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Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT

Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT

Name: Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT
SKU: 4052-08
Price: 94 USD
Availability: InStock

Cat. No.:

4052-08

Goat F(ab')₂ Anti-Rabbit IgG(H+L)-Biotin antibody with minimal reactivity to mouse and human proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, and western blot assays.

$94.00

Size	Price (USD)	Quantity
0.5 mg	$94.00

Qty

Technical Bulletin Safety Datasheet

ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4052-08) followed Streptavidin-HRP (SB Cat. No. 7100-05).

ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')₂ Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4052-08) followed Streptavidin-HRP (SB Cat. No. 7100-05).

Product Specifications

More Information
Isotype	Goat F(ab')₂ IgG
Isotype Control	Goat F(ab')2 IgG-BIOT
Specificity	Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM
Source	Pepsin digest of Goat Anti-Rabbit IgG(H+L), Mouse/Human ads (SB Cat. No. 4050)
Cross Adsorption	Mouse and human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Conjugate	BIOT (Biotin)
Buffer Formulation	Phosphate buffered saline containing < 0.1% sodium azide
Clonality	Polyclonal
Concentration	0.5 mg/mL
Volume	1.0 mL
Recommended Storage	2-8°C
Applications	Quality tested applications for relevant formats include - ELISA ^1,2 FLISA Flow Cytometry ^3-10 Other referenced applications for relevant formats include - Immunohistochemistry-Frozen Sections ^11-14 Immunohistochemistry-Paraffin Sections ^15-17 Imuunocytochemistry ^6,9,14,18-20 Western Blot ^21-24
RRID Number	AB_2795970

Documentation

Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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Other Conjugates and Formulations

Citations

1. Pilling D, Buckley CD, Salmon M, Gomer RH. Inhibition of fibrocyte differentiation by serum amyloid P. J Immunol. 2003;171:5537-46. (ELISA)
2. Koehler NK, Stransky E, Meyer M, Gaertner S, Shing M, Schnaidt M, et al. Alpha-synuclein levels in blood plasma decline with healthy aging. PLoS One. 2015;10(4):e0123444. (ELISA)
3. Kumar S, Tarleton RL. Antigen-specific Th1 but not Th2 cells provide protection from lethal Trypanosoma cruzi infection in mice. J Immunol. 2001;166:4596-603. (FC)
4. Xi H, Kersh GJ. Sustained early growth response gene 3 expression inhibits the survival of CD4/CD8 double-positive thymocytes. J Immunol. 2004;173:340-8. (FC)
5. Fulton BE Jr, Portella M, Radke K. Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node. J Virol. 2006;80:7873-84. (FC)
6. Flores-Borja F, Kabouridis PS, Jury EC, Isenberg DA, Mageed RA. Altered lipid raft-associated proximal signaling and translocation of CD45 tyrosine phosphatase in B lymphocytes from patients with systemic lupus erythematosus. Arthritis Rheum. 2007;56:291-302. (FC, ICC)
7. Espinassous Q, Garcia-de-Paco E, Garcia-Verdugo I, Synguelakis M, von Aulock S, Sallenave J, et al. IL-33 enhances lipopolysaccharide-induced inflammatory cytokine production from mouse macrophages by regulating lipopolysaccharide receptor complex. J Immunol. 2009;183:1446-55. (FC)
8. Zhou Q, Yan J, Putheti P, Wu Y, Sun X, Toxavidis V, et al. Isolated CD39 expression on CD4⁺ T cells denotes both regulatory and memory populations. Am J Transplant. 2009;9:2303-11. (FC)
9. Crawford JR, Pilling D, Gomer RH. Improved serum-free culture conditions for spleen-derived murine fibrocytes. J Immunol Methods. 2010;363:9-20. (FC, ICC)
10. Le Gall SM, Legrand J, Benbijja M, Safya H, Benihoud K, Kanellopoulos JM, et al. Loss of P2X7 receptor plasma membrane expression and function in pathogenic B220⁺ double-negative T lymphocytes of autoimmune MRL/lpr mice. PLoS One. 2012;7(12):e52161. (FC)
11. Heeringa P, Van den Born J, Brouwer E, Dolman KM, Klok PA, Huitema MG, et al. Elastase, but not proteinase 3 (PR3), induces proteinuria associated with loss of glomerular basement membrane heparan sulphate after in vivo renal perfusion in rats. Clin Exp Immunol. 1996;105:321-9. (IHC-FS)
12. Pilling D, Roife D, Wang M, Ronkainen SD, Crawford JR, Travis EL, et al. Reduction of bleomycin-induced pulmonary fibrosis by serum amyloid P. J Immunol. 2007;179:4035-44. (IHC-FS)
13. Gomer RH, Pilling D, Kauvar LM, Ellsworth S, Ronkainen SD, Roife D, et al. A serum amyloid P-binding hydrogel speeds healing of partial thickness wounds in pigs. Wound Repair Regen. 2009;17:397-404. (IHC-FS)
14. Knight JS, Zhao W, Luo W, Subramanian V, O'Dell AA, Yalavarthi S, et al. Peptidylarginine deiminase inhibition is immunomodulatory and vasculoprotective in murine lupus. J Clin Invest. 2013;123:2981-93. (IHC-FS, ICC)
15. van Ginkel FW, McGhee JR, Watt JM, Campos-Torres A, Parish LA, Briles DE. Pneumococcal carriage results in ganglioside-mediated olfactory tissue infection. Proc Natl Acad Sci USA. 2003;100:14363-7. (IHC-PS)
16. Ludvigsen E, Olsson R, Stridsberg M, Janson ET, Sandler S. Expression and distribution of somatostatin receptor subtypes in the pancreatic islets of mice and rats. J Histochem Cytochem. 2004;52:391-400. (IHC-PS)
17. Hassounah NB, Nagle R, Saboda K, Roe DJ, Dalkin BL, McDermott KM. Primary cilia are lost in preinvasive and invasive prostate cancer. PLoS One. 2013;8(7):e68521. (IHC-PS)
18. Rasmussen UB, Schreiber V, Schultz H, Mischler F, Schughart K. Tumor cell-targeting by phage-displayed peptides. Cancer Gene Ther. 2002;9:606-12. (ICC)
19. Shao DD, Suresh R, Vakil V, Gomer RH, Pilling D. Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation. J Leukoc Biol. 2008;83:1323-33. (ICC)
20. Crawford JR, Pilling D, Gomer RH. FcγRI mediates serum amyloid P inhibition of fibrocyte differentiation. J Leukoc Biol. 2012;92:699-711. (ICC)
21. Perez-Polo JR, Reilly CB, Rea HC. Oxygen resuscitation after hypoxia ischemia stimulates prostaglandin pathway in rat cortex. Int J Dev Neurosci. 2011;29:639-44. (WB)
22. King RG, Herrin BR, Justement LB. Differential expression of the adaptor protein HSH2 controls the quantitative and qualitative nature of the humoral response. J Immunol. 2011;187:3565-77. (WB)
23. Lee-Durham JC, Wu Y, Mokkapati VU, Paulucci-Holthauzen AA, Nesic O. Induction of angiopoietin-2 after spinal cord injury. Neuroscience. 2012;202:454-64. (WB)
24. Schröder W, Goerke C, Wolz C. Opposing effects of aminocoumarins and fluoroquinolones on the SOS response and adaptability in Staphylococcus aureus. J Antimicrob Chemother. 2013;68:529-38. (WB)