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  • Goat F(ab')2 Anti-Human Kappa, Mouse ads-BIOT

Goat F(ab')2 Anti-Human Kappa, Mouse ads-BIOT

Cat. No.:
2063-08
Goat F(ab')2 Anti-Human Kappa-Biotin antibody with minimal reactivity to mouse proteins for use in ELISA, flow cytometry, ELISpot, and stimulation assays.
$286.00
Size Price (USD) Quantity
0.5 mg $286.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat F(ab')2 IgG
Isotype Control Goat F(ab')2 IgG-BIOT
Specificity Reacts with human κ light chains
Source Pepsin digest of Goat Anti-Human Kappa, Mouse ads (SB Cat. No. 2061)
Cross Adsorption Human λ light chains; mouse immunoglobulins and pooled sera; may react with κ light chains from other species
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 3
FLISA
Flow Cytometry 1,2

Other referenced applications for relevant formats include -
ELISpot 3
Stimulation 3,4
RRID Number AB_2795747
Gene ID 3514 (Human)
Gene ID Symbol IGKC (Human)
Gene ID Aliases HCAK1D; Km; IGKC
UniProt ID P01834 (Human
UniProt Name IGKC_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Akamatsu Y, Pakabunto K, Xu Z, Zhang Y, Tsurushita N. Whole IgG surface display on mammalian cells: Application to isolation of neutralizing chicken monoclonal anti-IL-12 antibodies. J Immunol Methods. 2007;327:40-52. (FC)
  2. 2. Wilson TJ, Fuchs A, Colonna M. Cutting edge: human FcRL4 and FcRL5 are receptors for IgA and IgG. J Immunol. 2012;188:4741-5. (FC)
  3. 3. Velez M, Kane M, Liu SM, Gauld SB, Cambier JC, Torres RM, et al. Ig allotypic inclusion does not prevent B cell development or response. J Immunol. 2007;179:1049-57. (ELISA, ELISPOT, Stim)
  4. 4. Hoogeboom R, van Kessel KP, Hochstenbach F, Wormhoudt TA, Reinten RJ, Wagner K, et al. A mutated B cell chronic lymphocytic leukemia subset that recognizes and responds to fungi. J Exp Med. 2013;210:59-70. (Stim)
See All References

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  1. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat F(ab')<sub>2</sub> IgG-BIOT (SB Cat No. 0110-08; gray) and Goat F(ab')<sub>2</sub> Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1072-08) then Streptavidin-FITC (SB Cat. No. 7100-02).
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  2. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-BIOT (SB Cat. No. 2070-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  3. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
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  4. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Kappa-UNLB (SB Cat. No. 0151K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  6. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa, Mouse ads-BIOT (SB Cat. No. 2061-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Human Kappa, Mouse ads-BIOT
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  7. FLISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>2b</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Mouse Anti-Biotin-FITC (SB Cat. No. 6404-02).
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  8. ELISA plate was coated with serially diluted Human IgA Kappa-UNLB (SB Cat. No. 0155K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  9. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Kappa-BIOT (SB Cat. No. 2062-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  10. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  11. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa-BIOT (SB Cat. No. 2060-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  12. CD19+ human B-lymphocytes were stained with Goat Anti-Human Lambda, Mouse ads-BIOT (SB Cat. 2071-08) and Goat F(ab')<sub>2</sub> Anti-Human Kappa, Mouse ads-PE (SB Cat. No. 2063-09) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
    Goat Anti-Human Lambda, Mouse ads-BIOT
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  13. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Lambda-BIOT (SB Cat. No. 2072-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  14. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-AP (SB Cat. No. 7105-04).
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  15. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-BIOT (SB Cat. No. 2073-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  16. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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