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Goat Anti-Rat IgG-BIOT

Goat Anti-Rat IgG-BIOT

Name: Goat Anti-Rat IgG-BIOT
SKU: 3030-08
Price: 132 USD
Availability: InStock

Cat. No.:

3030-08

Goat Anti-Rat IgG-Biotin antibody for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, and western blot assays.

$132.00

Size	Price (USD)	Quantity
1.0 mg	$132.00

Qty

Technical Bulletin Safety Datasheet

ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

Frozen rat gastrocnemic muscle section was stained with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) followed by a CY3 secondary reagent and DAPI.<br/>Image from Duehrkop C, Banz Y, Spirig R, Miescher S, Nolte MW, Spycher M, et al. C1 esterase inhibitor r

Product Specifications

More Information
Isotype	Goat IgG
Isotype Control	Goat IgG-BIOT
Specificity	Reacts with the heavy chain of rat IgG
Source	Pooled antisera from goats hyperimmunized with rat IgG
Cross Adsorption	Rat IgM; may react with immunoglobulins from other species
Purification Method	Affinity chromatography on pooled rat IgG covalently linked to agarose
Conjugate	BIOT (Biotin)
Buffer Formulation	Phosphate buffered saline containing < 0.1% sodium azide
Clonality	Polyclonal
Concentration	0.5 mg/mL
Volume	2.0 mL
Recommended Storage	2-8°C
Applications	Quality tested applications for relevant formats include - ELISA ^1-5 FLISA Other referenced applications for relevant formats include - Flow Cytometry ^6,7 Immunohistochemistry-Frozen Sections ^8,9 Immunocytochemistry ^10,11 Western Blot ^12-15
RRID Number	AB_2795822

Documentation

Technical Bulletin Safety Datasheet

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Citations

1. Laumonier T, Mohacsi PJ, Matozan KM, Banz Y, Haeberli A, Korchagina EY, et al. Endothelial cell protection by dextran sulfate: a novel strategy to prevent acute vascular rejection in xenotransplantation. Am J Transplant. 2003;4:181-7. (ELISA)
2. Wernhoff P, Olofsson P, Holmdahl R. The genetic control of rheumatoid factor production in a rat model of rheumatoid arthritis. Arthritis Rheum. 2003;48:3584-96. (ELISA)
3. Eto Y, Yoshioka Y, Ishida T, Yao X, Morishige T, Narimatsu S, et al. Optimized PEGylated adenovirus vector reduces the anti-vector humoral immune response against adenovirus and induces a therapeutic effect against metastatic lung cancer. Biol Pharm Bull. 2010;33:1540-4. (ELISA)
4. Cheong C, Matos I, Choi J, Schauer JD, Dandamudi DB, Shrestha E, et al. New monoclonal anti-mouse DC-SIGN antibodies reactive with acetone-fixed cells. J Immunol Methods. 2010;360:66-75. (ELISA)
5. Zuest R, Valdes I, Skibinski D, Lin Y, Toh YX, Chan K, et al. Tetravalent dengue DIIIC protein together with alum and ODN elicits a Th1 response and neutralizing antibodies in mice. Vaccine. 2015;33:1474-82. (ELISA)
6. Achour TN, Sentis S, Teyssier C, Philippat A, Lucas A, Corbo L, et al. Transcriptional repression of estrogen receptor α signaling by SENP2 in breast cancer cells. Mol Endocrinol. 2014;28:183-96. (FC)
7. Scheibe M, Arnoult N, Kappei D, Buchholz F, Decottignies A, Butter F, et al. Quantitative interaction screen of telomeric repeat-containing RNA reveals novel TERRA regulators. Genome Res. 2013;23:2149-57. (FC)
8. Huizinga R, Easton AS, Donachie AM, Guthrie J, van Rijs W, Heikema A, et al. Sialylation of Campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice. PLoS One. 2012;7(3):e34416. (IHC-FS)
9. Duehrkop C, Banz Y, Spirig R, Miescher S, Nolte MW, Spycher M, et al. C1 esterase inhibitor reduces lower extremity ischemia/reperfusion injury and associated lung damage. PLoS One. 2013;8(8):e72059. (IHC-FS)
10. Alliot F, Lecain E, Grima B, Pessac B. Microglial progenitors with a high proliferative potential in the embryonic and adult mouse brain. Proc Natl Acad Sci USA. 1991;88:1541-5. (ICC)
11. Taoudi S, Morrison AM, Inoue H, Gribi R, Ure J, Medvinsky A. Progressive divergence of definitive haematopoietic stem cells from the endothelial compartment does not depend on contact with the foetal liver. Development. 2005;132:4179-91. (ICC)
12. O'Reilly LA, Print C, Hausmann G, Moriishi K, Cory S, Huang DC, et al. Tissue expression and subcellular localization of the pro-survival molecule Bcl-w. Cell Death Differ. 2001;8:486-94. (WB)
13. O'Reilly LA, Ekert P, Harvey N, Marsden V, Cullen L, Vaux DL, et al. Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9. Cell Death Differ. 2002;9:832-41. (WB)
14. Armitage CW, O'Meara CP, Harvie MC, Timms P, Blumberg RS, Beagley KW. Divergent outcomes following transcytosis of IgG targeting intracellular and extracellular chlamydial antigens. Immunol Cell Biol. 2014;92:417-26. (WB)
15. Lou Y, Wu C, Wu M, Xie C, Ren L. The changes of neutrophil gelatinase-associated lipocalin in plasma and its expression in adipose tissue in pregnant women with gestational diabetes. Diabetes Res Clin Pract. 2014;104:136-42. (WB)