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  • Goat Anti-Rat Ig, Mouse ads-BIOT

Goat Anti-Rat Ig, Mouse ads-BIOT

Cat. No.:
3010-08
Goat Anti-Rat Ig-Biotin antibody with minimal reactivity to mouse proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, and western blot assays.
$215.00
Size Price (USD) Quantity
1.0 mg $215.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Specificity Reacts with the heavy and light chains of rat IgG and IgM
Source Pooled antisera from goats hyperimmunized with rat IgG and IgM
Cross Adsorption Mouse immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rat Ig covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1,2
FLISA
Flow Cytometry 3-12

Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 1,13-15
Immunohistochemistry-Paraffin Sections 16,17
Immunocytochemistry 1,4,9
Western Blot 1,2,18-21
Depletion 22
RRID Number AB_2795803

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Yang J, Wang L, Zhang H, Qiu L, Wang H, Song L. C-type lectin in Chlamys farreri (CfLec-1) mediating immune recognition and opsonization. PLoS One. 2011;6(2):e17089. (ELISA, IHC-FS, ICC, WB)
  2. 2. Wang L, Wang L, Zhang H, Zhou Z, Siva VS, Song L. A C1q domain containing protein from scallop Chlamys farreri serving as pattern recognition receptor with heat-aggregated IgG binding activity. PLoS One. 2012;7(8):e43289. (ELISA, WB)
  3. 3. Santos-Argumedo L, Teixeira C, Preece G, Kirkham PA, Parkhouse RM. A B lymphocyte surface molecule mediating activation and protection from apoptosis via calcium channels. J Immunol. 1993;151:3119-30. (FC)
  4. 4. Hess KL, Babcock GF, Askew DS, Cook-Mills JM. A novel flow cytometric method for quantifying phagocytosis of apoptotic cells. Cytometry. 1997;27:145-52. (FC, ICC)
  5. 5. Moritz DR, Rodewald H, Gheyselinck J, Klemenz R. The IL-1 receptor-related T1 antigen is expressed on immature and mature mast cells and on fetal blood mast cell progenitors. J Immunol. 1998;161:4866-74. (FC)
  6. 6. Poltorak A, Ricciardi-Castagnoli P, Citterio S, Beutler B. Physical contact between lipopolysaccharide and toll-like receptor 4 revealed by genetic complementation. Proc Natl Acad Sci USA. 2000;97:2163-7. (FC)
  7. 7. O'Reilly LA, Print C, Hausmann G, Moriishi K, Cory S, Huang DC, et al. Tissue expression and subcellular localization of the pro-survival molecule Bcl-w. Cell Death Differ. 2001;8:486-94. (FC)
  8. 8. Uehara T, Bléry M, Kang D, Chen C, Ho LH, Gartland GL, et al. Inhibition of IgE-mediated mast cell activation by the paired Ig-like receptor PIR-B. J Clin Invest. 2001;108:1041-50. (FC)
  9. 9. O'Reilly LA, Ekert P, Harvey N, Marsden V, Cullen L, Vaux DL, et al. Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9. Cell Death Differ. 2002;9:832-41. (FC, ICC)
  10. 10. Girard R, Pedron T, Uematsu S, Balloy V, Chignard M, Akira S, et al. Lipopolysaccharides from Legionella and Rhizobium stimulate mouse bone marrow granulocytes via Toll-like receptor 2. J Cell Sci. 2003;116:293-302. (FC)
  11. 11. de Haas AH, Boddeke HW, Biber K. Region-specific expression of immunoregulatory proteins on microglia in the healthy CNS. Glia. 2008;56:888-94. (FC)
  12. 12. Bahrami S, Pagh K, Ejegod D, Duch M, Tolstrup M, Pedersen FS. Construction of a gammaretrovirus with a novel tropism and wild-type replication kinetics capable of using human APJ as entry receptor. J Virol. 2012;86:10621-7. (FC)
  13. 13. Zheng B, Han S, Kelsoe G. T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1. J Exp Med. 1996;184:1083-91. (IHC-FS)
  14. 14. Brocker T, Riedinger M, Karjalainen K. Targeted expression of major histocompatibility complex (MHC) class II molecules demonstrates that dendritic cells can induce negative but not positive selection of thymocytes in vivo. J Exp Med. 1997;185:541-50. (IHC-FS)
  15. 15. Perry LL, Feilzer K, Portis JL, Caldwell HD. Distinct homing pathways direct T lymphocytes to the genital and intestinal mucosae in Chlamydia-infected mice. J Immunol. 1998;160:2905-14. (IHC-FS)
  16. 16. Laumonier T, Mohacsi PJ, Matozan KM, Banz Y, Haeberli A, Korchagina EY, et al. Endothelial cell protection by dextran sulfate: a novel strategy to prevent acute vascular rejection in xenotransplantation. Am J Transplant. 2003;4:181-7. (IHC-PS)
  17. 17. Schneider BE, Korbel D, Hagens K, Koch M, Raupach B, Enders J, et al. A role for IL-18 in protective immunity against Mycobacterium tuberculosis. Eur J Immunol. 2010;40:396-405. (IHC-PS)
  18. 18. Apostolaki A, Erpapazoglou Z, Harispe L, Billini M, Kafasla P, Kizis D, et al. AgtA, the dicarboxylic amino acid transporter of Aspergillus nidulans, is concertedly down-regulated by exquisite sensitivity to nitrogen metabolite repression and ammonium-elicited endocytosis. Eukaryot Cell. 2009;8:339-52. (WB)
  19. 19. Chang MY, Smith C, DuHadaway JB, Pyle JR, Boulden J, Soler AP, et al. Cardiac and gastrointestinal liabilities caused by deficiency in the immune modulatory enzyme indoleamine 2,3-dioxygenase. Cancer Biol Ther. 2011;12:1050-8. (WB)
  20. 20. Jiang Q, Zhou Z, Wang L, Wang L, Yue F, Wang J, et al. A scallop nitric oxide synthase (NOS) with structure similar to neuronal NOS and its involvement in the immune defense. PLoS One. 2013;8(7):e69158. (WB)
  21. 21. Chen Y, Jin S, Zhao Q, Zhang Y, Wang C. PtLGBP, a pattern recognition receptor in Portunus trituberculatus involved in the immune response against different challenges. Acta Biol Hung. 2014;65:294-304. (WB)
  22. 22. Landreth KS, Narayanan R, Dorshkind K. Insulin-like growth factor-I regulates pro-B cell differentiation. Blood. 1992;80:1207-12. (Depletion)
See All References

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