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  • Goat F(ab')2 Anti-Mouse IgM, Human ads-AF555

Goat F(ab')2 Anti-Mouse IgM, Human ads-AF555

Cat. No.:
1022-32
Goat F(ab')2 Anti-Mouse IgM-Alexa Fluor® 555 antibody with minimal reactivity to human proteins for use in immunohistochemistry / immunocytochemistry assays.
$202.00
Size Price (USD) Quantity
0.5 mg $202.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat F(ab')2 IgG
Specificity Reacts with the heavy chain of mouse IgM
Source Pepsin digest of Goat Anti-Mouse IgM, Human ads (SB Cat. No. 1020)
Cross Adsorption Mouse IgG and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Conjugate AF555 (Alexa Fluor® 555)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA 1
FLISA
Flow Cytometry 2-7

Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 8
Immunohistochemistry-Paraffin Sections 9
Immunocytochemistry 10,11
Stimulation 12-25
Purification 26
RRID Number AB_2794269
Gene ID 16019 (Mouse)
Gene ID Symbol Ighm (Mouse)
Gene ID Aliases AI326478; Igh-6; Igh-M; Igh6; Igm; muH
UniProt ID P01872 (Mouse
UniProt Name IGHM_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Kang M, Lu H, Yasui T, Sharpe A, Warren H, Cahir-McFarland E, et al. Epstein-Barr virus nuclear antigen 1 does not induce lymphoma in transgenic FVB mice. Proc Natl Acad Sci USA. 2005;102:820-5. (ELISA)
  2. 2. Yan J, Vetvicka V, Xia Y, Coxon A, Carroll MC, Mayadas TN, et al. β-glucan, a "specific" biologic response modifier that uses antibodies to target tumors for cytotoxic recognition by leukocyte complement receptor type 3 (CD11b/CD18). J Immunol. 1999;163:3045-52. (FC)
  3. 3. Stabel TJ, Bolin SR, Pesch BA, Rahner TE. A simple and rapid flow cytometric method for detection of porcine cell surface markers. J Immunol Methods. 2000;245:147-52. (FC)
  4. 4. de Baey A, Mende I, Baretton G, Greiner A, Hartl WH, Baeuerle PA, et al. A subset of human dendritic cells in the T cell area of mucosa-associated lymphoid tissue with a high potential to produce TNF-α. J Immunol. 2003;170:5089-94. (FC)
  5. 5. Wallace DL, Zhang Y, Ghattas H, Worth A, Irvine A, Bennett AR, et al. Direct measurement of T cell subset kinetics in vivo in elderly men and women. J Immunol. 2004;173:1787-94. (FC)
  6. 6. Hoffman LM, Hall L, Batten JL, Young H, Pardasani D, Baetge EE, et al. X-inactivation status varies in human embryonic stem cell lines. Stem Cells. 2005;23:1468-78. (FC)
  7. 7. Guikema JE, Schrader CE, Leus NG, Ucher A, Linehan EK, Werling U, et al. Reassessment of the role of Mut S homolog 5 in Ig class switch recombination shows lack of involvement in cis- and trans-switching. J Immunol. 2008;181:8450-9. (FC)
  8. 8. Geraldes P, Rebrovich M, Herrmann K, Wong J, Jäck H, Wabl M, et al. Ig heavy chain promotes mature B cell survival in the absence of light chain. J Immunol. 2007;179:1659-68. (IHC-FS)
  9. 9. van Ginkel FW, Wahl SM, Kearney JF, Kweon M, Fujihashi K, Burrows PD, et al. Partial IgA-deficiency with increased Th2-type cytokines in TGF-β1 knockout mice. J Immunol. 1999;163:1951-7. (IHC-PS)
  10. 10. Montalvão F, Almeida GM, Silva EM, Borges VM, Vasconcellos R, Takiya CM, et al. Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-α secretion and reduce parasite replication in macrophages. Eur J Immunol. 2010;40:417-25. (ICC)
  11. 11. Miyazaki A, Yogosawa S, Murakami A, Kitamura D. Identification of CMTM7 as a transmembrane linker of BLNK and the B-cell receptor. PLoS One. 2012;7(2):e31829. (ICC)
  12. 12. Takeshita H, Taniuchi I, Kato J, Watanabe T. Abrogation of autoimmune disease in Lyn-deficient mice by the mutation of the Btk gene. Int Immunol. 1998;10:435-44. (Stim)
  13. 13. Oliver AM, Martin F, Kearney JF. IgMhighCD21high lymphocytes enriched in the splenic marginal zone generate effector cells more rapidly than the bulk of follicular B cells. J Immunol. 1999;162:7198-207. (Stim)
  14. 14. Ochi H, Watanabe T. Negative regulation of B cell receptor-mediated signaling in B-1 cells through CD5 and Ly49 co-receptors via Lyn kinase activity. Int Immunol. 2000;12:1417-23. (Stim)
  15. 15. Kato I, Takai T, Kudo A. The pre-B cell receptor signaling for apoptosis is negatively regulated by FcγRIIB. J Immunol. 2002;168:629-34. (Stim)
  16. 16. Gavin AL, Aït-Azzouzene D, Ware CF, Nemazee D. ΔBAFF, an alternate splice isoform that regulates receptor binding and biopresentation of the B cell survival cytokine, BAFF. J Biol Chem. 2003;278:38220-8. (Stim)
  17. 17. Chattopadhyay G, Khan AQ, Sen G, Colino J, duBois W, Rubtsov A, et al. Transgenic expression of Bcl-xL or Bcl-2 by murine B cells enhances the in vivo antipolysaccharide, but not antiprotein, response to intact Streptococcus pneumoniae. J Immunol. 2007;179:7523-34. (Stim)
  18. 18. Bahjat FR, Pine PR, Reitsma A, Cassafer G, Baluom M, Grillo S, et al. An orally bioavailable spleen tyrosine kinase inhibitor delays disease progression and prolongs survival in murine lupus. Arthritis Rheum. 2008;58:1433-44. (Stim)
  19. 19. Hauser J, Verma-Gaur J, Wallenius A, Grundström T. Initiation of antigen receptor-dependent differentiation into plasma cells by calmodulin inhibition of E2A. J Immunol. 2009;183:1179-87. (Stim)
  20. 20. Woo A, Kim J, Jeong Y, Maeng HG, Lee Y, Kang JS, et al. Vitamin C acts indirectly to modulate isotype switching in mouse B cells. Anat Cell Biol. 2010;43:25-35. (Stim)
  21. 21. Hauser J, Grundström C, Grundström T. Allelic exclusion of IgH through inhibition of E2A in a VDJ recombination complex. J Immunol. 2014;192:2460-70. (Stim)
  22. 22. Lindner JM, Kayo H, Hedlund S, Fukuda Y, Fukao T, Nielsen PJ. Cutting edge: The transcription factor Bob1 counteracts B cell activation and regulates miR-146a in B cells. J Immunol. 2014;192:4483-6. (Stim)
  23. 23. Goverse G, Olivier BJ, Molenaar R, Knippenberg M, Greuter M, Konijn T, et al. Vitamin A metabolism and mucosal immune function are distinct between BALB/c and C57BL/6 mice. Eur J Immunol. 2015;45:89-100. (Stim)
  24. 24. Hu J, Oda SK, Shotts K, Donovan EE, Strauch P, Pujanauski LM, et al. Lysophosphatidic acid receptor 5 inhibits B cell antigen receptor signaling and antibody response. J Immunol. 2014;193:85-95. (Stim)
  25. 25. Bestas B, Moreno PM, Blomberg KE, Mohammad DK, Saleh AF, Sutlu T, et al. Splice-correcting oligonucleotides restore BTK function in X-linked agammaglobulinemia model. J Clin Invest. 2014;124:4067-81. (Stim)
  26. 26. Bergen HR 3rd, Losman MJ, O'Connor T, Zacharias W, Larson JE, Accavitti MA, et al. Specificity of monoclonal anti-Z-DNA antibodies from unimmunized MRL/Mp-lpr/lpr mice. J Immunol. 1987;139:743-8. (Purification)
See All References

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  1. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  2. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat F(ab')<sub>2</sub> Anti-Mouse IgG(H+L), Human ads-AF488 (SB Cat. No. 1032-30).
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  3. Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) and Rabbit IgG-UNLB (SB Cat. No. 0111-01; left) followed by Donkey F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-AF488 (SB Cat. No. 6442-30) and DAPI.
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  4. HCC827 cells were stained with anti-E-cadherin followed by a secondary antibody and mounted with DAPI Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-20).<br/>Image from Narita M, Shimura E, Nagasawa A, Aiuchi T, Suda Y, Hamada Y, et al. Chronic treatment of non-small-cell lung cancer cells with gefitinib leads to an epigenetic loss of epithelial properties associated with reductions in microRNA-155 and -200c. PLoS One. 2017;12(2):e0172115. Figure 4(e)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  5. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Donkey Anti-Mouse IgG(H+L), Multi-Species SP ads-AF555 (SB Cat. No. 6415-32) and DAPI.
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  6. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  7. FLISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM, Human ads-AF555 (SB Cat. No. 1020-32).
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