Mouse Anti-Biotin-PE (SB58c)
Cat. No.:
6404-09
PE Anti-Biotin antibody for use in flow cytometry assays.
$182.00
![BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45R-BIOT (SB Cat. No. 1665-08) followed by Mouse Anti-Biotin-PE (SB Cat. No. 6404-09).](https://www.southernbiotech.com/media/catalog/product/cache/7149a3d30fe6da616557c86e375dcb18/3/e/3eecd4c64e347b3ed8403de63796033f8d2a9fcbdfe5c7ff48e0b916f91f2d27.jpeg)
![BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45R-BIOT (SB Cat. No. 1665-08) followed by Mouse Anti-Biotin-PE (SB Cat. No. 6404-09).](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/3/e/3eecd4c64e347b3ed8403de63796033f8d2a9fcbdfe5c7ff48e0b916f91f2d27.jpeg)
![FLISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>2b</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Mouse Anti-Biotin-PE (SB Cat. No. 6404-09).](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/f/4/f418aa2c9caf7d44846b56674e111a62d59236675fa213a9b375e8ac898d63d1.jpeg)
Clone | SB58c |
---|---|
Isotype | Mouse (BALB/c) IgG1κ |
Isotype Control | Mouse IgG1-PE (15H6) |
Specificity | Biotin |
Description | Anti-Biotin may be used for indirect immunostaining when working with a biotinylated primary antibody. As an effective alternative to avidin, Anti-Biotin amplifies signal and reduces background that can be caused by avidin and its derivatives. |
Immunogen | Biotin-KLH |
Conjugate | PE (R-phycoerythrin) |
Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
Clonality | Monoclonal |
Concentration | 0.5 mg/mL |
Volume | 0.2 mL |
Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
Applications |
ELISA – Quality tested FLISA – Quality tested Flow Cytometry – Quality tested Western Blot – Reported in literature 1 Proximity Ligation Assay – Reported in literature 2 |
RRID Number | AB_2796300 |
Documentation
Certificate of Analysis Lookup
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- 1. Späte A, Schart VF, Häfner J, Niederwieser A, Mayer TU, Wittmann V. Expanding the scope of cyclopropene reporters for the detection of metabolically engineered glycoproteins by Diels-Alder reactions. Beilstein J Org Chem. 2014;10:2235-42. (WB)
- 2. Workman ER, Haddick PC, Bush K, Dilly GA, Niere F, Zemelman BV, et al. Rapid antidepressants stimulate the decoupling of GABAB receptors from GIRK/Kir3 channels through increased protein stability of 14-3-3η. Mol Psychiatry. 2015;20:298-310. (Proximity ligation assay)
- 3. Fahie MA, Yang B, Mullis M, Holden MA, Chen M. Selective detection of protein homologues in serum using an OmpG nanopore. Anal Chem. 2015;87:11143-9.