Goat F(ab')2 Anti-Human Kappa, Mouse ads-PE
Cat. No.:
2063-09
Goat F(ab')2 Anti-Human Kappa-PE antibody with minimal reactivity to mouse proteins for use in flow cytometry assays.
$402.00
![CD19+ human B-lymphocytes were stained with Goat F(ab')<sub>2</sub> Anti-Human Kappa, Mouse ads-PE (SB Cat. 2063-09) and Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-FITC (SB Cat. No. 2073-02).](https://www.southernbiotech.com/media/catalog/product/cache/7149a3d30fe6da616557c86e375dcb18/6/b/6badc9290b1088015a66c2b0d50482e72ad51e20cfbf256198f8366047bd37f6.jpeg)
![CD19+ human B-lymphocytes were stained with Goat F(ab')<sub>2</sub> Anti-Human Kappa, Mouse ads-PE (SB Cat. 2063-09) and Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-FITC (SB Cat. No. 2073-02).](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/6/b/6badc9290b1088015a66c2b0d50482e72ad51e20cfbf256198f8366047bd37f6.jpeg)
![FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Kappa, Mouse ads-PE (SB Cat. No. 2063-09).](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/5/d/5d76c8b0068f5c362791cf42bd1b6e9ab87c49e49daa5a5c5a5ae15dcfc22d00.jpeg)
Isotype | Goat F(ab')2 IgG |
---|---|
Isotype Control | Goat F(ab')2 IgG-PE |
Specificity | Reacts with human κ light chains |
Source | Pepsin digest of Goat Anti-Human Kappa, Mouse ads (SB Cat. No. 2061) |
Cross Adsorption | Human λ light chains; mouse immunoglobulins and pooled sera; may react with κ light chains from other species |
Conjugate | PE (R-phycoerythrin) |
Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
Clonality | Polyclonal |
Concentration | 0.25 mg/mL |
Volume | 1.0 mL |
Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
Applications |
Quality tested applications for relevant formats include - ELISA 3 FLISA Flow Cytometry 1,2 Other referenced applications for relevant formats include - ELISpot 3 Stimulation 3,4 |
RRID Number | AB_2795748 |
Gene ID |
3514 (Human) |
Gene ID Symbol |
IGKC (Human) |
Gene ID Aliases | HCAK1D; Km; IGKC |
UniProt ID |
P01834 (Human) |
UniProt Name |
IGKC_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Akamatsu Y, Pakabunto K, Xu Z, Zhang Y, Tsurushita N. Whole IgG surface display on mammalian cells: Application to isolation of neutralizing chicken monoclonal anti-IL-12 antibodies. J Immunol Methods. 2007;327:40-52. (FC)
- 2. Wilson TJ, Fuchs A, Colonna M. Cutting edge: human FcRL4 and FcRL5 are receptors for IgA and IgG. J Immunol. 2012;188:4741-5. (FC)
- 3. Velez M, Kane M, Liu SM, Gauld SB, Cambier JC, Torres RM, et al. Ig allotypic inclusion does not prevent B cell development or response. J Immunol. 2007;179:1049-57. (ELISA, ELISPOT, Stim)
- 4. Hoogeboom R, van Kessel KP, Hochstenbach F, Wormhoudt TA, Reinten RJ, Wagner K, et al. A mutated B cell chronic lymphocytic leukemia subset that recognizes and responds to fungi. J Exp Med. 2013;210:59-70. (Stim)
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