Chicken Bu-1b-FITC (5-11G2)

Mouse Anti-Chicken Bu-1b-FITC (5-11G2)

Cat. No.:
8370-02
FITC Anti-Chicken Bu-1b antibody for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.
$374.00
Size Price (USD) Quantity
0.5 mg $374.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone 5-11G2
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-FITC (15H6)
Specificity Chicken Bu-1b
Alternative Names ChB6.2
Description Chicken Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 5-11G2 reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa
Immunogen Bursa cells from two- to five-week-old CHA (Bu-1b) strain chickens
Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Flow Cytometry – Quality tested 1,10-16
Immunohistochemistry-Frozen Sections – Reported in literature 2-6
Immunocytochemistry – Reported in literature 1,7
Immunoprecipitation – Reported in literature 7,8
Apoptosis – Reported in literature 8,9

RRID Number AB_2796526
Gene ID 396098 (Chicken)
Gene ID Symbol LOC396098 (Chicken)
Gene ID Aliases B6.2; Bu-1; Bu-1b

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

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Other Conjugates and Formulations

Citations

  1. 1. Veromaa T, Vainio O, Eerola E, Toivanen P. Monoclonal antibodies against chicken Bu-1a and Bu-1b alloantigens. Hybridoma. 1988;7:41-8. (Immunogen, ICC, FC)
  2. 2. Nagy N, Bíró É, Takács Á, Pólos M, Magyar A, Oláh I. Peripheral blood fibrocytes contribute to the formation of the avian spleen. Dev Dyn. 2005;232:55-66. (IHC-FS)
  3. 3. Felföldi B, Imre G, Igyártó B, Iván J, Mihalik R, Lackó E, et al. In ovo vitelline duct ligation results in transient changes of bursal microenvironments. Immunology. 2005;116:267-75. (IHC-FS)
  4. 4. Kabell S, Igyártó B, Magyar A, Hajdú Z, Bíró É, Bisgaard M, et al. Impact of heterophil granulocyte depletion caused by 5-fluorouracil on infectious bursal disease virus infection in specific pathogen free chickens. Avian Pathol. 2006;35:341-8. (IHC-FS)
  5. 5. Igyártó B, Magyar A, Oláh I. Origin of follicular dendritic cell in the chicken spleen. Cell Tissue Res. 2007;327:83-92. (IHC-FS)
  6. 6. Igyártó BZ, Nagy N, Magyar A, Oláh I. Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody. Poult Sci. 2008;87:351-5. (IHC-FS)
  7. 7. Li X, Rees JS, Xue P, Zhang H, Hamaia SW, Sanderson B, et al. New insights into the DT40 B cell receptor cluster using a proteomic proximity labeling assay. J Biol Chem. 2014;289:14434-47. (IP, ICC)
  8. 8. Funk PE, Pifer J, Kharas M, Crisafi G, Jonhnson A. The avian chB6 alloantigen induces apoptosis in DT40 B cells. Cell Immunol. 2003;226:95-104. (Apop, IP)
  9. 9. Narvi E, Nera K, Terho P, Mustonen L, Granberg J, Lassila O. Aiolos controls gene conversion and cell death in DT40 B cells. Scand J Immunol. 2007;65:503-13. (Apop)
  10. 10. Huffnagle GB, Ratcliffe MJ, Humphries EH. Bu-2, a novel avian cell surface antigen on B cells and a population of non-lymphoid cells, is expressed homogeneously in germinal centers. Hybridoma. 1989;8:589-604. (FC)
  11. 11. Ratcliffe MJ. Generation of immunoglobulin heavy chain diversity subsequent to cell surface immunoglobulin expression in the avian bursa of Fabricius. J Exp Med. 1989;170:1165-73. (FC)
  12. 12. Pararnithiotis E, Jacobsen KA, Ratcliffe MJ. Loss of surface immunoglobulin expression precedes B cell death by apoptosis in the bursa of Fabricius. J Exp Med. 1995;181:105-13. (FC)
  13. 13. Sayegh CE, Demaries SL, Iacampo S, Ratcliffe MJ. Development of B cells expressing surface immunoglobulin molecules that lack V(D)J-encoded determinants in the avian embryo bursa of fabricius. Proc Natl Acad Sci USA. 1999;96:10806-11. (FC)
  14. 14. Schulte CE, von Lindern M, Steinlein P, Beug H, Wiedemann LM. MLL-ENL cooperates with SCF to transform primary avian multipotent cells. EMBO J. 2002;21:4297-306. (FC)
  15. 15. Hunt HD, Goto RM, Foster DN, Bacon LD, Miller MM. At least one YMHCI molecule in the chicken is alloimmunogenic and dynamically expressed on spleen cells during development. Immunogenetics. 2006;58:297-307. (FC)
  16. 16. Petkov DI, Linnemann EG, Kapczynski DR, Sellers HS. Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens. Avian Dis. 2009;53:347-55. (FC)
See All References

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