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Goat Anti-Rabbit IgM, Multi-Species SP ads-HRP

Cat. No.:
4021-05
Goat Anti-Rabbit IgM-HRP antibody with minimal reactivity to human, mouse, rat, bovine, hamster, goat, sheep, chicken, and guinea pig serum proteins for use in ELISA assays.
$176.00
Size Price (USD) Quantity
1.0 mL $176.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control 0109-05
Specificity Reacts with the heavy chain of rabbit IgM
Source Pooled antisera from goats hyperimmunized with rabbit IgM
Cross Adsorption Rabbit IgG; human, mouse, rat, bovine, hamster, goat, sheep, chicken, and guinea pig serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rabbit IgM covalently linked to agarose
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality Polyclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA

RRID Number AB_2895774

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  2. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05).
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  3. Reduced (Lane 1) and non-reduced rabbit IgG (Lane 2) was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit Light Chain-HRP (SB Cat. No. 4060-05). Light chains were visualized using chemiluminescent detection.
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  4. ELISA plate was coated with serially diluted Rabbit IgM-UNLB (SB Cat. No. 0113-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) and Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  6. Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01). Proteins were visualized using Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 4050-05) secondary antibody and chemiluminescent detection.
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  7. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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