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  • Goat F(ab')2 Anti-Human IgA-AF488

Goat F(ab')2 Anti-Human IgA-AF488

Cat. No.:
2052-30
Goat F(ab')2 Anti-Human IgA-Alexa Fluor® 488 antibody for use in flow cytometry and immunohistochemistry assays.
$193.00
Size Price (USD) Quantity
0.5 mg $193.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat F(ab')2 IgG
Isotype Control 0110-30
Specificity Reacts with the heavy chain of human IgA
Source Pepsin digest of Goat Anti-Human IgA (SB Cat. No. 2050)
Cross Adsorption Human IgG and IgM; may react with IgA from other species
Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA 1-4,6
FLISA
Flow Cytometry 9-16

Other referenced applications for relevant formats include -
ELISpot 5
Immunohistochemistry-Frozen Sections 7,8
Stimulation 17
RRID Number AB_2895750

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Guttormsen H, Baker CJ, Edwards MS, Paoletti LC, Kasper DL. Quantitative determination of antibodies to type III group B streptococcal polysaccharide. J Infect Dis. 1996;173:142-50. (ELISA)
  2. 2. Matousovic K, Novak J, Yanagihara T, Tomana M, Moldoveanu Z, Kulhavy R, et al. IgA-containing immune complexes in the urine of IgA nephropathy patients. Nephrol Dial Transplant. 2006;21:2478-84. (ELISA)
  3. 3. Huggins J, Pellegrin T, Felgar RE, Wei C, Brown M, Zheng B, et al. CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells. Blood. 2007;109:1611-9. (ELISA)
  4. 4. Berthelot L, Papista C, Maciel TT, Biarnes-Pelicot M, Tissandie E, Wang PH, et al. Transglutaminase is essential for IgA nephropathy development acting through IgA receptors. J Exp Med. 2012;209:793-806. (ELISA)
  5. 5. Sira MM, Yoshida T, Takeuchi M, Kashiwayama Y, Futatani T, Kanegane H, et al. A novel immunoregulatory protein in human colostrum, syntenin-1, for promoting the development of IgA-producing cells from cord blood B cells. Int Immunol. 2009;21:1013-23. (ELISpot)
  6. 6. White CA, Pone EJ, Lam T, Tat C, Hayama KL, Li G, et al. Histone deacetylase inhibitors upregulate B cell microRNAs that silence AID and Blimp-1 expression for epigenetic modulation of antibody and autoantibody responses. J Immunol. 2014;193:5933-50. (ELISA)
  7. 7. Boumediene A, Oblet C, Oruc Z, Duchez S, Morelle W, Huynh A, et al. Gammopathy with IgA mesangial deposition provides a monoclonal model of IgA nephritogenicity and offers new insights into its molecular mechanisms. Nephrol Dial Transplant. 2011;26:3930-7. (IHC-FS)
  8. 8. Burgener A, Tjernlund A, Kaldensjo T, Abou M, McCorrister S, Westmacott GR, et al. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. J Virol. 2013;87:5141-50. (IHC-FS)
  9. 9. Grossetête B, Launay P, Lehuen A, Jungers P, Bach J, Monteiro RC. Down-regulation of Fcα receptors on blood cells of IgA nephropathy patients: evidence for a negative regulatory role of serum IgA. Kidney Int. 1998;53:1321-35. (FC)
  10. 10. Morton HC, van Zandbergen G, van Kooten C, Howard CJ, van de Winkel JG, Brandtzaeg P. Immunoglobulin-binding sites of human FcαRI (CD89) and bovine Fcγ2R are located in their membrane-distal extracellular domains. J Exp Med. 1999;189:1715-22. (FC)
  11. 11. Janoff EN, Fasching C, Orenstein JM, Rubins JB, Opstad NL, Dalmasso AP. Killing of Streptococcus pneumoniae by capsular polysaccharide-specific polymeric IgA, complement, and phagocytes. J Clin Invest. 1999;104:1139-47. (FC)
  12. 12. van der Pol W, Vidarsson G, Vilé HA, van de Winkel JG, Rodriguez ME. Pneumococcal capsular polysaccharide-specific IgA triggers efficient neutrophil effector functions via FcαRI (CD89). J Infect Dis. 2000;182:1139-45. (FC)
  13. 13. Rodriguez ME, Hellwig SM, Hozbor DF, Leusen J, van der Pol W, van de Winkel JG. Fc receptor-mediated immunity against Bordetella pertussis. J Immunol. 2001;167:6545-51. (FC)
  14. 14. Honorio-Franca AC, Launay P, Carneiro-Sampaio MM, Monteiro RC. Colostral neutrophils express Fcα receptors (CD89) lacking γ chain association and mediate noninflammatory properties of secretory IgA. J Leukoc Biol. 2001;69:289-96. (FC)
  15. 15. Heystek HC, Moulon C, Woltman AM, Garonne P, van Kooten C. Human immature dendritic cells efficiently bind and take up secretory IgA without the induction of maturation. J Immunol. 2002;168:102-7. (FC)
  16. 16. Mota G, Manciulea M, Cosma E, Popescu I, Hirt M, Jensen-Jarolim E, et al. Human NK cells express Fc receptors for IgA which mediate signal transduction and target cell killing. Eur J Immunol. 2003;33:2197-205. (FC)
  17. 17. Toapanta FR, Bernal PJ, Sztein MB. Diverse phosphorylation patterns of B cell receptor-associated signaling in naïve and memory human B cells revealed by phosphoflow, a powerful technique to study signaling at the single cell level. Front Cell Infect Microbiol. 2012;2:128. (Stim)
See All References

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