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Hamster Anti-Mouse CD81-LE/AF (2F7)
Cat. No.:
1825-14
Low Endotoxin/Azide Free Anti-Mouse CD81 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, costimulation, and blocking assays.
$292.00
| Clone | 2F7 |
|---|---|
| Isotype | Hamster (Armenian) IgG3 |
| Isotype Control | Hamster IgG-LE/AF |
| Specificity | Mouse CD81 |
| Alternative Names | TAPA-1 |
| Description | CD81, also known as TAPA-1, is an integral membrane protein expressed on a variety of cell types and has a high degree of sequence homology between human and mouse. CD81 is expressed on thymic stromal cells where it plays an important role in the transition of γδ+ T cells to more mature T cells with αβ T cell receptors. Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor which is composed of a TCR β chain and glycoprotein pTα. The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro. |
| Immunogen | Mouse epithelial cell line PAM212 |
| Conjugate | LE/AF (Low Endotoxin/Azide Free) |
| Buffer Formulation | Phosphate buffered saline, pH 7.4 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Handle under aseptic conditions |
| Applications | Flow Cytometry – Quality tested 1,3 Immunohistochemistry –Reported in literature 1 Immunocytochemistry – Reported in literature 5 Immunoprecipitation – Reported in literature 1 Blocking – Reported in literature 1-4 Costimulation – Reported in literature 2 |
| RRID Number | AB_2795403 |
| Gene ID | 12520 (Mouse) |
| Gene ID Symbol | Cd81 (Mouse) |
| Gene ID Aliases | Tapa-1; Tapa1; Tspan28 |
| UniProt ID | P35762 (Mouse |
| UniProt Name | CD81_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Boismenu R, Rhein M, Fischer WH, Havran WL. A role for CD81 in early T cell development. Science. 1996;271:198-200. (Immunogen, FC, IP, IHC, Block)
- 2. Takeda Y, He P, Tachibana I, Zhou B, Miyado K, Kaneko H, et al. Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice. J Biol Chem. 2008;283:26089-97. (Block, Costim)
- 3. Tachibana I, Hemler ME. Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance. J Cell Biol. 1999;146:893-904. (FC, Block)
- 4. Chang Y, Finnemann SC. Tetraspanin CD81 is required for the αvβ5-integrin-dependent particle-binding step of RPE phagocytosis. J Cell Sci. 2007;120:3053-63. (Block)
- 5. Kelić S, Levy S, Suarez C, Weinstein DE. CD81 regulates neuron-induced astrocyte cell-cycle exit. Mol Cell Neurosci. 2001;17:551-60. (ICC)
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