My Cart
$0.00
0

Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

  • Home
  • Goat F(ab')2 Anti-Human IgA-BIOT

Goat F(ab')2 Anti-Human IgA-BIOT

Cat. No.:
2052-08
Goat F(ab')2 Anti-Human IgA-Biotin antibody for use in ELISA, flow cytometry, immunohistochemistry, and ELISpot assays.
$105.00
Size Price (USD) Quantity
0.5 mg $105.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat F(ab')2 IgG
Isotype Control Goat F(ab')2 IgG-BIOT
Specificity Reacts with the heavy chain of human IgA
Source Pepsin digest of Goat Anti-Human IgA (SB Cat. No. 2050)
Cross Adsorption Human IgG and IgM; may react with IgA from other species
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1-4,6
FLISA
Flow Cytometry 9-16

Other referenced applications for relevant formats include -
ELISpot 5
Immunohistochemistry-Frozen Sections 7,8
Stimulation 17
RRID Number AB_2687520

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
Email technical support
or call 800.722.2255

Other Conjugates and Formulations

Citations

  1. 1. Guttormsen H, Baker CJ, Edwards MS, Paoletti LC, Kasper DL. Quantitative determination of antibodies to type III group B streptococcal polysaccharide. J Infect Dis. 1996;173:142-50. (ELISA)
  2. 2. Matousovic K, Novak J, Yanagihara T, Tomana M, Moldoveanu Z, Kulhavy R, et al. IgA-containing immune complexes in the urine of IgA nephropathy patients. Nephrol Dial Transplant. 2006;21:2478-84. (ELISA)
  3. 3. Huggins J, Pellegrin T, Felgar RE, Wei C, Brown M, Zheng B, et al. CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells. Blood. 2007;109:1611-9. (ELISA)
  4. 4. Berthelot L, Papista C, Maciel TT, Biarnes-Pelicot M, Tissandie E, Wang PH, et al. Transglutaminase is essential for IgA nephropathy development acting through IgA receptors. J Exp Med. 2012;209:793-806. (ELISA)
  5. 5. Sira MM, Yoshida T, Takeuchi M, Kashiwayama Y, Futatani T, Kanegane H, et al. A novel immunoregulatory protein in human colostrum, syntenin-1, for promoting the development of IgA-producing cells from cord blood B cells. Int Immunol. 2009;21:1013-23. (ELISpot)
  6. 6. White CA, Pone EJ, Lam T, Tat C, Hayama KL, Li G, et al. Histone deacetylase inhibitors upregulate B cell microRNAs that silence AID and Blimp-1 expression for epigenetic modulation of antibody and autoantibody responses. J Immunol. 2014;193:5933-50. (ELISA)
  7. 7. Boumediene A, Oblet C, Oruc Z, Duchez S, Morelle W, Huynh A, et al. Gammopathy with IgA mesangial deposition provides a monoclonal model of IgA nephritogenicity and offers new insights into its molecular mechanisms. Nephrol Dial Transplant. 2011;26:3930-7. (IHC-FS)
  8. 8. Burgener A, Tjernlund A, Kaldensjo T, Abou M, McCorrister S, Westmacott GR, et al. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. J Virol. 2013;87:5141-50. (IHC-FS)
  9. 9. Grossetête B, Launay P, Lehuen A, Jungers P, Bach J, Monteiro RC. Down-regulation of Fcα receptors on blood cells of IgA nephropathy patients: evidence for a negative regulatory role of serum IgA. Kidney Int. 1998;53:1321-35. (FC)
  10. 10. Morton HC, van Zandbergen G, van Kooten C, Howard CJ, van de Winkel JG, Brandtzaeg P. Immunoglobulin-binding sites of human FcαRI (CD89) and bovine Fcγ2R are located in their membrane-distal extracellular domains. J Exp Med. 1999;189:1715-22. (FC)
  11. 11. Janoff EN, Fasching C, Orenstein JM, Rubins JB, Opstad NL, Dalmasso AP. Killing of Streptococcus pneumoniae by capsular polysaccharide-specific polymeric IgA, complement, and phagocytes. J Clin Invest. 1999;104:1139-47. (FC)
  12. 12. van der Pol W, Vidarsson G, Vilé HA, van de Winkel JG, Rodriguez ME. Pneumococcal capsular polysaccharide-specific IgA triggers efficient neutrophil effector functions via FcαRI (CD89). J Infect Dis. 2000;182:1139-45. (FC)
  13. 13. Rodriguez ME, Hellwig SM, Hozbor DF, Leusen J, van der Pol W, van de Winkel JG. Fc receptor-mediated immunity against Bordetella pertussis. J Immunol. 2001;167:6545-51. (FC)
  14. 14. Honorio-Franca AC, Launay P, Carneiro-Sampaio MM, Monteiro RC. Colostral neutrophils express Fcα receptors (CD89) lacking γ chain association and mediate noninflammatory properties of secretory IgA. J Leukoc Biol. 2001;69:289-96. (FC)
  15. 15. Heystek HC, Moulon C, Woltman AM, Garonne P, van Kooten C. Human immature dendritic cells efficiently bind and take up secretory IgA without the induction of maturation. J Immunol. 2002;168:102-7. (FC)
  16. 16. Mota G, Manciulea M, Cosma E, Popescu I, Hirt M, Jensen-Jarolim E, et al. Human NK cells express Fc receptors for IgA which mediate signal transduction and target cell killing. Eur J Immunol. 2003;33:2197-205. (FC)
  17. 17. Toapanta FR, Bernal PJ, Sztein MB. Diverse phosphorylation patterns of B cell receptor-associated signaling in naïve and memory human B cells revealed by phosphoflow, a powerful technique to study signaling at the single cell level. Front Cell Infect Microbiol. 2012;2:128. (Stim)
See All References

Related Products

Related Products

Related Products
Check items to add to the cart or
  1. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat F(ab')<sub>2</sub> IgG-BIOT (SB Cat No. 0110-08; gray) and Goat F(ab')<sub>2</sub> Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1072-08) then Streptavidin-FITC (SB Cat. No. 7100-02).
    Goat F(ab')2 IgG-BIOT
    0110-08
    $94.00
  2. ELISA plate was coated with serially diluted Human IgA Kappa-UNLB (SB Cat. No. 0155K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Human IgA Kappa-UNLB
    0155K-01
    $110.00
  3. ELISA plate was coated with purified human IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Human IgA-BIOT (SB Cat. No. 2050-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Human IgA-BIOT
    2050-08
    $99.00
  4. ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human IgM-BIOT (SB Cat. No. 2022-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Human IgM-BIOT
    2022-08
    $121.00
  5. ELISA plate was coated with serially diluted Human IgA Lambda-UNLB (SB Cat. No. 0155L-01). Immunoglobulin was detected with Mouse Anti-Human IgG-BIOT (SB Cat. No. 9040-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Human IgA Lambda-UNLB
    0155L-01
    $110.00
  6. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
    Streptavidin-HRP
    7105-05
    $121.00
  7. ELISA plate was coated with purified human IgA<sub>1</sub>, IgA<sub>2</sub>, IgD, IgG, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Human IgA1-BIOT (B3506B4)
    9130-08
    $215.00
  8. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Fluoromount-G®
    0100-01
    $45.00
  9. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-AP (SB Cat. No. 7105-04).
    Streptavidin-AP
    7105-04
    $121.00
  10. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human IgG-BIOT (SB Cat. No. 2042-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Human IgG-BIOT
    2042-08
    $99.00
  11. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45R-BIOT (SB Cat. No. 1665-08) followed by Mouse Anti-Biotin-PE (SB Cat. No. 6404-09).
    Mouse Anti-Biotin-PE (SB58c)
    6404-09
    $182.00
  12. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Kappa-BIOT (SB Cat. No. 2062-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Human Kappa-BIOT
    2062-08
    $149.00
  13. ELISA plate was coated with purified human IgA<sub>1</sub>, IgA<sub>2</sub>, IgD, IgG, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Human IgA2-BIOT (A9604D2)
    9140-08
    $215.00
  14. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Lambda-BIOT (SB Cat. No. 2072-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Human Lambda-BIOT
    2072-08
    $149.00
  15. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
    Normal Goat Serum
    0060-01
    $44.00
  16. ELISA plate was coated with purified human IgD, IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human IgD-BIOT (SB Cat. No. 2032-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Human IgD-BIOT
    2032-08
    $130.00