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Mouse Anti-Chicken Bu-1a-PE (21-1A4)
Cat. No.:
8365-09
PE Anti-Chicken Bu-1a antibody for use in flow cytometry assays.
$237.00
| Clone | 21-1A4 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-PE (15H6) |
| Specificity | Chicken Bu-1a |
| Alternative Names | ChB6.1 |
| Description | Chicken Bu-1a, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 21-1A4 does not react with cells from CHA and H.B14ab strains by immunofluorescence. |
| Immunogen | Bursa cells from one-day-old H.B15 (Bu-1a/b) strain chickens |
| Conjugate | PE (R-phycoerythrin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
| Clonality | Monoclonal |
| Concentration | 0.1 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
| Applications |
Flow Cytometry – Quality tested 1,5-9 Immunohistochemistry-Frozen Sections – Reported in literature 2 Immunocytochemistry – Reported in literature 1 Immunoprecipitation – Reported in literature 3,4 Apoptosis – Reported in literature 4 |
| RRID Number | AB_2796524 |
| Gene ID |
396098 (Chicken) |
| Gene ID Symbol |
LOC396098 (Chicken) |
| Gene ID Aliases | B6.2; Bu-1; Bu-1b |
Documentation
Certificate of Analysis Lookup
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- 1. Veromaa T, Vainio O, Eerola E, Toivanen P. Monoclonal antibodies against chicken Bu-1a and Bu-1b alloantigens. Hybridoma. 1988;7:41-8. (Immunogen, ICC, FC)
- 2. Sayegh CE, Ratcliffe MJ. Perinatal deletion of B cells expressing surface Ig molecules that lack V(D)J-encoded determinants in the bursa of Fabricius is not due to intrafollicular competition. J Immunol. 2000;164:5041-8. (IHC-FS)
- 3. Huffnagle GB, Ratcliffe MJ, Humphries EH. Bu-2, a novel avian cell surface antigen on B cells and a population of non-lymphoid cells, is expressed homogeneously in germinal centers. Hybridoma. 1989;8:589-604. (FC, IP)
- 4. Funk PE, Pifer J, Kharas M, Crisafi G, Johnson A. The avian chB6 alloantigen induces apoptosis in DT40 B cells. Cell Immunol. 2003;226:95-104. (IP, Apop)
- 5. Ratcliffe MJ. Generation of immunoglobulin heavy chain diversity subsequent to cell surface immunoglobulin expression in the avian bursa of Fabricius. J Exp Med. 1989;170:1165-73. (FC)
- 6. Pararnithiotis E, Jacobsen KA, Ratcliffe MJ. Loss of surface immunoglobulin expression precedes B cell death by apoptosis in the bursa of Fabricius. J Exp Med. 1995;181:105-13. (FC)
- 7. Sayegh CE, Demaries SL, Iacampo S, Ratcliffe MJ. Development of B cells expressing surface immunoglobulin molecules that lack V(D)J-encoded determinants in the avian embryo bursa of fabricius. Proc Natl Acad Sci USA. 1999;96:10806-11. (FC)
- 8. Hunt HD, Goto RM, Foster DN, Bacon LD, Miller MM. At least one YMHCI molecule in the chicken is alloimmunogenic and dynamically expressed on spleen cells during development. Immunogenetics. 2006;58:297-307. (FC)
- 9. Alitheen NB, McClure SJ, Yeap SK, Kristeen-Teo YW, Tan SW, McCullagh P. Establishment of an in vitro system representing the chicken gut-associated lymphoid tissue. PLoS One. 2012;7(11):e49188. (FC)
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