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Goat F(ab')2 IgG-UNLB

Cat. No.:
0110-01
Purified Goat F(ab')2 IgG for use as a control in flow cytometry and immunocytochemistry assays and as a standard in ELISA.
$191.00
Size Price (USD) Quantity
5.0 mg $191.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat F(ab')2 IgG
Source Pepsin digest of Goat IgG (SB Cat. No. 0109)
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
Flow Cytometry 1-3
ELISA
FLISA

Other referenced applications for relevant formats include -
Immunocytochemistry 4
RRID Number AB_2793956

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Coccia MA, Brams P. High titer, prostate specific antigen-specific human IgG production by hu-PBL-SCID mice immunized with antigen-mouse IgG2a complex-pulsed autologous dendritic cells. J Immunol. 1998;161:5772-80. (FC)
  2. 2. Delaleu N, Immervoll H, Cornelius J, Jonsson R. Biomarker profiles in serum and saliva of experimental Sjögren's syndrome: associations with specific autoimmune manifestations. Arthritis Res Ther. 2008;10:R22. (FC)
  3. 3. Delaleu N, Madureira AC, Immervoll H, Jonsson R. Inhibition of experimental Sjögren's syndrome through immunization with HSP60 and its peptide amino acids 437-460. Arthritis Rheum. 2008;58:2318-28. (FC)
  4. 4. Montalvão F, Almeida GM, Silva EM, Borges VM, Vasconcellos R, Takiya CM, et al. Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-α secretion and reduce parasite replication in macrophages. Eur J Immunol. 2010;40:417-25. (ICC)
See All References

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  2. Ramos cell lysates treated with HM71224 and stimulated with Goat F(ab')<sub>2</sub> Anti-Human IgM-UNLB (SB Cat. No. 2022-01) were resolved by electrophoresis, transferred to PVDF membrane, and probed with anti-phospho-PLCγ2Y1217 followed by an HRP conjugated secondary antibody and chemiluminescent detection.<br/>Image from Kim Y, Park KT, Jang SY, Lee KH, Byun J, Suh KH, et al. HM71224, a selective Bruton's tyrosine kinase inhibitor, attenuates the development of murine lupus. Arthritis Res Ther. 2017;19:211. Figure 1(a)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  4. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human IgG-UNLB (SB Cat. No. 2042-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  5. ELISA plate was coated with purified rat IgG and IgM and mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rat IgG(H+L), Mouse ads-UNLB (SB Cat. No. 3052-01) followed by Swine Anti-Goat IgG(H+L), Human/ Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  6. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  7. ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Mouse IgM-UNLB (SB Cat. No. 1023-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  9. Paraffin embedded mouse spleen section was stained with Goat F(ab')<sub>2</sub> Anti-Mouse Kappa-UNLB (SB Cat. No. 1052-01) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AP (SB Cat. No. 6420-04), Red AP, and hematoxylin.
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