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  • Goat F(ab')2 Anti-Mouse IgG(H+L), Human ads-FITC

Goat F(ab')2 Anti-Mouse IgG(H+L), Human ads-FITC

Cat. No.:
1032-02
Goat F(ab')2 Anti-Mouse IgG(H+L)-FITC antibody with minimal reactivity to human proteins for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blot assays.
$94.00
Size Price (USD) Quantity
0.5 mg $94.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat F(ab')2 IgG
Isotype Control Goat F(ab')2 IgG-FITC
Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA
Source Pepsin digest of Goat Anti-Mouse IgG(H+L), Human ads (SB Cat. No. 1031)
Cross Adsorption Human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Quality tested applications for relevant formats include -
ELISA 1,2,24,25
FLISA
Flow Cytometry 4-20

Other referenced applications for relevant formats include -
ELISpot 3
Immunohistochemistry-Frozen Sections 21,26,27
Immunohistochemistry-Paraffin Sections 21
Immunocytochemistry 13,17,22
Western Blot 23,28
RRID Number AB_2794319

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Mansfield LS, Bell JA, Wilson DL, Murphy AJ, Elsheikha HM, Rathinam VA, et al. C57BL/6 and congenic interleukin-10-deficient mice can serve as models of Campylobacter jejuni colonization and enteritis. Infect Immun. 2007;75:1099-115. (ELISA)
  2. 2. Zhang H, Jia C, Xi H, Li S, Yang L, Wang Y. Specific inhibition of Candida albicans growth in vitro by antibodies from experimental Candida keratitis mice. Exp Eye Res. 2011;93:50-8. (ELISA)
  3. 3. Shaw CA, Galarneau J, Bowenkamp KE, Swanson KA, Palmer GA, Palladino G, et al. The role of non-viral antigens in the cotton rat model of respiratory syncytial virus vaccine-enhanced disease. Vaccine. 2013;31:306-12. (ELISPOT)
  4. 4. Xia Y, Ross GD. Generation of recombinant fragments of CD11b expressing the functional β-glucan-binding lectin site of CR3 (CD11b/CD18). J Immunol. 1999;162:7285-93. (FC)
  5. 5. Force WR, Glass AA, Benedict CA, Cheung TC, Lama J, Ware CF. Discrete signaling regions in the lymphotoxin-β receptor for tumor necrosis factor receptor-associated factor binding, subcellular localization, and activation of cell death and NF-κB pathways. J Biol Chem. 2000;275:11121-9. (FC)
  6. 6. Céfai D, Favre L, Wattendorf E, Marti A, Jaggi R, Gimmi CD. Role of Fas ligand expression in promoting escape from immune rejection in a spontaneous tumor model. Int J Cancer. 2001;91:529-37. (FC)
  7. 7. Samten B, Wizel B, Shams H, Weis SE, Klucar P, Wu S, et al. CD40 ligand trimer enhances the response of CD8+ T cells to Mycobacterium tuberculosis. J Immunol. 2003;170:3180-6. (FC)
  8. 8. Ritelli M, Amadori M, Tagliabue S, Pacciarini ML. Use of a macrophage cell line for rapid detection of Mycobacterium bovis in diagnostic samples. Vet Microbiol. 2003;94:105-20. (FC)
  9. 9. McNees AL, Mahr JA, Ornelles D, Gooding LR. Postinternalization inhibition of adenovirus gene expression and infectious virus production in human T-cell lines. J Virol. 2004;78:6955-66. (FC)
  10. 10. Mongini PK, Inman JK, Han H, Kalled SL, Fattah RJ, McCormick S. Innate immunity and human B cell clonal expansion: effects on the recirculating B2 subpopulation. J Immunol. 2005;175:6143-54. (FC)
  11. 11. Kawabata A, Momoi Y, Inoue-Murayama M, Iwasaki T. Canine mdr1 gene mutation in Japan. J Vet Med Sci. 2005;67:1103-7. (FC)
  12. 12. Clancy RM, Neufing PJ, Zheng P, O'Mahony M, Nimmerjahn F, Gordon TP, et al. Impaired clearance of apoptotic cardiocytes is linked to anti-SSA/Ro and -SSB/La antibodies in the pathogenesis of congenital heart block. J Clin Invest. 2006;116:2413-22. (FC)
  13. 13. Shao DD, Suresh R, Vakil V, Gomer RH, Pilling D. Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation. J Leukoc Biol. 2008;83:1323-33. (FC, ICC)
  14. 14. Garnett CT, Talekar G, Mahr JA, Huang W, Zhang Y, Ornelles DA, et al. Latent species C adenoviruses in human tonsil tissues. J Virol. 2009;83:2417-28. (FC)
  15. 15. Drewniak A, van Raam BJ, Geissler J, Tool AT, Mook OR, van den Berg TK, et al. Changes in gene expression of granulocytes during in vivo granulocyte colony-stimulating factor/dexamethasone mobilization for transfusion purposes. Blood. 2009;113:5979-98. (FC)
  16. 16. Miller LA, Gerriets JE, Tyler NK, Abel K, Schelegle ES, Plopper CG, et al. Ozone and allergen exposure during postnatal development alters the frequency and airway distribution of CD25+ cells in infant rhesus monkeys. Toxicol Appl Pharmacol. 2009;236:39-48. (FC)
  17. 17. Montalvão F, Almeida GM, Silva EM, Borges VM, Vasconcellos R, Takiya CM, et al. Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-α secretion and reduce parasite replication in macrophages. Eur J Immunol. 2010;40:417-25. (FC, ICC)
  18. 18. Chmielewski M, Hombach AA, Abken H. CD28 cosignalling does not affect the activation threshold in a chimeric antigen receptor-redirected T-cell attack. Gene Ther. 2011;18:62-72. (FC)
  19. 19. Fournier P, Aigner M, Schirrmacher V. Targeting of IL-2 and GM-CSF immunocytokines to a tumor vaccine leads to increased anti-tumor activity. Int J Oncol. 2011;38:1719-29. (FC)
  20. 20. Guzman R, Valente EG, Pretorius J, Pacheco E, Qi M, Bennett BD, et al. Expression of ORAI1, a plasma membrane resident subunit of the CRAC channel, in rodent and non-rodent species. J Histochem Cytochem. 2014;62:864-78. (FC)
  21. 21. Dasso JF, Obiakor H, Bach H, Anderson AO, Mage RG. A morphological and immunohistological study of the human and rabbit appendix for comparison with the avian bursa. Dev Comp Immunol. 2000;24:797-814. (IHC-FS, IHC-PS)
  22. 22. Crawford JR, Pilling D, Gomer RH. FcγRI mediates serum amyloid P inhibition of fibrocyte differentiation. J Leukoc Biol. 2012;92:699-711. (ICC)
  23. 23. Tosi MF, Zakem H. Surface expression of Fcγ receptor III (CD16) on chemoattractant-stimulated neutrophils is determined by both surface shedding and translocation from intracellular storage compartments. J Clin Invest. 1992;90:462-70. (WB)
  24. 24. Rifkin IR, Leadbetter EA, Beaudette BC, Kiani C, Monestier M, Shlomchik MJ, et al. Immune complexes present in the sera of autoimmune mice activate rheumatoid factor B cells. J Immunol. 2000;165:1626-33. (ELISA)
  25. 25. Singh N, Johnstone DB, Martin KA, Tempera I, Kaplan MJ, Denny MF. Alterations in nuclear structure promote lupus autoimmunity in a mouse model. Dis Model Mech. 2016;9:885-97. (ELISA)
  26. 26. Hande S, Notidis E, Manser T. Bcl-2 obstructs negative selection of autoreactive, hypermutated antibody V regions during memory B cell development. Immunity. 1998;8:189-98. (IHC-FS)
  27. 27. Notidis E, Hande S, Manser T. Enforced expression of Bcl-2 selectively perturbs negative selection of dual reactive antibodies. Dev Immunol. 2001;8:223-34. (IHC-FS)
  28. 28. Szijártó V, Guachalla LM, Visram ZC, Hartl K, Varga C, Mirkina I, et al. Bactericidal monoclonal antibodies specific to the lipopolysaccharide O antigen from multidrug-resistant Escherichia coli clone ST131-O25b:H4 elicit protection in mice. Antimicrob Agents Chemother. 2015;59:3109-16. (WB)
  29. 29. Ohnaga T, Shimada Y, Takata K, Obata T, Okumura T, Nagata T, et al. Capture of esophageal and breast cancer cells with polymeric microfluidic devices for CTC isolation. Mol Clin Oncol. 2016;4:599-602.
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