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Goat Anti-Rat IgG(H+L), Mouse ads-BIOT

Goat Anti-Rat IgG(H+L), Mouse ads-BIOT

Name: Goat Anti-Rat IgG(H+L), Mouse ads-BIOT
SKU: 3050-08
Price: 182 USD
Availability: InStock

Cat. No.:

3050-08

Goat Anti-Rat IgG(H+L)-Biotin antibody with minimal reactivity to mouse proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, western blot, and separation assays.

$182.00

Size	Price (USD)	Quantity
1.0 mg	$182.00

Qty

Technical Bulletin Safety Datasheet

Frozen BALB/c mouse intestine tissue was stained with Rat IgG<sub>2a</sub>-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08)

Frozen BALB/c mouse intestine tissue was stained with Rat IgG_2a-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08)

C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

Paraffin embedded mouse adductor muscle day 3 post femoral artery ligation was stained with anti-Mac-3 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-HRP (SB Cat. No. 7100-05), hematoxylin, and AEC.<br/>Image from v

ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

Product Specifications

More Information
Isotype	Goat IgG
Isotype Control	Goat IgG-BIOT
Specificity	Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Source	Pooled antisera from goats hyperimmunized with rat IgG
Cross Adsorption	Mouse immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method	Affinity chromatography on pooled rat IgG covalently linked to agarose
Conjugate	BIOT (Biotin)
Buffer Formulation	Phosphate buffered saline containing < 0.1% sodium azide
Clonality	Polyclonal
Concentration	0.5 mg/mL
Volume	2.0 mL
Recommended Storage	2-8°C
Applications	Quality tested applications for relevant formats include - ELISA ^1-4 FLISA Flow Cytometry ^5-10 Other referenced applications for relevant formats include - Immunohistochemistry-Frozen Sections ^11-17 Immunohistochemistry-Paraffin Sections ^18-20 Imuunocytochemistry ^21,22 Western Blot ^23-25 Separation ⁴
RRID Number	AB_2795833

Documentation

Technical Bulletin Safety Datasheet

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Citations

1. Putterman C, Diamond B. Immunization with a peptide surrogate for double-stranded DNA (dsDNA) induces autoantibody production and renal immunoglobulin deposition. J Exp Med. 1998;188:29-38. (ELISA)
2. Yu KO, Im JS, Illarianov PA, Ndonye RM, Howell AR, Besra GS, et al. Production and characterization of monoclonal antibodies against complexes of the NKT cell ligand α-galactosylceramide bound to mouse CD1d. J Immunol Methods. 2007;323:11-23. (ELISA)
3. Swanson CL, Wilson TJ, Strauch P, Colonna M, Pelanda R, Torres RM. Type I IFN enhances follicular B cell contribution to the T cell-independent antibody response. J Exp Med. 2010;207:1485-1500. (ELISA)
4. Hansen RJ, Brown RM, Lu J, Wroblewski VJ. Qualification of a free ligand assay in the presence of anti-ligand antibody Fab fragments. Mabs. 2013;5:288-96. (ELISA, Sep)
5. Pittet MJ, Zippelius A, Speiser DE, Assenmacher M, Guillaume P, Valmori D, et al. Ex vivo IFN-γ secretion by circulating CD8 T lymphocytes: implications of a novel approach for T cell monitoring in infectious and malignant diseases. J Immunol. 2001;166:7634-40. (FC)
6. Rizzardi GP, Harari A, Capiluppi B, Tambussi G, Ellefsen K, Ciuffreda D, et al. Treatment of primary HIV-1 infection with cyclosporin A coupled with highly active antiretroviral therapy. J Clin Invest. 2002;109:681-8. (FC)
7. Choi K, Kim J, Lee Y, Kim J, Suh B, Kim H, et al. Concurrent delivery of GM-CSF and B7-1 using an oncolytic adenovirus elicits potent antitumor effect. Gene Ther. 2006;13:1010-20. (FC)
8. Bossen C, Ingold K, Tardivel A, Bodmer J, Gaide O, Hertig S, et al. Interactions of tumor necrosis factor (TNF) and TNF receptor family members in the mouse and human. J Biol Chem. 2006;281:13964-71. (FC)
9. Ronconi E, Sagrinati C, Angelotti ML, Lazzeri E, Mazzinghi B, Ballerini L, et al. Regeneration of glomerular podocytes by human renal progenitors. J Am Soc Nephrol. 2009;20:322-32. (FC)
10. Long MA, Rossi FM. Targeted cell fusion facilitates stable heterokaryon generation in vitro and in vivo. PloS One. 2011;6:e26381. (FC)
11. Csencsits KL, Jutila MA, Pascual DW. Nasal-associated lymphoid tissue: phenotypic and functional evidence for the primary role of peripheral node addressin in naive lymphocyte adhesion to high endothelial venules in a mucosal site. J Immunol. 1999;163:1382-9. (IHC-FS)
12. Manley HA, Lennon VA. Endoplasmic reticulum membrane-sorting protein of lymphocytes (BAP31) is highly expressed in neurons and discrete endocrine cells. J Histochem Cytochem. 2001;49:1235-43. (IHC-FS)
13. Yu P, Wang Y, Chin RK, Martinez-Pomares L, Gordon S, Kosco-Vibois MH, et al. B cells control the migration of a subset of dendritic cells into B cell follicles via CXC chemokine ligand 13 in a lymphotoxin-dependent fashion. J Immunol. 2002;168:5117-23. (IHC-FS)
14. Maehr R, Kraus M, Ploegh HL. Mice deficient in invariant-chain and MHC class II exhibit a normal mature B2 cell compartment. Eur J Immunol. 2004;34:2230-6. (IHC-FS)
15. He X, Schoeb TR, Panoskaltsis-Mortari A, Zinn KR, Kesterson RA, Zhang J, et al. Deficiency of P-selectin or P-selectin glycoprotein ligand-1 leads to accelerated development of glomerulonephritis and increased expression of CC chemokine ligand 2 in lupus-prone mice. J Immunol. 2006;177:8748-56. (IHC-FS)
16. El Shikh ME, El Sayed RM, Wu Y, Szakal AK, Tew JG. TLR4 on follicular dendritic cells: an activation pathway that promotes accessory activity. J Immunol. 2007;179:4444-50. (IHC-FS)
17. Ubogu EE, Yosef N, Xia RH, Sheikh KA. Behavioral, electrophysiological, and histopathological characterization of a severe murine chronic demyelinating polyneuritis model. J Peripher Nerv Syst. 2012;17:53-61. (IHC-FS)
18. Cattoretti G, Angelin-Duclos C, Shaknovich R, Zhou H, Wang D, Alobeid B. PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage. J Pathol. 2005;206:76-86. (IHC-PS)
19. Lunsford KE, Horne PH, Koester MA, Eiring AM, Walker JP, Dziema HL, et al. Activation and maturation of alloreactive CD4-independent, CD8⁺ cytolytic T cells. Am J Transplant. 2006;6:2268-81. (IHC-PS)
20. van den Borne P, Rygiel TP, Hoogendoorn A, Westerlaken GH, Boon L, Quax PH, et al. The CD200-CD200 receptor inhibitory axis controls arteriogenesis and local T lymphocyte influx. PLoS One. 2014;9(6):e98820. (IHC-PS)
21. Elstner E, Linker-Israeli M, Said J, Umiel T, de Vos S, Shintaku IP, et al. 20-epi-vitamin D₃ analogues: a novel class of potent inhibitors of proliferation and inducers of differentiation of human breast cancer cell lines. Cancer Res. 1995;55:2822-30. (ICC)
22. Chung Y, Zhang N, Wooten RM. Borrelia burgdorferi elicited-IL-10 suppresses the production of inflammatory mediators, phagocytosis, and expression of co-stimulatory receptors by murine macrophages and/or dendritic cells. PLoS One. 2013;8(12):e84980. (ICC)
23. Tran TM, Satumtira N, Dorris ML, May E, Wang A, Furuta E, et al. HLA-B27 in transgenic rats forms disulfide-linked heavy chain oligomers and multimers that bind to the chaperone BiP. J Immunol. 2004;172:5110-9. (WB)
24. Ohta K, Mizuno A, Ueda M, Li S, Suzuki Y, Hida Y, et al. Autophagy impairment stimulates PS1 expression and γ-secretase activity. Autophagy. 2010;6:345-52. (WB)
25. Rice DS, Hansen GM, Liu F, Crist MJ, Newhouse MM, Potter D, et al. Keratinocyte migration in the developing eyelid requires LIMK2. PLoS One. 2012;7(10):e47168. (WB)