Mouse Anti-Biotin-HRP (SB58c)
Cat. No.:
6404-05
HRP Anti-Biotin antibody for use in ELISA and western blot assays.
$204.00
![Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (SB Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05) secondary antibody and chemiluminescent detection.](https://www.southernbiotech.com/media/catalog/product/cache/7149a3d30fe6da616557c86e375dcb18/1/4/14c8a4305c86191aee0041e01658b64f1e41aa335b400a58748f694abf5c0b90.jpeg)
![Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (SB Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05) secondary antibody and chemiluminescent detection.](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/1/4/14c8a4305c86191aee0041e01658b64f1e41aa335b400a58748f694abf5c0b90.jpeg)
![ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>2b</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Mouse Anti-Biotin-HRP (SB Cat. No. 6404-05).](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/6/3/63b019b8d7c3312a81bb3a1ffe1f85a4eb1361aee03e2ebfc4d5ff1800900140.jpeg)
Clone | SB58c |
---|---|
Isotype | Mouse (BALB/c) IgG1κ |
Isotype Control | Mouse IgG1-HRP (15H6) |
Specificity | Biotin |
Description | Anti-Biotin may be used for indirect immunostaining when working with a biotinylated primary antibody. As an effective alternative to avidin, Anti-Biotin amplifies signal and reduces background that can be caused by avidin and its derivatives. |
Immunogen | Biotin-KLH |
Conjugate | HRP (Horseradish Peroxidase) |
Buffer Formulation | 50% Glycerol/50% Phosphate buffered saline, pH 7.4 |
Clonality | Monoclonal |
Concentration | Lot specific |
Volume | 1.0 mL |
Recommended Storage | 2-8°C |
Applications |
ELISA – Quality tested FLISA – Quality tested Flow Cytometry – Quality tested Western Blot – Reported in literature 1 Proximity Ligation Assay – Reported in literature 2 |
RRID Number | AB_2796299 |
Documentation
Certificate of Analysis Lookup
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- 1. Späte A, Schart VF, Häfner J, Niederwieser A, Mayer TU, Wittmann V. Expanding the scope of cyclopropene reporters for the detection of metabolically engineered glycoproteins by Diels-Alder reactions. Beilstein J Org Chem. 2014;10:2235-42. (WB)
- 2. Workman ER, Haddick PC, Bush K, Dilly GA, Niere F, Zemelman BV, et al. Rapid antidepressants stimulate the decoupling of GABAB receptors from GIRK/Kir3 channels through increased protein stability of 14-3-3η. Mol Psychiatry. 2015;20:298-310. (Proximity ligation assay)
- 3. Fahie MA, Yang B, Mullis M, Holden MA, Chen M. Selective detection of protein homologues in serum using an OmpG nanopore. Anal Chem. 2015;87:11143-9.