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  • Goat Anti-Rat IgG(H+L), Mouse/Human ads-UNLB

Goat Anti-Rat IgG(H+L), Mouse/Human ads-UNLB

Cat. No.:
3051-01
Purified Goat Anti-Rat IgG(H+L) antibody with minimal reactivity to mouse and human proteins for use in ELISA and flow cytometry assays.
$121.00
Size Price (USD) Quantity
0.5 mg $121.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Specificity Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Source Pooled antisera from goats hyperimmunized with rat IgG
Cross Adsorption Mouse and human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rat IgG covalently linked to agarose
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA
Flow Cytometry

RRID Number AB_2795839

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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  2. Donkey Anti-Rat IgG(H+L), Mouse SP ads-UNLB
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  3. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  4. Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AF647
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  5. ELISA plate was coated with purified rat IgG and IgM and mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rat Ig, Mouse ads-UNLB (SB Cat. No. 3010-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
    Goat Anti-Rat Ig, Mouse ads-UNLB
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    $149.00
  6. Purified Human Type I Collagen (SB Cat. No. 1200-01S) was resolved by electrophoresis, transferred to PVDF membrane, probed with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01), and visualized using Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6425-05) secondary antibody and chemiluminescent detection.
    Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP
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    $138.00
  7. ELISA plate was coated with purified rat IgG and IgM and human IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Rat IgG(H+L), Human ads-UNLB (SB Cat. No. 6185-01) followed by Rat Anti-Rabbit Ig-UNLB (SB Cat. No. 4065-05).
    Rabbit Anti-Rat IgG(H+L), Human ads-UNLB
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  8. ELISA plate was coated with purified rat IgG and IgM and mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG(H+L), Mouse ads-UNLB (SB Cat. No. 3050-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  9. ELISA plate was coated with serially diluted Rat IgG-UNLB (SB Cat. No. 0108-01). Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) and Mouse Anti-Rat IgM-BIOT (SB Cat. No. 3080-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  10. FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-PE (SB Cat. No. 6300-09).
    Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-PE
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  11. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Rabbit Anti-Rat IgG(H+L)-UNLB (SB Cat. No. 6180-01) followed by Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05).
    Rabbit Anti-Rat IgG(H+L)-UNLB
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  12. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG-UNLB (SB Cat. No. 3030-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
    Goat Anti-Rat IgG-UNLB
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  13. ELISA plate was coated with serially diluted Rat IgM-UNLB (SB Cat. No. 0120-01). Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) and Goat Anti-Rat IgM-BIOT (SB Cat. No. 3020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  14. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey F(ab')<sub>2</sub> Anti-Goat IgG(H+L), Mouse/Rat SP ads-AP (SB Cat. No. 6421-04).
    Donkey F(ab')2 Anti-Goat IgG(H+L), Mouse/Rat SP ads-AP
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  15. Rabbit F(ab')2 Anti-Rat IgG(H+L)-UNLB
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  16. ELISA plate was coated with purified rat IgG and IgM and mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rat IgG(H+L), Mouse ads-UNLB (SB Cat. No. 3052-01) followed by Swine Anti-Goat IgG(H+L), Human/ Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
    Goat F(ab')2 Anti-Rat IgG(H+L), Mouse ads-UNLB
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  17. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Fluoromount-G®
    0100-01
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  18. ELISA plate was coated with purified rat IgG and IgM and mouse serum. Immunoglobulins and serum were detected with serially diluted Mouse Anti-Rat IgG(H+L), Mouse SP ads-UNLB (SB Cat. No. 3053-01) followed by Goat Anti-Mouse IgG(H+L), Rat ads-HRP (SB Cat. No. 1034-05).
    Mouse Anti-Rat IgG(H+L), Mouse SP ads-UNLB
    3053-01
    $99.00
  19. Sheep Anti-Rat IgG(H+L)-UNLB
    Sheep Anti-Rat IgG(H+L)-UNLB
    6900-01
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  20. ELISA plate was coated with purified rat IgG and IgM and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Rabbit F(ab')<sub>2</sub> Anti-Rat IgG(H+L), Human ads-UNLB (SB Cat. No. 6135-01) followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 4050-05).
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  21. FLISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-PE (SB Cat. No. 6157-09).
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  22. ELISA plate was coated with purified rat IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgM-UNLB (SB Cat. No. 3020-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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