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  • Goat F(ab')2 Anti-Human Kappa, Mouse ads-FITC

Goat F(ab')2 Anti-Human Kappa, Mouse ads-FITC

Cat. No.:
2063-02
Goat F(ab')2 Anti-Human Kappa-FITC antibody with minimal reactivity to mouse proteins for use in flow cytometry assays.
$286.00
Size Price (USD) Quantity
0.5 mg $286.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat F(ab')2 IgG
Isotype Control Goat F(ab')2 IgG-FITC
Specificity Reacts with human κ light chains
Source Pepsin digest of Goat Anti-Human Kappa, Mouse ads (SB Cat. No. 2061)
Cross Adsorption Human λ light chains; mouse immunoglobulins and pooled sera; may react with κ light chains from other species
Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Quality tested applications for relevant formats include -
ELISA 3
FLISA
Flow Cytometry 1,2

Other referenced applications for relevant formats include -
ELISpot 3
Stimulation 3,4
RRID Number AB_2795745
Gene ID 3514 (Human)
Gene ID Symbol IGKC (Human)
Gene ID Aliases HCAK1D; Km; IGKC
UniProt ID P01834 (Human
UniProt Name IGKC_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Akamatsu Y, Pakabunto K, Xu Z, Zhang Y, Tsurushita N. Whole IgG surface display on mammalian cells: Application to isolation of neutralizing chicken monoclonal anti-IL-12 antibodies. J Immunol Methods. 2007;327:40-52. (FC)
  2. 2. Wilson TJ, Fuchs A, Colonna M. Cutting edge: human FcRL4 and FcRL5 are receptors for IgA and IgG. J Immunol. 2012;188:4741-5. (FC)
  3. 3. Velez M, Kane M, Liu SM, Gauld SB, Cambier JC, Torres RM, et al. Ig allotypic inclusion does not prevent B cell development or response. J Immunol. 2007;179:1049-57. (ELISA, ELISPOT, Stim)
  4. 4. Hoogeboom R, van Kessel KP, Hochstenbach F, Wormhoudt TA, Reinten RJ, Wagner K, et al. A mutated B cell chronic lymphocytic leukemia subset that recognizes and responds to fungi. J Exp Med. 2013;210:59-70. (Stim)
See All References

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  2. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-FITC (SB Cat. No. 2070-02).
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  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  4. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa, Mouse ads-FITC (SB Cat. No. 2061-02).
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  5. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Kappa-FITC (SB Cat. No. 2062-02).
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  6. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Mouse Anti-Human Kappa-FITC (SB Cat. No. 9230-02).
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  7. CD19+ human B-lymphocytes were stained with Goat Anti-Human Kappa-FITC (SB Cat. 2060-02) and Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-PE (SB Cat. No. 2073-09).
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  8. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda, Mouse ads-FITC (SB Cat. No. 2071-02).
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  9. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Lambda-FITC (SB Cat. No. 2072-02).
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  10. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-FITC (SB Cat. No. 2073-02).
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  11. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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