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  • Goat Anti-Rat IgG(H+L), Mouse/Human ads-FITC

Goat Anti-Rat IgG(H+L), Mouse/Human ads-FITC

Cat. No.:
3051-02
Goat Anti-Rat IgG(H+L)-FITC antibody with minimal reactivity to mouse and human proteins for use in flow cytometry and other fluorescent assays.
$143.00
Size Price (USD) Quantity
0.5 mg $143.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-FITC
Specificity Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Source Pooled antisera from goats hyperimmunized with rat IgG
Cross Adsorption Mouse and human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rat IgG covalently linked to agarose
Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Quality tested applications for relevant formats include -
ELISA
FLISA
Flow Cytometry

RRID Number AB_2795840

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. Frozen mouse lymph node section was stained with Goat IgG-FITC isotype control (SB Cat. No. 0109-02; left) and Goat Anti-Mouse IgM, Human ads-FITC (SB Cat. No. 1020-02; right) followed by DAPI.
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  2. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Goat Anti-Rat Ig, Mouse ads-FITC (SB Cat. No. 3010-02).
    Goat Anti-Rat Ig, Mouse ads-FITC
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  3. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  5. FLISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Rabbit Anti-Rat IgG(H+L), Human ads-FITC (SB Cat. No. 6185-02).
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  6. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD4-UNLB (SB Cat. 1540-01) followed by Goat Anti-Rat IgG(H+L), Mouse ads-FITC (SB Cat. No. 3050-02).
    Goat Anti-Rat IgG(H+L), Mouse ads-FITC
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  7. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  8. FLISA plate was coated with purified rat IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgM-FITC (SB Cat. No. 3020-02).
    Goat Anti-Rat IgM-FITC
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  9. FLISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Rabbit Anti-Rat IgG(H+L)-FITC (SB Cat. No. 6180-02).
    Rabbit Anti-Rat IgG(H+L)-FITC
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  10. FLISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG-FITC (SB Cat. No. 3030-02).
    Goat Anti-Rat IgG-FITC
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  11. Rabbit F(ab')2 Anti-Rat IgG(H+L)-FITC
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  12. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Goat F(ab')<sub>2</sub> Anti-Rat IgG(H+L), Mouse ads-FITC (SB Cat. No. 3052-02).
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  13. FLISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Rabbit F(ab')<sub>2</sub> Anti-Rat IgG(H+L), Human ads-FITC (SB Cat. No. 6135-02).
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