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  • Goat Anti-Rat IgG(H+L), Mouse ads-TXRD

Goat Anti-Rat IgG(H+L), Mouse ads-TXRD

Cat. No.:
3050-07
Goat Anti-Rat IgG(H+L)-Texas Red antibody with minimal reactivity to mouse proteins for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.
$182.00
Size Price (USD) Quantity
1.0 mg $182.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-TXRD
Specificity Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Source Pooled antisera from goats hyperimmunized with rat IgG
Cross Adsorption Mouse immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rat IgG covalently linked to agarose
Conjugate TXRD (Texas Red®)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Texas Red® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA 1-4
FLISA
Flow Cytometry 5-10

Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 11-17
Immunohistochemistry-Paraffin Sections 18-20
Imuunocytochemistry 21,22
Western Blot 23-25
Separation 4
RRID Number AB_2795832

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Putterman C, Diamond B. Immunization with a peptide surrogate for double-stranded DNA (dsDNA) induces autoantibody production and renal immunoglobulin deposition. J Exp Med. 1998;188:29-38. (ELISA)
  2. 2. Yu KO, Im JS, Illarianov PA, Ndonye RM, Howell AR, Besra GS, et al. Production and characterization of monoclonal antibodies against complexes of the NKT cell ligand α-galactosylceramide bound to mouse CD1d. J Immunol Methods. 2007;323:11-23. (ELISA)
  3. 3. Swanson CL, Wilson TJ, Strauch P, Colonna M, Pelanda R, Torres RM. Type I IFN enhances follicular B cell contribution to the T cell-independent antibody response. J Exp Med. 2010;207:1485-1500. (ELISA)
  4. 4. Hansen RJ, Brown RM, Lu J, Wroblewski VJ. Qualification of a free ligand assay in the presence of anti-ligand antibody Fab fragments. Mabs. 2013;5:288-96. (ELISA, Sep)
  5. 5. Pittet MJ, Zippelius A, Speiser DE, Assenmacher M, Guillaume P, Valmori D, et al. Ex vivo IFN-γ secretion by circulating CD8 T lymphocytes: implications of a novel approach for T cell monitoring in infectious and malignant diseases. J Immunol. 2001;166:7634-40. (FC)
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  7. 7. Choi K, Kim J, Lee Y, Kim J, Suh B, Kim H, et al. Concurrent delivery of GM-CSF and B7-1 using an oncolytic adenovirus elicits potent antitumor effect. Gene Ther. 2006;13:1010-20. (FC)
  8. 8. Bossen C, Ingold K, Tardivel A, Bodmer J, Gaide O, Hertig S, et al. Interactions of tumor necrosis factor (TNF) and TNF receptor family members in the mouse and human. J Biol Chem. 2006;281:13964-71. (FC)
  9. 9. Ronconi E, Sagrinati C, Angelotti ML, Lazzeri E, Mazzinghi B, Ballerini L, et al. Regeneration of glomerular podocytes by human renal progenitors. J Am Soc Nephrol. 2009;20:322-32. (FC)
  10. 10. Long MA, Rossi FM. Targeted cell fusion facilitates stable heterokaryon generation in vitro and in vivo. PloS One. 2011;6:e26381. (FC)
  11. 11. Csencsits KL, Jutila MA, Pascual DW. Nasal-associated lymphoid tissue: phenotypic and functional evidence for the primary role of peripheral node addressin in naive lymphocyte adhesion to high endothelial venules in a mucosal site. J Immunol. 1999;163:1382-9. (IHC-FS)
  12. 12. Manley HA, Lennon VA. Endoplasmic reticulum membrane-sorting protein of lymphocytes (BAP31) is highly expressed in neurons and discrete endocrine cells. J Histochem Cytochem. 2001;49:1235-43. (IHC-FS)
  13. 13. Yu P, Wang Y, Chin RK, Martinez-Pomares L, Gordon S, Kosco-Vibois MH, et al. B cells control the migration of a subset of dendritic cells into B cell follicles via CXC chemokine ligand 13 in a lymphotoxin-dependent fashion. J Immunol. 2002;168:5117-23. (IHC-FS)
  14. 14. Maehr R, Kraus M, Ploegh HL. Mice deficient in invariant-chain and MHC class II exhibit a normal mature B2 cell compartment. Eur J Immunol. 2004;34:2230-6. (IHC-FS)
  15. 15. He X, Schoeb TR, Panoskaltsis-Mortari A, Zinn KR, Kesterson RA, Zhang J, et al. Deficiency of P-selectin or P-selectin glycoprotein ligand-1 leads to accelerated development of glomerulonephritis and increased expression of CC chemokine ligand 2 in lupus-prone mice. J Immunol. 2006;177:8748-56. (IHC-FS)
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  17. 17. Ubogu EE, Yosef N, Xia RH, Sheikh KA. Behavioral, electrophysiological, and histopathological characterization of a severe murine chronic demyelinating polyneuritis model. J Peripher Nerv Syst. 2012;17:53-61. (IHC-FS)
  18. 18. Cattoretti G, Angelin-Duclos C, Shaknovich R, Zhou H, Wang D, Alobeid B. PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage. J Pathol. 2005;206:76-86. (IHC-PS)
  19. 19. Lunsford KE, Horne PH, Koester MA, Eiring AM, Walker JP, Dziema HL, et al. Activation and maturation of alloreactive CD4-independent, CD8+ cytolytic T cells. Am J Transplant. 2006;6:2268-81. (IHC-PS)
  20. 20. van den Borne P, Rygiel TP, Hoogendoorn A, Westerlaken GH, Boon L, Quax PH, et al. The CD200-CD200 receptor inhibitory axis controls arteriogenesis and local T lymphocyte influx. PLoS One. 2014;9(6):e98820. (IHC-PS)
  21. 21. Elstner E, Linker-Israeli M, Said J, Umiel T, de Vos S, Shintaku IP, et al. 20-epi-vitamin D3 analogues: a novel class of potent inhibitors of proliferation and inducers of differentiation of human breast cancer cell lines. Cancer Res. 1995;55:2822-30. (ICC)
  22. 22. Chung Y, Zhang N, Wooten RM. Borrelia burgdorferi elicited-IL-10 suppresses the production of inflammatory mediators, phagocytosis, and expression of co-stimulatory receptors by murine macrophages and/or dendritic cells. PLoS One. 2013;8(12):e84980. (ICC)
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  24. 24. Ohta K, Mizuno A, Ueda M, Li S, Suzuki Y, Hida Y, et al. Autophagy impairment stimulates PS1 expression and γ-secretase activity. Autophagy. 2010;6:345-52. (WB)
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See All References

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  3. FLISA plate was coated with purified rat IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgM-TXRD (SB Cat. No. 3020-07).
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  4. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  5. FLISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rat IgG(H+L), Mouse ads-TXRD (SB Cat. No. 3052-07).
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