My Cart
$0.00
0

Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

  • Home
  • Goat F(ab')2 Anti-Human IgG-UNLB

Goat F(ab')2 Anti-Human IgG-UNLB

Cat. No.:
2042-01
Purified Goat F(ab')2 Anti-Human IgG antibody for use in ELISA, flow cytometry, immunohistochemistry, western blot, ELISpot, separation, and stimulation assays.
$83.00
Size Price (USD) Quantity
0.5 mg $83.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat F(ab')2 IgG
Isotype Control Goat F(ab')2 IgG-UNLB
Specificity Reacts with the heavy chain of human IgG
Source Pepsin digest of Goat Anti-Human IgG (SB Cat. No. 2040)
Cross Adsorption Human IgM and IgA; may react with IgG from other species
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1-4,6
FLISA
Flow Cytometry 12-18

Other referenced applications for relevant formats include -
ELISpot 5
Immunohistochemistry-Frozen Sections 7-9
Western Blot 10,11
Separation 19
Stimulation 20,21
RRID Number AB_2795657

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
Email technical support
or call 800.722.2255

Other Conjugates and Formulations

Citations

  1. 1. Leyendeckers H, Odendahl M, Löhndorf A, Irsch J, Spangfort M, Miltenyi S, et al. Correlation analysis between frequencies of circulating antigen-specific IgG-bearing memory B cells and serum titers of antigen-specific IgG. Eur J Immunol. 1999;29:1406-17. (ELISA)
  2. 2. Leyendeckers H, Tasanen K, Bruckner-Tuderman L, Zillikens D, Sitaru C, Schmitz J, et al. Memory B cells specific for the NC16A domain of the 180 kDa bullous pemphigoid autoantigen can be detected in peripheral blood of bullous pemphigoid patients and induced in vitro to synthesize autoantibodies. J Invest Dermatol. 2003;120:372-8. (ELISA)
  3. 3. Matousovic K, Novak J, Yanagihara T, Tomana M, Moldoveanu Z, Kulhavy R, et al. IgA-containing immune complexes in the urine of IgA nephropathy patients. Nephrol Dial Transplant. 2006;21:2478-84. (ELISA)
  4. 4. de Groot-Mijnes JD, de Visser L, Rothova A, Schuller M, van Loon AM, Weersink AJ. Rubella virus is associated with fuchs heterochromic iridocyclitis. Am J Ophthalmol. 2006;141:212-4. (ELISA)
  5. 5. Sira MM, Yoshida T, Takeuchi M, Kashiwayama Y, Futatani T, Kanegane H, et al. A novel immunoregulatory protein in human colostrum, syntenin-1, for promoting the development of IgA-producing cells from cord blood B cells. Int Immunol. 2009;21:1013-23. (ELISPOT)
  6. 6. White CA, Pone EJ, Lam T, Tat C, Hayama KL, Li G, et al. Histone deacetylase inhibitors upregulate B cell microRNAs that silence AID and Blimp-1 expression for epigenetic modulation of antibody and autoantibody responses. J Immunol. 2014;193:5933-50. (ELISA)
  7. 7. Xu W, He B, Chiu A, Chadburn A, Shan M, Buldys M, et al. Epithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLPI. Nat Immunol. 2007;8:294-303. (IHC-FS)
  8. 8. He B, Xu W, Santini PA, Polydorides AD, Chiu A, Estrella J, et al. Intestinal bacteria trigger T cell-independent immunoglobulin A2 class switching by inducing epithelial-cell secretion of the cytokine APRIL. Immunity. 2007;26:812-26. (IHC-FS)
  9. 9. Xu W, Santini PA, Matthews AJ, Chiu A, Plebani A, He B, et al. Viral double-stranded RNA triggers Ig class switching by activating upper respiratory mucosa B cells through an innate TLR3 pathway involving BAFF. J Immunol. 2008;181:276-87. (IHC-FS)
  10. 10. Moldoveanu Z, Huang W, Kulhavy R, Pate MS, Mestecky J. Human male genital tract secretions: both mucosal and systemic immune compartments contribute to the humoral immunity. J Immunol. 2005;175:4127-36. (WB)
  11. 11. Yamasaki Y, Narain S, Hernandez L, Barker T, Ikeda K, Segal MS, et al. Autoantibodies against the replication protein A complex in systemic lupus erythematosus and other autoimmune diseases. Arthritis Res Ther. 2006;8:R111. (WB)
  12. 12. Scholz C, Patton KT, Anderson DE, Freeman GJ, Hafler DA. Expansion of autoreactive T cells in multiple sclerosis is independent of exogenous B7 costimulation. J Immunol. 1998;160:1532-8. (FC)
  13. 13. Janoff EN, Fasching C, Orenstein JM, Rubins JB, Opstad NL, Dalmasso AP. Killing of Streptococcus pneumoniae by capsular polysaccharide-specific polymeric IgA, complement, and phagocytes. J Clin Invest. 1999;104:1139-47. (FC)
  14. 14. van der Pol W, Vidarsson G, Vilé HA, van de Winkel JG, Rodriguez ME. Pneumococcal capsular polysaccharide-specific IgA triggers efficient neutrophil effector functions via FcαRI (CD89). J Infect Dis. 2000;182:1139-45. (FC)
  15. 15. Rodriguez ME, Hellwig SM, Hozbor DF, Leusen J, van der Pol W, van de Winkel JG. Fc receptor-mediated immunity against Bordetella pertussis. J Immunol. 2001;167:6545-51. (FC)
  16. 16. He B, Santamaria R, Xu W, Cols M, Chen K, Puga I, et al. The transmembrane activator TACI triggers immunoglobulin class switching by activating B cells through the adaptor MyD88. Nat Immunol. 2010;11:836-45. (FC)
  17. 17. Lin F, Elliott R, Colombero A, Gaida K, Kelley L, Moksa A, et al. Generation and characterization of fully human monoclonal antibodies against human Orai1 for autoimmune disease. J Pharmacol Exp Ther. 2013;345:225-38. (FC)
  18. 18. Wagner K, Kwakkenbos MJ, Claassen YB, Maijoor K, Böhne M, van der Sluijs KF, et al. Bispecific antibody generated with sortase and click chemistry has broad antiinfluenza virus activity. Proc Natl Acad Sci USA. 2014;111:16820-5. (FC)
  19. 19. Quan J, Lakhanpal A, Reddy MM, Zaman S, Li Q, German DC, et al. Discovery of biomarkers for systemic lupus erythematosus using a library of synthetic autoantigen surrogates. J Immunol Methods. 2014;402:23-34. (Sep)
  20. 20. Suljagic M, Laurenti L, Tarnani M, Alam M, Malek SN, Efremov DG. Reduced expression of the tumor suppressor PHLPP1 enhances the antiapoptotic B-cell receptor signal in chronic lymphocytic leukemia B-cells. Leukemia. 2010;24:2063-71. (Stim)
  21. 21. Cheng S, Coffey G, Zhang XH, Shaknovich R, Song Z, Lu P, et al. SYK inhibition and response prediction in diffuse large B-cell lymphoma. Blood. 2011;118:6342-52. (Stim)
See All References

Related Products

Related Products

Related Products
Check items to add to the cart or
  1. ELISA plate was coated with serially diluted Goat F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0110-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) and Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Goat F(ab')2 IgG-UNLB
    0110-01
    $191.00
  2. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG-UNLB (SB Cat. No. 2040-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
    Goat Anti-Human IgG-UNLB
    2040-01
    $77.00
  3. ELISA plate was coated with purified human IgG, IgM, and IgA, and mouse IgG, IgM, and IgA (mouse Ig). Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human IgG, Mouse ads-UNLB (SB Cat. No. 2043-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
    Goat F(ab')2 Anti-Human IgG, Mouse ads-UNLB
    2043-01
    $121.00
  4. Human IgG-UNLB (SB Cat. No. 0150-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9040-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
    Human IgG-UNLB
    0150-01
    $55.00
  5. FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF488 (SB Cat. No. 6425-30).
    Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF488
    6425-30
    $259.00
  6. Goat F(ab')2 Anti-Human IgA-UNLB
    Goat F(ab')2 Anti-Human IgA-UNLB
    2052-01
    $88.00
  7. ELISA plate was coated with purified human IgM, IgG, and IgA and mouse, bovine, and horse serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Human IgM, Mouse/Bovine/Horse SP ads-UNLB (SB Cat. No. 2023-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
    Goat Anti-Human IgM, Mouse/Bovine/Horse SP ads-UNLB
    2023-01
    $105.00
  8. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Fluoromount-G®
    0100-01
    $45.00
  9. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Kappa-UNLB (SB Cat. No. 0151K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Human IgG1 Kappa-UNLB
    0151K-01
    $110.00
  10. Human IgG-UNLB (SB Cat. No. 0150-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9040-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
    Mouse Anti-Human IgG Fc-UNLB (JDC-10)
    9040-01
    $143.00
  11. Ramos cell lysates treated with HM71224 and stimulated with Goat F(ab')<sub>2</sub> Anti-Human IgM-UNLB (SB Cat. No. 2022-01) were resolved by electrophoresis, transferred to PVDF membrane, and probed with anti-phospho-PLCγ2Y1217 followed by an HRP conjugated secondary antibody and chemiluminescent detection.<br/>Image from Kim Y, Park KT, Jang SY, Lee KH, Byun J, Suh KH, et al. HM71224, a selective Bruton's tyrosine kinase inhibitor, attenuates the development of murine lupus. Arthritis Res Ther. 2017;19:211. Figure 1(a)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Goat F(ab')2 Anti-Human IgM-UNLB
    2022-01
    $84.00
  12. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit F(ab')<sub>2</sub> Anti-Goat IgG(H+L), Human SP ads-HRP (SB Cat. No. 6026-05).
    Rabbit F(ab')2 Anti-Goat IgG(H+L), Human SP ads-HRP
    6026-05
    $132.00
  13. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Kappa-UNLB (SB Cat. No. 2062-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/ Mouse SP ads-HRP (SB Cat. No. 6300-05).
    Goat F(ab')2 Anti-Human Kappa-UNLB
    2062-01
    $132.00
  14. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Lambda-UNLB (SB Cat. No. 0151L-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Human IgG1 Lambda-UNLB
    0151L-01
    $110.00
  15. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit Anti-Goat IgG(H+L), Human SP ads-AP (SB Cat. No. 6164-04).
    Rabbit Anti-Goat IgG(H+L), Human SP ads-AP
    6164-04
    $132.00
  16. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT (SB Cat. No. 6300-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
    Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT
    6300-08
    $105.00
  17. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9042-01) followed by Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 1038-08) and Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Human IgG Fc-UNLB (H2)
    9042-01
    $143.00
  18. ELISA plate was coated with serially diluted Human IgG Fc-UNLB (SB Cat. No. 0160-01). Immunoglobulin was detected with Goat Anti-Human IgG Fc-BIOT (SB Cat. No. 2048-08), Goat Anti-Human Ig Fab-BIOT (SB Cat. No. 2085-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT and Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9130-08 and Cat. No. 9140-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Human IgG Fc-UNLB
    0160-01
    $215.00
  19. Goat F(ab')2 Anti-Human Lambda-UNLB
    Goat F(ab')2 Anti-Human Lambda-UNLB
    2072-01
    $132.00
  20. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
    Normal Goat Serum
    0060-01
    $44.00
  21. Human peripheral blood lymphocytes were stained with Goat F(ab')<sub>2</sub> Anti-Human IgD-UNLB (SB Cat. 2032-01) and Mouse Anti-Human CD19-PE (SB Cat. No. 9340-09) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-FITC (SB Cat. No. 6300-02).
    Goat F(ab')2 Anti-Human IgD-UNLB
    2032-01
    $112.00