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Mouse Anti-Chicken β2-Microglobulin-PE (F21-21)
Cat. No.:
8355-09
PE Anti-Chicken β2 antibody for use in flow cytometry assays.
$237.00
| Clone | F21-21 |
|---|---|
| Isotype | Mouse IgG1κ |
| Isotype Control | Mouse IgG1-PE (15H6) |
| Specificity | Chicken/Turkey β2-Microglobulin |
| Alternative Names | MHC Class I, B-F |
| Description | Like their mammalian counterparts, avian MHC Class I molecules, also known as B-F antigens, consist of a highly polymorphic α-chain noncovalently bound to the invariant β2-microglobulin subunit. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes which are usually cytotoxic T cells. The monoclonal antibody F21-21 also reacts with turkey β2-microglobulin. |
| Immunogen | Affinity purified chicken MHC-I molecules |
| Conjugate | PE (R-phycoerythrin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
| Clonality | Monoclonal |
| Concentration | 0.1 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
| Applications |
Flow Cytometry – Quality tested 4,6-10 Immunocytochemistry – Reported in literature 3 Immunoprecipitation – Reported in literature 5,6 Western Blot – Reported in literature 1,2 Purification – Reported in literature 1,4,6 |
| RRID Number | AB_2796520 |
| Gene ID |
414830 (Chicken) 100539873 (Turkey) |
| Gene ID Symbol |
B2M (Chicken) B2M (Turkey) |
| Gene ID Aliases | b2m-W01; b2m-W03; b2m-Z01; b2m-Z02; b2m-Z03 |
| UniProt ID |
P21611 (Chicken P21612 (Turkey |
| UniProt Name |
B2MG_CHICK (Chicken) B2MG_MELGA (Turkey) |
Documentation
Certificate of Analysis Lookup
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- 1. Skjødt K, Welinder KG, Crone M, Verland S, Salomonsen J, Simonsen M. Isolation and characterization of chicken and turkey beta2-microglobulin. Mol Immunol. 1986;23:1301-9. (Immunogen, WB, Purification, Turkey Reactivity)
- 2. Walker BA, Hunt LG, Sowa AK, Skjødt K, Göbel TW, Lehner PJ, et al. The dominantly expressed class I molecule of the chicken MHC is explained by coevolution with the polymorphic peptide transporter (TAP) genes. Proc Natl Acad Sci USA. 2011;108:8396-401. (WB)
- 3. Adair BM, McNeilly F, McConnell CD, McNulty MS. Characterization of surface markers present on cells infected by chicken anemia virus in experimentally infected chickens. Avian Dis. 1993;37:943-50. (ICC)
- 4. Dunon D, Kaufman J, Salomonsen J, Skjoedt K, Vainio O, Thiery J, et al. T cell precursor migration towards β2-microglobulin is involved in thymus colonization of chicken embryos. EMBO J. 1990;9:3315-22. (Purification, FC)
- 5. Møller LB, Kaufman J, Verland S, Salomonsen J, Avila D, Lambris JD, et al. Variations in the cytoplasmic region account for the heterogeneity of the chicken MHC class I (B-F) molecules. Immunogenetics. 1991;34:110-20. (IP)
- 6. Wallny H, Avila D, Hunt LG, Powell TJ, Riegert P, Salomonsen J, et al. Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens. Proc Natl Acad Sci USA. 2006;103:1434-9. (FC, IP, Purification)
- 7. Buitenhuis AJ, Kjaer JB, Labouriau R, Juul-Madsen HR. Altered circulating levels of serotonin and immunological changes in laying hens divergently selected for feather pecking behavior. Poult Sci. 2006;85:1722-8. (FC)
- 8. Juul-Madsen HR, Dalgaard TS, Røntved CM, Jensen KH, Bumstead N. Immune response to a killed infectious bursal disease virus vaccine in inbred chicken lines with different major histocompatibility complex haplotypes. Poult Sci. 2006;85:986-98. (FC)
- 9. Dalgaard T, Boving MK, Handberg K, Jensen KH, Norup LR, Juul-Madsen HR. MHC expression on spleen lymphocyte subsets in genetically resistant and susceptible chickens infected with Marek's disease virus. Viral Immunol. 2009;22:321-7. (FC)
- 10. Meyerhoff RR, Ali RA, Liu K, Huang G, Koci MD. Comprehensive analysis of commercially available mouse antichicken monoclonal antibodies for cross-reactivity with peripheral blood leukocytes from commercial turkeys. Poult Sci. 2012;91:383-92. (FC, Turkey Reactivity)
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