Goat F(ab')2 IgG-UNLB
Cat. No.:
0110-01
Purified Goat F(ab')2 IgG for use as a control in flow cytometry and immunocytochemistry assays and as a standard in ELISA.
$191.00
![ELISA plate was coated with serially diluted Goat F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0110-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) and Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.](https://www.southernbiotech.com/media/catalog/product/cache/7149a3d30fe6da616557c86e375dcb18/f/4/f462f30e4c455d9990240a6f326db31314b5e70343d2484e23be1e4c96052925.jpeg)
![ELISA plate was coated with serially diluted Goat F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0110-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) and Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/f/4/f462f30e4c455d9990240a6f326db31314b5e70343d2484e23be1e4c96052925.jpeg)
![Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0110-01; gray) and Goat F(ab')<sub>2</sub> Anti-Mouse Ig, Human ads-UNLB (SB Cat. No. 1012-01) then Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-FITC (SB Cat. No. 6300-02).](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/2/e/2e8dc05ad9d40fa1a5b7ac5289457a45e7a7559c710ec2400ac4b924d7343587.jpeg)
Isotype | Goat F(ab')2 IgG |
---|---|
Source | Pepsin digest of Goat IgG (SB Cat. No. 0109) |
Conjugate | UNLB (Unconjugated) |
Buffer Formulation | Borate buffered saline, pH 8.2 |
Clonality | Polyclonal |
Concentration | 5 mg/mL |
Volume | 1.0 mL |
Recommended Storage | 2-8°C |
Applications |
Quality tested applications for relevant formats include - Flow Cytometry 1-3 ELISA FLISA Other referenced applications for relevant formats include - Immunocytochemistry 4 |
RRID Number | AB_2793956 |
Documentation
Certificate of Analysis Lookup
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- 1. Coccia MA, Brams P. High titer, prostate specific antigen-specific human IgG production by hu-PBL-SCID mice immunized with antigen-mouse IgG2a complex-pulsed autologous dendritic cells. J Immunol. 1998;161:5772-80. (FC)
- 2. Delaleu N, Immervoll H, Cornelius J, Jonsson R. Biomarker profiles in serum and saliva of experimental Sjögren's syndrome: associations with specific autoimmune manifestations. Arthritis Res Ther. 2008;10:R22. (FC)
- 3. Delaleu N, Madureira AC, Immervoll H, Jonsson R. Inhibition of experimental Sjögren's syndrome through immunization with HSP60 and its peptide amino acids 437-460. Arthritis Rheum. 2008;58:2318-28. (FC)
- 4. Montalvão F, Almeida GM, Silva EM, Borges VM, Vasconcellos R, Takiya CM, et al. Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-α secretion and reduce parasite replication in macrophages. Eur J Immunol. 2010;40:417-25. (ICC)
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